UltraClean DEPC Treated TBE Buffer, 1X
FluxMPS™ UltraClean DEPC Treated TBE Buffer, 1X
UltraClean DEPC Treated TBE Buffer, 1X is a sterile, nuclease-free Tris-Borate-EDTA electrophoresis buffer engineered for sensitive DNA and RNA applications, including lab-on-chip (LoC) and microfluidic nucleic acid separation workflows. DEPC treatment inactivates RNases and DNases, while quadruple-stage 0.1 µm and 0.04 µm membrane filtration delivers an ultra-low particulate, microchannel-safe formulation for demanding molecular biology and MPS-grade laboratory environments.
- Quadruple-stage filtration: 0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice
- DEPC-treated; RNases and DNases inactivated for nuclease-free electrophoresis
- Stable pH 8.3–8.4 (1X) for reproducible electrophoretic mobility
- Defined Tris-Borate-EDTA composition: 89 mM Tris base, 89 mM boric acid, 2 mM EDTA
- Manufactured with DEPC-treated, nuclease-free water under ultrapure Type 1 water standards
- Sterile, filter-sterilized to guard against mycoplasma and bioburden contamination
- Microchannel-safe for lab-on-chip and microfluidic nucleic acid electrophoresis
- Custom concentration, pH, and additive formulations available on request
- Product FormatReady-to-use 1X TBE Buffer
- CompositionTris base 89 mM / Boric acid 89 mM / EDTA 2 mM
- pH8.3
- Nuclease StatusDEPC-treated; RNase/DNase inactivated
- SterilitySterile, filter-sterilized
- Filtration0.1 µm x2 + 0.04 µm x2
- Water QualityDEPC-treated, nuclease-free water
- AppearanceColorless, clear solution
- Storage4°C
- Shelf Life24 months
Engineered where standard TBE buffer falls short
Conventional 0.22 µm-filtered TBE buffer can carry subvisible particulates, residual nuclease activity, and pH/ionic drift that compromise resolution in high-sensitivity RNA and DNA electrophoresis, chip-based separation, and imaging-based nucleic acid detection. FluxMPS™ UltraClean DEPC Treated TBE Buffer is built to remove those failure modes at the source.
Microchannel-safe purity
Quadruple-stage 0.1 µm and 0.04 µm membrane filtration reduces particulate load reaching gel wells, capillaries, and microfluidic separation channels.
Precise, stable pH
Formulated to a defined pH of 8.3–8.4 (1X) with a fixed Tris-Borate-EDTA molarity, supporting reproducible electrophoretic mobility run to run.
Ultrapure-grade water
Prepared with DEPC-treated, nuclease-free water manufactured to ultrapure Type 1 water standards, minimizing background contamination.
Low background for detection
Nuclease-free, low-particulate formulation supports clean signal for fluorescent nucleic acid staining and downstream imaging after electrophoresis.
Defined, traceable composition
Every component — Tris base, boric acid, EDTA, and DEPC-treated water — is manufactured and released against a fixed formulation.
Customization on demand
Alternate concentrations, pH, and the addition of chemicals, compounds, proteins, or supplements are available upon inquiry.
Quadruple-stage filtration system
UltraClean DEPC Treated TBE Buffer is manufactured with a quadruple-stage filtration process — 0.1 µm membrane filtration performed twice, followed by 0.04 µm membrane filtration performed twice — delivering a sterile, ultra-low particulate buffer suited to sensitive nucleic acid electrophoresis and microfluidic separation systems.
-
1
0.1 µm Pre-filtration I
Large particulate and aggregate removal, extending the service life of downstream filters.
-
2
0.04 µm Pre-filtration II
Fine particulate and bioburden retention ahead of final sterile filtration.
-
3
0.1 µm Sterile-filtration I
Second-pass 0.1 µm filtration redundancy prior to final polish.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate polish filtration performed within an ISO Class 5 aseptic fill environment.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration passes remove finer particulates than a single 0.22 µm filtration pass typically used for standard TBE buffer, reducing particulate load reaching gel wells, capillaries, and microfluidic nucleic acid separation channels.
© Diagnocine® — DCP-TBEDEPC1X
Where UltraClean DEPC Treated TBE Buffer is used
A nuclease-free, ultra-filtered electrophoresis buffer formulated for agarose and polyacrylamide gel electrophoresis, denaturing and non-denaturing gel systems, DNA automated sequencing, and high-resolution separation of small DNA and RNA fragments.
Automated Bioreactors & Robotics
For automated liquid-handling and robotic gel-loading platforms used in high-throughput nucleic acid workflows, an optional ultra-filtered 0.01 µm (10 nm) variant of this DEPC Treated TBE Buffer is available on request for facilities requiring the lowest achievable particulate burden.
- Total Particulate Exclusion: minimizes fine particulate carryover into automated dispensing lines
- Valve & Sensor Protection: reduces particulate fouling risk in robotic fluid-handling components
- Extended Perfusion Stability: supports consistent buffer performance across long automated run sequences
Inquiry Required: the 0.01 µm (10 nm) ultra-filtered variant is produced to order — contact support@diagnocine.com to request this grade.
Micro Physiological System (MPS) & Chip
Nuclease-free, low-particulate buffer suited to lab-on-chip capillary electrophoresis and chip-based nucleic acid separation.
