What makes the filtration superior to standard 0.22 micron-filtered buffer?

DCP-PBSDEPC1X is filtered through a 0.1 micron membrane twice and a 0.04 micron membrane twice, which removes finer particulates than a single 0.22 micron pass used in conventional buffers.

What pH, molarity, and ionic strength should I expect, and can these be adjusted for my protocol?

The buffer is formulated to a physiological pH of 7.0-7.4 (measured at 7.4) with 137 mM NaCl, 2.7 mM KCl, 10.1 mM Na2HPO4, and 1.76 mM KH2PO4. Alternate concentrations and pH are available on request.

At what temperature is the pH specified, and how stable is it during storage and use?

A specific pH testing temperature is not published for this product. The buffer is stored at 4 degrees C and carries a 24-month shelf life when used before the expiry date on the product label.

What endotoxin level should I expect, and how is it verified?

Endotoxin is assessed as part of routine quality control per the USP less than 85 greater than Bacterial Endotoxins Test protocol. Lot-specific values are available on the Certificate of Analysis.

UltraClean DEPC Treated PBS, 1X (Phosphate Buffered Saline)

Product Overview

ISO 13485 Certified Manufacturing

FluxMPS™ UltraClean DEPC Treated PBS, 1X (Phosphate Buffered Saline)

DCP-PBSDEPC1X is an MPS-grade, DEPC-treated 1X phosphate buffered saline engineered for RNase- and DNase-free molecular biology workflows.^[1] Formulated to a physiological pH and purified through a quadruple-stage 0.1 µm and 0.04 µm filtration process, it delivers ultra-clean, nuclease-free performance for microfluidic, organ-on-a-chip, and nucleic acid research applications.

Quadruple-stage filtration: 0.1 µm membrane filtered twice and 0.04 µm membrane filtered twice for ultra-low particulate purity
DEPC-treated to inactivate RNase and DNase, then autoclaved to ensure sterility and complete removal of residual DEPC
Physiological pH of 7.0–7.4, formulated with NaCl, KCl, Na₂HPO₄, and KH₂PO₄
Prepared to volume with DEPC-treated, nuclease-free, Ultrapure Type 1 water (18.2 MΩ·cm)
Sterile, colorless, clear solution ready for use in sensitive molecular biology applications
Compatible with RNA/DNA isolation, RT-PCR, qPCR, and in vitro transcription workflows
Manufactured under ISO 13485-certified, CE-approved facilities
pH, concentration, additives, and formulation customization available on request

SKU: DCP-PBSDEPC1X | UNSPSC 12352204 - Nucleic Acids RNA Works Buffers

UltraClean DEPC Treated PBS, 1X — Sterile, RNase/DNase-Free Buffer

pH
7.4
Formulation
1X PBS (NaCl, KCl, Na₂HPO₄, KH₂PO₄)
Nuclease Status
RNase- and DNase-free (DEPC-treated)
Water Quality
Ultrapure Type 1 (18.2 MΩ·cm), DEPC-treated
Sterility
Sterile
Filtration
0.1 µm x2 + 0.04 µm x2 (Quadruple-stage)
Appearance
Colorless, clear solution
Storage
4°C
Shelf Life
24 months
Manufacturing
ISO 13485-certified, CE-approved

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard buffers fail

Conventional 0.22 µm-filtered buffers can carry subvisible particulates, residual nucleases, and pH or ionic drift that compromise sensitive nucleic acid workflows. DCP-PBSDEPC1X is built to remove those failure points at the source.

filter_alt

Microchannel-Safe, Nuclease-Free Purity

Quadruple-stage 0.1 µm and 0.04 µm filtration combined with DEPC treatment removes fine particulates and inactivates RNase/DNase for microfluidic-safe use.

target

Precise, Physiological pH

Formulated to pH 7.0–7.4 with defined NaCl, KCl, Na₂HPO₄, and KH₂PO₄ concentrations mimicking physiological ion balance.

water_drop

Ultrapure DEPC-Treated Water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) treated with diethyl pyrocarbonate to inactivate residual nucleases.

visibility

Low-Background for Sensitive Assays

Nuclease-free, low-particulate formulation supports clean signal in RT-PCR, qPCR, and in vitro transcription reactions.

science

Defined, Traceable Composition

Lot-released NaCl, KCl, Na₂HPO₄, and KH₂PO₄ in DEPC-treated, nuclease-free water.

tune

Customization on Demand

Alternate concentrations, additives, pH, and modifications available — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

DCP-PBSDEPC1X is sterile-filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice, then DEPC-treated and autoclaved — supporting the ultra-low particulate, nuclease-free background required for RNA and DNA workflows.

1

0.1 µm Pre-filtration I

Removes large particulates and aggregates, extending downstream filter life.
2

0.04 µm Pre-filtration II

Retains fine particulates and bioburden, including organisms as small as mycoplasma, which can measure approximately 0.2 micron.
3

0.1 µm Sterile-filtration I

Second-pass redundancy through a 0.1 µm membrane ahead of final polishing.
4

0.04 µm Sterile-filtration II — Final Polish

Final 0.04 µm polish performed under ISO Class 5 (Class 100) aseptic fill conditions.

