UltraClean DEPC Treated MOPS Buffer, 1X
Cat No.: DCP-MOPSDEPC1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest buffer for all cell and molecular biology experiments
Description:
DEPC Treated MOPS Buffer, 1X is a high-purity, nuclease-free buffer solution designed for sensitive molecular biology applications, especially those involving RNA. Treated with diethyl pyrocarbonate (DEPC) and sterile-filtered, this buffer ensures the inactivation of RNases and DNases, providing maximum protection for RNA and DNA samples during electrophoresis and other workflows.
Applications:
- RNA Electrophoresis: Ideal for agarose gel electrophoresis of RNA, such as in Northern blot analysis, due to its ability to maintain RNA integrity in a nuclease-free environment
- Sample Preparation: Used in the preparation and handling of RNA and DNA samples to prevent nuclease-mediated degradation
- Protein Purification: Suitable for use as a buffer in protein purification protocols and chromatography where nuclease-free conditions are required
- Cell Culture Media: Can be used as a buffering agent in cell culture media for bacteria, yeast, and mammalian cells
Advantages:
- Nuclease-Free: DEPC treatment inactivates RNases and DNases, ensuring the buffer is suitable for RNA work and other nuclease-sensitive applications
- High Purity: Manufactured using molecular biology-grade reagents and sterile filtration to guarantee the absence of contaminants
Composition:
| Ingredients |
Concentration |
| MOPS |
20 mM |
| Sodium Acetate |
5 mM |
| EDTA |
1 mM |
| Water (DEPC-treated, nuclease-free) |
To volume |
Quality Control:
- Appearance: Colorless, clear solution
- pH: 7.0
Storage and Shelf Life:
- Store at 4 °C.
- Shelf life is 12 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
