Ready to use
Cat Interleukin 2, IL-2 ELISA Kit
Cat. No#: BTL-E0051Cat
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: ,96T
Sensitivity: 0.25ng/L
Detection range: 0.5-150ng/L
Sample type: Serum, plasma, cell culture supernates
Reactive with: Cat
Product overview
Full product name
Interleukin 2
SKU#.: ? BTL- |
E0051Cat |
Assay type |
Sandwich |
Size |
0.25ng/L |
Detection range |
0.5-150ng/L |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Cat |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. |
UniProt accession |
17,654 |
GeneID |
751114 |
Synonyms |
IL 2;IL2;IL-2;Interleukin-2;T-cell growth factor;TCGF |
Gene names |
IL2 |
Research area |
Immunology |
Target protein |
IL-2 |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0051Cat are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 80ng/L | 2.193 | 2.308 | 2.271 |
| 2.423 | |||
| 40ng/L | 1.246 | 1.233 | 1.196 |
| 1.22 | |||
| 20ng/L | 0.725 | 0.757 | 0.72 |
| 0.79 | |||
| 10ng/L | 0.382 | 0.353 | 0.315 |
| 0.323 | |||
| 5ng/L | 0.19 | 0.188 | 0.151 |
| 0.186 | |||
| 0ng/L | 0.029 | 0.037 | 0 |
| 0.046 |
Precision
We measured random samples of E0051Cat within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 25.05 | 1.36 | 5.45 |
| Intra-Assay | 2 | 18 | 55.24 | 1.58 | 2.86 |
| Intra-Assay | 3 | 18 | 10.54 | 0.69 | 6.52 |
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