Performance vs. conventional buffer

Sequential 0.1 µm and 0.04 µm filtration, each applied twice, removes finer particulates than a single 0.22 µm pass, supporting the low-particulate, nuclease-free background this buffer is designed to deliver for nucleic acid workflows.

0.04 µm

Final filtration stage

4

Total filtration stages

After DEPC treatment, the buffer is filtered through a 0.04 µm filter and autoclaved to ensure sterility (USP <71>) and complete inactivation of residual DEPC, preventing mycoplasma contamination in the finished solution.

DCP-PBSDEPC1X FluxMPS quadruple-stage 0.1 micron and 0.04 micron filtration diagram for DEPC treated PBS used in organ-on-a-chip and microfluidic RNA and DNA molecular biology applications — **Figure 1.** Quadruple-stage filtration architecture (0.1 µm membrane twice, 0.04 µm membrane twice) used to produce DCP-PBSDEPC1X.
© Diagnocine® — DCP-PBSDEPC1X

Applications

Built for nuclease-sensitive research workflows

DCP-PBSDEPC1X supports RNA and DNA isolation and purification, sample preparation for RT-PCR, qPCR, and in vitro transcription, and protein research and immunoassays where nuclease-free conditions are required.^[2]

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated perfusion and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available to further protect valves, sensors, and microchannels from particulate accumulation.

Total Particulate Exclusion: 0.01 µm ultra-filtration for the finest downstream particulate protection
Valve & Sensor Protection: minimizes microparticulate load on automated flow components
Extended Perfusion Stability: supports longer unattended run times in closed perfusion loops

Inquiry Required: the 0.01 µm ultra-filtered grade is available upon request — contact support@diagnocine.com.

Microfluidics

Micro Physiological System (MPS) & Chip

Nuclease-free, ultra-filtered buffer for perfusion and reagent handling in microfluidic chip formats.

OoCToCBoCLoCMPS

Sample Preparation

RNA & DNA Isolation and Purification

Wash and dilution buffer for nucleic acid isolation workflows requiring RNase- and DNase-free conditions.

LysisDilutionReconstitutionRinse

Stem Cell Biology

iPSC-Derived Model Handling

Nuclease-free wash and resuspension buffer for handling iPSC-derived cell models.

iPSC-NeuronsiPSC-CMiPSC-Hep

Vascular Biology

Endothelial & Primary Cell Perfusion

Physiological pH and ionic composition suitable for perfusion of primary and endothelial cell models.

HUVECsHAECsPrimary hepatocytes

Immunoassays

Protein Research & Nuclease-Free Immunoassays

Sterile, nuclease-free buffer for protein research and immunoassays where nuclease contamination must be avoided.

ELISAWestern blotIHCIF

Molecular Biology

RT-PCR, qPCR & In Vitro Transcription

Nuclease-free sample preparation buffer supporting enzymatic reactions sensitive to RNase/DNase activity.

RT-PCRqPCRIVT

Technical Specifications

Full specification sheet

Measured and declared parameters for DCP-PBSDEPC1X.

Physical & Chemical Parameters

Parameter	Specification
Formulation / Composition	1X PBS (NaCl, KCl, Na₂HPO₄, KH₂PO₄) in DEPC-treated, nuclease-free water
Appearance	Colorless, clear solution
pH (USP <791>)	7.4

Sterility, Purity & Safety Parameters

Parameter	Specification
Sterility (USP <71>) USP	Sterile
Water Quality	Ultrapure Type 1 water (18.2 MΩ·cm), DEPC-treated
Manufacturing Standard ISO	ISO 13485-certified, CE-approved facilities
Fill Environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage Temperature	4°C
Shelf Life	24 months

Raw Materials & Regulatory Traceability

Parameter	Specification
Raw Material Grade	Ultrapure, DEPC-treated
Traceability	Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center; customization and assembly performed at DiagnoCine Precision, Totowa, New Jersey, USA
Manufacturing QMS ISO	ISO 13485-certified, CE-approved facilities (Suppliers of DiagnoCine Precision)
Regulatory Alignment	For Research Use Only (RUO)
Production Method	DEPC treatment to inactivate RNase and DNase, followed by 0.04 µm filtration and autoclaving
Intended Use	RNA and DNA isolation, RT-PCR, qPCR, in vitro transcription, and nuclease-free molecular biology applications

Formulation

Full composition

Component concentrations as formulated in DCP-PBSDEPC1X, prepared to volume in DEPC-treated, nuclease-free water.

