Cell Free DNA Purification Kit for Variable Sample Volumes

Product#: NGS-cfDNA
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Cell Free DNA Purification Kit for Variable Sample Volumes

Cat.No.   NGS-cfDNA
Size    None
Store  Room temperature (15-25°C) until use.


Circulating cell-free DNA fragments, usually shorter than 1000 bp, such as tumor-specific extracellular DNA fragments in the blood or fetal nucleic acids in maternal blood can be isolated from serum or plasma.(1, 4) Analysis of circulating cell-free DNA fragments can be useful in characterizing certain cancers(3) and disease states(5) as well as in fetal genetic analysis(1, 2, 4) The concentrations of circulating cell-free DNA in biological fluids such as plasma, serum, or urine can vary considerably (~50pg/ml to >100ng/ml) and depend on the individual, disease state, therapeutic regimen, and period of gestation.(5) For example, the concentrations of circulating cell-free DNA fetal DNA was shown to be 3.4% (range 0.39%-11.9%) and 6.2% (range 2.33%-11.4%) of the total plasma DNA in early and late pregnancy, respectively.(6)

The NeoGeneStarTMCirculating DNA Kits come with proprietary NeoGeneStarTM superparamagnetic beads that have been especially designed for isolation of circulating cell-free DNA in plasma, serum or urine. Cutting edge nanotechnology fabrication methods are utilized in the GMP manufacturing facility. The NeoGeneStarTM Circulating DNA Kits enable starting sample volumes from 0.2ml to 22ml and elution volumes of 50μl; which enables concentration and enrichment of circulating cell-free DNA that is usually present at low concentrations during the early stages of pregnancy, cancer or disease state. 

Samples can be either freshly collected or frozen, though multiple freeze/thaw cycles should be avoided due to reduced yield and quality of the purified circulating cell-free DNA. The purified circulating cell-free DNA is then ready for use in subsequent analysis such as PCR, qPCR or Next Generation Sequencing (NGS) or long term frozen storage.

Solid Reagents for cfDNA Purification

Our patent pending cfDNA purification utilizes solid lysis reagents which is offers several advantages over the industry standard liquid buffers.

1)  Addition of a sample to solid reagents,  minimizes sample dilution and accomodate more starting materials, thus increasing yield of cfDNA
2)  Minimal sample dilution, maximizes cfDNA concentration and improving yield.
3)  Predispensed reagents aid lab throughput - minimized hands-on time.

Submicron SuperParaMagnetic Particles for Nucleic Acid Capture - also great for genomic DNA purification

Our superparamagnetic nanoparticles are manufactured with a unique surface chemistry which tightly and strongly preferentially binds nucleic acids over other biological molecules.  The particles together with the other components of our cfNA purification kit produce the highest quality, clean artifact free cell free nucleic acids.

Our technology consistently yields over 95% retention rate of DNA from 50 bases to greater than 2 KB and excellent binding of miRNAs and small (~20 base) DNAs for exacting analysis.

Innovative High Power Neodymium Magnets

Magnet / Sleeve designs for improved throughput by eliminating aspiration and pipetting in the work flow of particle transfer between buffers.  Designed for samples from 30uL to 200mL

MagSlide designs for low though-put labs with the need for multiple sample sizes from 50uL to 50mL.

Traditional fixed magnet designs for 96 well, 24 well, microfuge tube, 15mL & 50mL centrifuge tubes, and other formats from 50uL to 50mL.


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