Columbia Agar Base Plates/W Tetracycline
Catalog No: DCP-CABPT
pH: 7.3 ± 0.2
Uses: Standard method base for enumeration and detection of bacteria in food and environmental samples, and clinical speciments
Size: 100mm
Concentration: 20 µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: light amber, clear to slightly opalescent firm gel. Adding 5% sheep (or horse/rabbit) blood, the medium becomes opaque red
Sterile
Shelf life: 4 Weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish
Description:
Columbia Agar Base with tetracycline is a nutrient-rich, non-blood agar medium in which standard Columbia agar base is supplemented with the broad-spectrum antibiotic tetracycline to make the medium selective by inhibiting tetracycline-susceptible bacteria while allowing tetracycline-resistant organisms to grow. Columbia agar base is a complex peptone medium originally devised as a blood-agar base for the cultivation of fastidious organisms (such as streptococci, staphylococci, Neisseria, and Haemophilus) as well as many non-fastidious bacteria. Peptones and yeast extract provide amino acids, nitrogen, peptides, and B-complex vitamins support rapid bacterial growth and well-defined colony morphology. Starch works as a carbon and energy source and is able to bind or neutralize toxic metabolites. NaCl maintians osmotic balance.
Tetracycline is a broad-spectrum antibiotic that binds reversibly to the 30S ribosomal subunit and blocks the attachment of aminoacyl-tRNA to the ribosome, thereby inhibiting protein synthesis in susceptible bacteria. It has activity against many Gram-positive and Gram-negative species, as well as some atypical bacteria. When tetracycline is added to Columbia Agar Base, the medium becomes selective due to tetracycline-susceptible organisms that are inhibited or killed, whereas strains that are intrinsically resistant or carry tetracycline-resistance determinants can still grow and form well-defined colonies on the medium.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
