Cytoskeleton Extraction Buffer [1X]
FluxMPS™ Cytoskeleton Extraction Buffer [1X]
An MPS-grade, Tris-HCl buffered (pH 7.4) detergent-based extraction reagent engineered to selectively solubilize and enrich cytoskeletal and focal-adhesion-associated proteins from cultured cells. Quadruple-stage filtered (0.1 µm membrane twice, 0.04 µm membrane twice) with Ultrapure Type 1 water for microchannel-safe, low-background proteomic workflows.
- Quadruple-stage filtration: 0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice for ultra-low particulate quality
- Precise pH 7.4 Tris-HCl (10 mM) buffering system with 100 mM NaCl ionic strength control
- Detergent-based extraction chemistry (1% Triton X-100, 0.1% SDS, 0.5% deoxycholate) for selective cytoskeletal protein enrichment
- Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm) under ISO Class 5 (Class 100) aseptic fill
- No detectable DNase or RNase activity after 18-hour incubation at room temperature
- Custom pH, molarity, and additive formulations available on request
- pH7.4
- Buffer SystemTris-HCl, 10 mM
- Ionic StrengthNaCl, 100 mM
- Chelating AgentsEDTA 1 mM, EGTA 1 mM
- Detergent ContentTriton X-100 1%, SDS 0.1%, Deoxycholate 0.5%
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- SterilityFiltered in a sterile environment
- Storage4°C
- Shelf Life12 months
- AppearanceClear, colorless liquid
Engineered where standard extraction buffers fail
Conventional cell lysis buffers such as RIPA are formulated for nuclear, cytoplasmic, and mitochondrial protein extraction, leaving cytoskeleton-bound proteins insoluble and underrepresented in downstream analysis. This buffer is purpose-built with a detergent system that solubilizes cytoskeletal and focal-adhesion-associated proteins while removing soluble cytoplasmic and nuclear background, all within an ultra-filtered, microchannel-safe formulation.
Microchannel-safe purity
Quadruple-stage filtration (0.1 µm membrane twice, 0.04 µm membrane twice) delivers ultra-low particulate buffer suitable for downstream mass spectrometry and microfluidic sample handling.
Precise, stable pH
Tris-HCl (10 mM) buffering holds pH at 7.4, preserving cytoskeletal protein conformation and post-translational modifications during extraction.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), aligned with USP <85> expectations for reagent purity.
Low background for imaging & assays
Verified free of detectable DNase and RNase activity, minimizing background interference in downstream western blot and mass spectrometry proteomic workflows.
Defined, traceable composition
An 11-component formulation of buffer salts, chelators, phosphatase inhibitors, and detergents, each concentration lot-controlled for reproducible cytoskeletal protein enrichment.
Customization on demand
Alternate concentrations, pH, and additional chemicals, compounds, proteins, or supplements can be formulated on request.
Quadruple-stage filtration system
Every lot of Cytoskeleton Extraction Buffer [1X] passes through four sequential filtration stages — 0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice — performed in a sterile environment to deliver the world's cleanest buffer available for cell and molecular biology experiments.
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1
0.1 µm Pre-filtration I
Removes large particulates and aggregates from the buffer, extending the working life of downstream filtration membranes.
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2
0.04 µm Pre-filtration II
Retains fine particulates and bioburden. The smallest mycoplasma type is approximately 0.2 microns, so filtration at this pore size helps prevent mycoplasma contamination in the finished buffer.
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3
0.1 µm Sterile-filtration I
A second 0.1 µm pass provides redundant particulate control ahead of final sterile filtration.
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4
0.04 µm Sterile-filtration II — Final Polish
The final 0.04 µm pass is performed under ISO Class 5 (Class 100) aseptic fill conditions, completing the quadruple-stage sterile filtration process.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, repeated twice each, removes finer particulates than a single-pass 0.22 µm filtered buffer, supporting cleaner downstream mass spectrometry and microfluidic sample handling.
© Diagnocine® — DCP-CEB1X
Where cytoskeletal protein extraction matters
Designed for selective isolation and enrichment of cytoskeleton-bound proteins for downstream molecular and cell biology analysis, this buffer supports workflows ranging from bench-top western blotting to microfluidic and organ-on-a-chip (OoC) sample preparation.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant is available for automated bioreactor and robotic liquid-handling systems where sub-0.04 µm particulate control is required to protect fine-bore valves and sensors.
- Total Particulate Exclusion for automated fluid-handling pathways
- Valve & Sensor Protection in robotic liquid-handling platforms
- Extended Perfusion Stability for long-run automated protocols
Inquiry Required: the 0.01 µm (10 nm) ultra-filtered grade is available on request — contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Ultra-filtered formulation supports cytoskeletal protein extraction within organ-on-a-chip and tissue-on-a-chip sample preparation workflows.
Wash, Dilution & Reconstitution
Used to lyse cells grown in culture dishes after a PBS wash, retaining insoluble cytoskeletal proteins on the dish while soluble proteins are collected in the lysate.
iPSC-Derived Model Handling
Applicable to cytoskeletal protein profiling in iPSC-derived cell models where preservation of focal adhesion and actin-associated proteins is required.
Endothelial & Primary Cell Perfusion
Supports cytoskeletal and focal-adhesion protein extraction from endothelial and primary cell monolayers used in perfusion-based vascular models.
ELISA, Blotting & Blocking
Cytoskeletal protein extracts prepared with this buffer are compatible with downstream western blotting and immunodetection workflows.
