Coomassie Blue Staining Solution
Cat No.: DCP-CS1X
Size: 2 x 500mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
A high-sensitivity, colloidal protein stain optimized for rapid visualization of proteins in SDS-PAGE gels. Designed for researchers prioritizing speed, environmental safety, and downstream compatibility.
Applications:
- High-resolution protein detection in 1D/2D gels
- Pre-mass spectrometry quality control
- Educational labs (simplified safety protocols)
- Green chemistry workflows (no regulated waste)
Advantages:
- Simplicity: Easy-to-use, single-reagent staining protocol
- Mass spectrometry compatible: Ideal for proteomics research
- High reproducibility: Endpoint staining method ensures consistent results
- Wide linear range: Suitable for both qualitative and quantitative protein analysis
- Non-covalent binding: Allows for protein recovery after staining
- Environmentally friendly: Reduced hazardous waste compared to traditional formulations
Composition:
| Ingredients |
Concentration |
| Coomassie Blue (G-250) |
0.1% |
Comparison with Coomassie Blue (R-250):
| Feature |
Coomassie Blue (G-250) |
Coomassie Blue (R-250) |
| Sensitivity |
8–10 ng/band |
50–100 ng/band |
| Toxicity |
Non-hazardous |
Hazardous (MeOH/HOAc) |
| Processing Time |
1.5–2.5 hrs |
4–6 hrs |
Protocol
| Action |
Time |
Key Notes |
Post-electrophoresis rinse:
Wash gel 3× with ultrapure water (100 mL per wash) |
5 min per wash |
Removes residual SDS and buffers |
Microwave pre-treatment:
Submerge gel in 100 mL water. Microwave at 800W for 1 min/gel |
1–2 min |
Ensures even heating; avoid boiling |
Staining:
Replace water with 50–100 mL staining solution. Microwave at 800W for 30 sec/gel |
30 sec |
Solution turns dark blue; proteins bind dye rapidly |
Agitation:
Shake gently on orbital shaker |
15–30 min |
Bands should be visible within 5-10 min |
Destaining:
Replace stain with water. Microwave at 800W for 1 min/gel, then shake for 1-2 hours |
1 min + 1-2 hours |
Add Kimwipes® to absorb excess dye |
Quality Control:
-
Appearance: Blue Liquid (amber bottle).
-
pH: 2.0
-
Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
-
DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
-
RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
Safety and Handling:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
