UltraClean DEPC Treated TBS, 1X
Cat No.: DCP-TBSDEPC1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
- World’s cleanest buffer for all cell and molecular biology experiments
Description:
DEPC Treated TBS, 1X (Tris Buffered Saline) is a ready-to-use buffer solution formulated for sensitive molecular biology applications, particularly those involving RNA, where nuclease contamination must be rigorously avoided. This buffer is treated with diethyl pyrocarbonate (DEPC) to inactivate RNases and other nucleases, ensuring the integrity of your RNA samples during experimental workflows.
Applications:
- RNA extraction and purification
- In situ hybridization
- Immunoassays (e.g., western blot, ELISA)
- Any protocol where nuclease-free conditions are critical
Advantages:
- Nuclease-Free: DEPC treatment ensures the buffer is free from RNase, DNase, and other nuclease activity, making it ideal for RNA work and other applications requiring stringent nuclease control
- Sterile and Ready-to-Use: The solution is filtered through a 0.04-micron filter and autoclaved after DEPC treatment to ensure sterility and complete inactivation of residual DEPC, making it immediately suitable for critical experiments
- Physiological Buffer: Maintains a pH of 7.4, mimicking the osmolarity and ion concentrations of the human body, making it compatible with a wide range of cell culture and molecular biology protocols
- Versatile Applicationst: Suitable for use as a wash buffer, storage buffer, sample dilution medium, and for resuspension of cells or nucleic acids in workflows where nuclease contamination must be avoided
Composition:
| Ingredients |
Concentration |
| Tris base |
20 mM |
| Sodium Chloride |
150 mM |
| Water (DEPC-treated, nuclease-free) |
To volume |
Quality Control:
- Appearance: Colorless, clear solution
- pH: 7.4
Storage and Shelf Life:
- Store at 4 °C.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
