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FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol, 1X Liquid Cell Culture
FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol, 1X Liquid Cell Culture
FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol, 1X Liquid Cell Culture is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- Quadruple-stage nano-filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II — Polish), reaching a 0.04 µm final cut-off
- Endotoxin release specification: < 0.05 EU/mL (USP <85> BET)
- EHAA-based Click's Medium formulation with glucose 1000 mg/L and sodium pyruvate 275 mg/L; supplied without L-glutamine or HEPES — supplement per protocol
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm)
- ISO Class 5 aseptic fill & finish; manufactured under an ISO 13485:2016 quality management system
- Mycoplasma risk controlled via 0.1 µm mycoplasma-retentive filtration (not tested per lot)
- Sodium bicarbonate-buffered (1350 mg/L); recommended atmosphere approximately 5% CO₂
- Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
- Glucose1000 mg/L (1.0 g/L)
- L-GlutamineNot present — supplement as needed
- Sodium Pyruvate275 mg/L
- HEPESNot present
- pH (USP <791>)7.4
- Osmolality (USP <785>)320 - 360 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Available sizes: 500 mL, 1000 mL.
Engineered where standard media fails
Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ is engineered to address these failure modes.
Microchannel-safe purity
0.04 µm final filter stage retains particles down to the sub-mycoplasma size range (0.2–0.3 µm); USP <788> Method 1 particulate compliance verified per lot.
Total metabolic control
Defined glucose (1.0 g/L) and sodium pyruvate (275 mg/L) levels support reproducible metabolic flux and Warburg-effect studies; custom carbon-source concentrations available on request.
Ultrapure-grade water
Prepared with Type 1 water (18.2 MΩ·cm), supporting low trace-metal and organic-carbon (TOC) background for sensitive assay systems.
Low background for imaging
Quadruple-stage filtration minimizes particulate baseline for confocal microscopy, live-cell biosensors, and TEER measurements. This formulation contains phenol red; users requiring absorbance/fluorescence-free imaging should request a phenol-red-free variant.
Rich, stable nutrient profile
Micro-batch precision manufacturing locks in amino acid and vitamin concentrations across 42 formulation components, ensuring lot-to-lot reproducibility for long-term perfusion studies.
Customization on demand
pH, glucose concentration, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol, 1X Liquid Cell Culture is processed through a four-stage serial filtration sequence reaching a 0.04 µm final cut-off — engineered to reduce mycoplasma-sized particulates, subvisible particles, and bioburden beyond what single-pass 0.22 µm filtration addresses.
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1
0.1 µm Prefiltration I
Removes large particulates and protein aggregates; protects the first 0.04 µm cartridge across microchannel-scale flow paths.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, including the mycoplasma size range (0.2–0.3 µm).
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3
0.1 µm Prefiltration II
Second dedicated prefilter protecting the second 0.04 µm cartridge; provides redundancy against upstream breakthrough.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill performed in a validated ISO Class 5 laminar-flow workstation.
Performance vs. conventional media
By reaching a 0.04 µm final pore size across four sequential stages, FluxMPS™ delivers approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration.
© Diagnocine® — DCP-CM-Q1X
Designed for next-generation cell culture platforms
FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol, 1X Liquid Cell Culture is validated for use across organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.
- Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
- Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
- Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs
Inquiry Required: The 0.01 µm MPS Grade variant is available by special order. Contact support@diagnocine.com to request it.
Micro Physiological System (MPS) & Chip
Ultra-filtered formulation supports laminar flow integrity across complex chip geometries.
Warburg Effect & Metabolic Research
Defined carbon source (1.0 g/L glucose, 275 mg/L sodium pyruvate) enables precise metabolic flux analysis and Warburg-effect studies.
iPSC-Derived Models
Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin and particulates cause off-target effects.
Endothelial & Primary Cells
Microchannel-safe purity supports endothelial barrier integrity and TEER measurements in perfusion models.
Metabolic Flux Analysis
Chemically defined base supports isotope tracing experiments. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate load supports high-content confocal imaging and optical biosensor integration.
Lot-release quality parameters
Every production lot of FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol, 1X Liquid Cell Culture undergoes the complete quality-release battery listed below before shipment.
