DiagnoCine Precision® ELISA Kit Reagents included
- SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
- Universal Blocking Buffer: use when background interference compromises signal-to-noise ratio
- Protease Inhibitor Cocktail: incorporate during the sample preparation phase or add directly to the ELISA protocol as required to prevent protein degradation.
Exclusive additions: All kits come with three DiagnoCine Precision® reagents for improving the effectiveness of your testing and clarity of your results
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Cat Stromal Cell Derived Factor 1 (SDF-1a;CXCL12)
Cat.No.: JNB-JL58224
Size: 96T
Species: Cat
Assay Type: Sandwich
Sensitivity: 0.08ng/mL
Detection Range: 0.15-10ng/mL
Alternative Names: CXCL12; PBSF; P-BSF; SCYB12; SDF1-A; SDF1-B; SDF1A; SDF1B; TLSF-A; TLSF-B; TPAR1; Pre-B Cell Growth-Stimulating Factor; Chemokine C-X-C-Motif Ligand 12;C-X-C motif chemokine 12 cxcl12;hIRH;hSDF-1;Intercrine reduced in hepatomas;IRH;Pre-B cell growth-stimulating factor;SDF 1 beta;SDF-1;SDF-1-alpha(3-67);SDF1;SDF1_HUMAN;SDF1b;Stromal cell derived factor 1;TLSF
Research Areas: Immunology
Assay Time: 2h15m
Abbreviation: SDF-1a;CXCL12
Test Principle: This assay employs the sandwich enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Cat SDF-1a;CXCL12. Add Sample, Standard, and HRP-antibody by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is positive to the Cat SDF-1a;CXCL12 in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. You can calculate the concentration of Cat SDF-1a;CXCL12 in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Cat SDF-1a;CXCL12 in samples. No significant cross-reactivity or interference between Cat SDF-1a;CXCL12 and analogues was observed.
Recovery rate: 80%-120%
Precision: Intra-CV and Inter-CV <10%
Shipping: Ice Bag
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