Component	CAS Number	Concentration
Sodium Chloride (NaCl)	7647-14-5	137 mM
Potassium Chloride (KCl)	7447-40-7	2.7 mM
Sodium Phosphate Dibasic (Na₂HPO₄)	7558-79-4	10.1 mM
Potassium Phosphate Monobasic (KH₂PO₄)	7778-77-0	1.76 mM
Water (DEPC-treated, nuclease-free)	7732-18-5	To volume

Alternate concentrations, additions of chemicals, compounds, proteins, or supplements, different pH, and other modifications are available on request — contact support@diagnocine.com.

Quality Assurance

Manufactured under certified quality systems

DCP-PBSDEPC1X is manufactured under ISO 13485-certified and CE-approved facilities, with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.

verified

ISO 13485:2016 QMS

Manufactured under an ISO 13485-certified, CE-approved quality management system.

water_drop

Ultrapure Type 1 Water

Prepared with DEPC-treated, nuclease-free Ultrapure Type 1 water (18.2 MΩ·cm).

biotech

ISO Class 5 Fill & Finish

Final 0.04 µm filtration polish performed under ISO Class 5 (Class 100) aseptic conditions.

assignment

Micro-Batch Precision

Customization requests and assembly performed at DiagnoCine Precision in Totowa, New Jersey, USA.

Endotoxin (USP <85> BET)

Tested per the USP <85> Bacterial Endotoxins Test protocol as part of routine quality control.

Particulate (USP <788> Method 2)

Quadruple-stage 0.1 µm and 0.04 µm filtration supports low-particulate quality consistent with USP <788> expectations.

Osmolality (USP <785>)

Formulated to a physiological ion balance in accordance with USP <785> testing methodology.

Documentation / CoA

Certificate of Analysis available upon request for lot-specific data.

Request a Certificate of Analysis for this lot at support@diagnocine.com.

Product Comparison

How DCP-PBSDEPC1X compares

A side-by-side look at DCP-PBSDEPC1X against conventional 0.22 µm-filtered buffers.

Parameter	DCP-PBSDEPC1X (FluxMPS™)	Conventional Buffer	Standard Alternative
RNase/DNase-Free (DEPC-Treated)	check_circle	cancel	cancel
Final Filtration Pore Size	0.04 µm	0.22 µm	0.22 µm
Number of Filtration Stages	4	1	1
Sterile	check_circle	check_circle	check_circle
Physiological pH 7.0–7.4	check_circle	cancel	cancel
Water Quality	Ultrapure Type 1, DEPC-treated	Standard purified	Standard purified
Manufacturing QMS	ISO 13485 / CE	Unspecified	Unspecified
Microfluidic Channel Compatibility	check_circle	cancel	cancel
Custom Formulation	check_circle	cancel	cancel

FAQ

Frequently asked questions

Common questions about DCP-PBSDEPC1X.

Yes. Its quadruple-stage filtration and nuclease-free formulation make it suitable for use in microfluidic, organ-on-a-chip (OoC), and other MPS-based platforms handling nucleic acids.

DCP-PBSDEPC1X is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice, which removes finer particulates than a single 0.22 µm pass used in conventional buffers.

The buffer is formulated to a physiological pH of 7.0–7.4 (measured at 7.4) with 137 mM NaCl, 2.7 mM KCl, 10.1 mM Na₂HPO₄, and 1.76 mM KH₂PO₄. Alternate concentrations and pH are available on request.

A specific pH testing temperature is not published for this product. The buffer is stored at 4°C and carries a 24-month shelf life when used before the expiry date on the product label.

Yes. Additions of chemicals, compounds, proteins, or supplements, along with different pH or concentration, can be arranged — contact support@diagnocine.com to inquire.

Endotoxin is assessed as part of routine quality control per the USP <85> Bacterial Endotoxins Test protocol. Lot-specific values are available on the Certificate of Analysis.

Yes. A CoA is available upon request, reflecting lot-specific quality control testing performed at the DiagnoCine R&D and Quality Testing Center. Contact support@diagnocine.com.

Scientific References

Supporting literature

Curated literature relevant to DEPC-treated buffers, PBS chemistry, and organ-on-a-chip / microfluidic applications.

Ingber DE. Human organs-on-chips for disease modeling, drug discovery and personalized medicine. Nat Rev Genet. 2022;23:467-491. doi:10.1038/s41576-022-00466-9
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
Kim HJ, Huh D, Hamilton G, Ingber DE. Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow. Lab Chip. 2012;12:2165-2174. doi:10.1039/c2lc40074j
Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20:345-361. doi:10.1038/s41573-020-0079-3
Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987;162:156-159. doi:10.1016/0003-2697(87)90021-2
Blumberg DD. Creating a ribonuclease-free environment. Methods Enzymol. 1987;152:20-24. doi:10.1016/0076-6879(87)52005-8
Green MR, Sambrook J. Diethyl pyrocarbonate (DEPC) treatment to inactivate RNases. Cold Spring Harb Protoc. 2019;2019. doi:10.1101/pdb.prot101659
Dulbecco R, Vogt M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954;99:167-182. doi:10.1084/jem.99.2.167
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55-63. doi:10.1016/0022-1759(83)90303-4