Microscopy & Optical Sensing
Low-background composition supports subsequent immunofluorescence and confocal imaging of cytoskeletal architecture following protein extraction.
Physical, chemical & quality parameters
Specifications for DCP-CEB1X as manufactured and quality-tested by Diagnocine.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Detergent-based Tris-HCl cytoskeleton extraction buffer |
| Appearance | Clear, colorless liquid |
| pH USP <791> | 7.4 |
| Tris-HCl concentration | 10 mM |
| NaCl concentration (ionic strength) | 100 mM |
| Chelator concentration | EDTA 1 mM; EGTA 1 mM |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a sterile environment |
| DNase Activity | None detected after 18 hr incubation with plasmid DNA at room temperature |
| RNase Activity | None detected after 18 hr incubation with ribosomal RNA at room temperature |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved supplier facilities |
| Fill Environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage Temperature | 4°C |
| Shelf Life | 12 months |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified |
| Regulatory Alignment | CE-approved facilities |
| Production Method | Final packaging, quality assurance, and testing performed at the Diagnocine R&D and Quality Testing Center |
| Traceability | Customization and assembly completed at Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Detergent-based Tris-HCl extraction buffer formulated to retain focal adhesion and actin-associated proteins while removing soluble cytoplasmic and nuclear proteins, with protease and phosphatase inhibitors to preserve post-translational modifications.
| Component | CAS Number | Concentration |
|---|---|---|
| Tris-HCl | 1185-53-1 | 10 mM |
| NaCl | 7647-14-5 | 100 mM |
| EDTA | 60-00-4 | 1 mM |
| EGTA | 67-42-5 | 1 mM |
| NaF | 7681-49-4 | 1 mM |
| Na4P2O7 | 7722-88-5 | 20 mM |
| Na3VO4 | 13721-39-6 | 2 mM |
| Triton X-100 | 9036-19-5 | 1% |
| Glycerol | 56-81-5 | 10% |
| SDS | 151-21-3 | 0.1% |
| Deoxycholate | 302-95-4 | 0.5% |
Manufacturing & compliance
Manufactured under ISO 13485-certified and CE-approved supplier facilities, with final packaging, quality assurance, and testing performed at the Diagnocine R&D and Quality Testing Center.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved supplier facilities (Suppliers of Diagnocine Precision).
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) supporting USP <85> reagent purity standards.
ISO Class 5 Fill & Finish
Aseptically filtered and filled in an ISO Class 5 (Class 100) environment to help prevent mycoplasma contamination.
Micro-Batch Precision
All specific customization requests and assembly are completed at Diagnocine Precision in Totowa, New Jersey, USA.
DNase Activity
None detected after 18-hour incubation of plasmid DNA with this product at room temperature.
RNase Activity
No RNase activity detected after 18-hour incubation of ribosomal RNA with this product at room temperature.
Sterility USP <71>
Filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice in a sterile environment.
Documentation / CoA
Certificate of Analysis available upon request.
How DCP-CEB1X compares
DCP-CEB1X against conventional single-stage filtered extraction buffers.
| Parameter | DCP-CEB1X (FluxMPS™) | Conventional (0.22 µm filtered) | Standard alternative (0.22 µm filtered) |
|---|---|---|---|
| Extraction chemistry | Detergent-based Tris-HCl (1% Triton X-100, 0.1% SDS, 0.5% deoxycholate) | Generic lysis buffer | Generic lysis buffer |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| DNase/RNase activity verification | check_circle None detected | cancel | cancel |
| USP particulate compliance USP <788> | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1 water (18.2 MΩ·cm) | Standard purified water | Standard purified water |
| Manufacturing QMS | ISO 13485-certified, CE-approved | Unspecified | Unspecified |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-CEB1X Cytoskeleton Extraction Buffer [1X].
Supporting literature
Curated literature relevant to cytoskeletal protein extraction, detergent-based lysis, and buffer chemistry.
- Fey EG, et al. "The nonchromatin substructures of the nucleus: the ribonucleoprotein (RNP)-containing and RNP-depleted matrices analyzed by sequential fractionation." J Cell Biol. doi:10.1083/jcb.102.5.1654
- Small JV, et al. "Assembling an actin cytoskeleton for cell attachment and movement." Biochim Biophys Acta. doi:10.1016/S0167-4889(99)00105-0
- Kuo JC, et al. "Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for beta-Pix in negative regulation of focal adhesion maturation." Nat Cell Biol. doi:10.1038/ncb2055
- Ramsby ML, Makowski GS. "Differential detergent fractionation of eukaryotic cells: analysis by two-dimensional gel electrophoresis." Methods Mol Biol. doi:10.1385/1-59259-584-7:53
- Huttlin EL, et al. "Architecture of the human interactome defines protein communities and disease networks." Nature. doi:10.1038/nature22366
- Good MC, et al. "Scaffold proteins: hubs for controlling the flow of cellular information." Science. doi:10.1126/science.1191184
- Bhattacharya D, et al. "A high-throughput assay for detecting sterility and endotoxin control in filtered laboratory reagents." J Pharm Sci. doi:10.1002/jps.23917
- Bhatia SN, Ingber DE. "Microfluidic organs-on-chips." Nat Biotechnol. doi:10.1038/nbt.2989
- Wisniewski JR, et al. "Universal sample preparation method for proteome analysis." Nat Methods. doi:10.1038/nmeth.1322
- Good NE, et al. "Hydrogen ion buffers for biological research." Biochemistry. doi:10.1021/bi00866a011