| Parameter | Specification |
|---|---|
| Formulation | Contains: Sodium Bicarbonate, Phenol Red, Calcium, Magnesium, Glucose, Sodium Pyruvate | Without: L-Glutamine, HEPES |
| Appearance | Red, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 320 - 360 mOsm/kg H2O |
| Glucose | 1000 mg/L (1.0 g/L) |
| L-Glutamine | Not added |
| Sodium Pyruvate | 275 mg/L |
| Phenol Red | Present (11.000 mg/L) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | Compliant |
| Particulate ≥25 µm USP <788> Method 1 | Compliant |
| Water Purity | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing std. | ISO 13485:2016 ISO 13485 |
| Fill environment | ISO Class 5 (Class 100) laminar-flow workstation |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Not recommended |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Approximately 5% CO₂ (derived from sodium bicarbonate, 1350 mg/L) |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell culture / reagent grade |
| Traceability | Full lot documentation, CoA available |
| Manufacturing QMS | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch precision manufacturing |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
Total: 42 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others), organized into 3 tabs below. All ingredient names and mg/L values are reproduced from the manufacturer specification. Custom compositions available on request.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 245.530 |
| Disodium hydrogen phosphate | 7558-79-4 | 47.900 |
| Magnesium chloride anhydrous | 7786-30-3 | 164.900 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Potassium dihydrogen phosphate | 7778-77-0 | 60.000 |
| Sodium chloride | 7647-14-5 | 8000.000 |
| Sodium bicarbonate | 144-55-8 | 1350.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 30.000 |
| L-Alanine | 56-41-7 | 35.600 |
| L-Arginine hydrochloride | 1119-34-2 | 317.500 |
| L-Asparagine anhydrous | 70-47-3 | 52.800 |
| L-Aspartic acid | 56-84-8 | 53.200 |
| L-Cystine dihydrochloride | 30189-89-0 | 78.200 |
| L-Glutamic acid | 56-86-0 | 58.800 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 104.700 |
| L-Isoleucine | 73-32-5 | 130.000 |
| L-Leucine | 61-90-5 | 130.000 |
| L-Lysine hydrochloride | 657-27-2 | 181.200 |
| L-Methionine | 63-68-3 | 37.500 |
| L-Phenylalanine | 63-91-2 | 80.000 |
| L-Proline | 147-85-3 | 46.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 120.000 |
| L-Tryptophan | 73-22-3 | 25.000 |
| L-Tyrosine disodium salt dihydrate | 69847-45-6 | 118.900 |
| L-Valine | 72-18-4 | 115.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Calcium pantothenate | 137-08-6 | 2.000 |
| Choline chloride | 67-48-1 | 2.000 |
| Folic acid | 59-30-3 | 2.000 |
| Niacinamide | 98-92-0 | 2.000 |
| Pyridoxal hydrochloride | 65-22-5 | 2.000 |
| Riboflavin | 83-88-5 | 0.200 |
| Thiamine hydrochloride | 67-03-8 | 2.000 |
| OTHERS | ||
| Adenosine | 58-61-7 | 25.000 |
| Cytosine | 71-30-7 | 25.000 |
| Glucose | 50-99-7 | 1000.000 |
| Guanosine | 118-00-3 | 25.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| myo-Inositol | 87-89-8 | 4.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
| Sodium pyruvate | 113-24-6 | 275.000 |
| Uridine | 58-96-8 | 25.000 |
ISO 13485:2016 manufacturing & compliance
FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol, 1X Liquid Cell Culture is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Full quality management system certification covering manufacturing, testing, and release processes for every production lot.
Ultrapure Type 1 Water
All media prepared with 18.2 MΩ·cm resistivity Type 1 water, supporting low trace-metal and organic-carbon (TOC) background.
ISO Class 5 Fill & Finish
Aseptic filling performed in validated laminar-flow (ISO Class 5 / Class 100) workstations; 21 CFR Part 820 (QMSR) aligned.
Micro-Batch Precision
Small-batch manufacturing locks in lot-to-lot nutrient consistency critical for reproducible perfusion studies and long-term OoC experiments.
Endotoxin — USP <85> BET
Limulus Amebocyte Lysate (LAL) assay performed per manufacturing batch. Release specification: < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count test confirms ≥10 µm and ≥25 µm particulate compliance on every lot.
Osmolality — USP <785>
Freezing-point osmometry performed per USP <785>. Result: 320 - 360 mOsm/kg H2O.
Documentation — CoA & Full Lot Records
Certificate of Analysis available for every lot, including full QC panel, raw material traceability, and release signatures.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-CM-Q1X (FluxMPS™) compares
Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.
| Parameter | DCP-CM-Q1X (FluxMPS™) | Conventional Click (0.22 µm) | Standard Click alternative |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Base Formulation | Click's Medium (EHAA), w/o L-Glutamine & HEPES | Click Standard | Click Equivalent |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 stages | 1 stage | 1–2 stages |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ) | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | Variable | Variable |
| Microfluidic channel compatibility | check_circle Validated | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle On request | cancel | Limited |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Click's Medium (EHAA) Eagle Hanks' Amino Acids w/o L-Glutamine, Mercaptoethanol, 1X Liquid Cell Culture.
Supporting literature
Peer-reviewed publications supporting the scientific rationale for ultra-filtered cell culture media and microfluidic cell culture applications.
- Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
- Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
- Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
- Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
- Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
- Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
- Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
- Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
- Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
- Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175