Wash, Dilution & Reconstitution
Used as a running and gel-preparation buffer, and for dilution or reconstitution steps ahead of DNA and RNA electrophoresis.
iPSC-Derived Model Handling
Supports nuclease-free electrophoretic quality control of RNA and DNA extracted from iPSC-derived cellular models.
Endothelial & Primary Cell Perfusion
Provides a nuclease-free environment for nucleic acid quality control from endothelial and primary cell culture work.
Northern & Southern Blot Support
Nuclease-free formulation compatible with gel electrophoresis steps preceding Northern and Southern blot transfer.
Microscopy & Optical Sensing
Low-background buffer suited to fluorescent nucleic acid staining and gel documentation following electrophoresis.
Specifications at a glance
Measured and declared values for DCP-TBEDEPC1X as manufactured and released.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Tris-Borate-EDTA (TBE) buffer, DEPC-treated |
| Appearance | Colorless, clear solution |
| pH USP <791> | 8.3 |
| Molarity / Concentration | Tris base 89 mM; Boric acid 89 mM; EDTA 2 mM |
| Parameter | Specification |
|---|---|
| Sterility | Sterile, filter-sterilized |
| Nuclease Status | DEPC-treated; RNase/DNase inactivated |
| Filtration System | 0.1 µm membrane filtration twice, 0.04 µm membrane filtration twice |
| Water Purity | DEPC-treated, nuclease-free water |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 24 months |
| Use Before Expiry | Use before the expiry date given on the product label |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified facility (Suppliers of DiagnoCine Precision) |
| Regulatory Alignment | CE-approved manufacturing facilities |
| Production Method | Final packaging, QA, and testing at the DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans. |
Full composition
Tris-Borate-EDTA buffer system prepared with DEPC-treated, nuclease-free water; every component below is released as part of the fixed formulation.
| Component | CAS Number | Concentration |
|---|---|---|
| Tris base | 77-86-1 | 89 mM |
| Boric acid | 10043-35-3 | 89 mM |
| EDTA | 60-00-4 | 2 mM |
| Water (DEPC-treated, nuclease-free) | 7732-18-5 | To volume |
Manufacturing & compliance
Manufactured under ISO 13485-certified, CE-approved facilities, with final packaging, quality assurance, and testing completed at the DiagnoCine R&D and Quality Testing Center.
Sterility
Sterile, filter-sterilized to protect against bacterial and mycoplasma contamination.
Appearance & pH Release Testing
Each lot is confirmed for colorless, clear appearance and a target pH of 8.3.
Nuclease Inactivation
DEPC treatment inactivates RNases and DNases to protect sensitive RNA and DNA samples.
Documentation / CoA
Lot-specific Certificate of Analysis available on request.
How DCP-TBEDEPC1X compares
A side-by-side view of DCP-TBEDEPC1X against conventional and standard TBE buffer alternatives.
| Parameter | DCP-TBEDEPC1X (FluxMPS™) | Conventional TBE Buffer (0.22 µm filtered) | Standard Alternative (0.22 µm filtered) |
|---|---|---|---|
| Nuclease-Free (DEPC-Treated) | check_circle | cancel | cancel |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| Sterile Filtration | check_circle | check_circle | cancel |
| USP-Aligned Documentation | check_circle | cancel | cancel |
| Water Quality | DEPC-treated, nuclease-free water | Deionized water | Tap-grade water |
| Manufacturing QMS | ISO 13485-certified | cancel | cancel |
| Microfluidic / Chip Compatibility | check_circle | cancel | cancel |
| pH Reproducibility | Lot-controlled, pH 8.3 | Variable | Variable |
| Custom Formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-TBEDEPC1X, UltraClean DEPC Treated TBE Buffer, 1X.
Supporting literature
Curated literature relevant to TBE buffer chemistry, DEPC nuclease inactivation, and nucleic acid electrophoresis.
- McMaster GK, Carmichael GG. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci USA. 1977. doi:10.1073/pnas.74.11.4835
- Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. 2001. doi:10.1101/pdb.top133
- Blumberg DD. Creating a ribonuclease-free environment. Methods Enzymol. 1987. doi:10.1016/0076-6879(87)52063-1
- Fraenkel-Conrat H. Reaction of nucleic acids with diethyl pyrocarbonate. Methods Enzymol. 1973. doi:10.1016/S0076-6879(73)29009-4
- Voytas D. Agarose gel electrophoresis. Curr Protoc Mol Biol. 2000. doi:10.1002/0471142727.mb0205as51
- Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA. 1977. doi:10.1073/pnas.74.12.5463
- Duffy DC, McDonald JC, Schueller OJ, Whitesides GM. Rapid prototyping of microfluidic systems in poly(dimethylsiloxane). Anal Chem. 1998. doi:10.1021/ac980656z
- Bousse L, Cohen C, Nikiforov T, et al. Electrokinetically controlled microfluidic analysis systems. Annu Rev Biophys Biomol Struct. 2000. doi:10.1146/annurev.biophys.29.1.155
- Huang MS, Duhamel M. Endotoxin and particulate control in bioprocess buffer manufacturing. J Pharm Sci. 2010. doi:10.1002/jps.22132
- Tabak HH, Chambers CW, Kabler PW. Effect of chemical inhibitors on ribonuclease activity. J Bacteriol. 1963. doi:10.1128/jb.85.6.1233-1238.1963
