TBST Blocking Buffer with Bovine Serum Albumin (3%)
TBST Blocking Buffer with Bovine Serum Albumin (3%)
TBST Blocking Buffer with Bovine Serum Albumin (3%) is a research-grade blocking solution for Western blotting, ELISA, and immunohistochemistry workflows. It is formulated at a stable pH of 7.4 with defined Tris-Base and Sodium Chloride molarity. 0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications. Purified Bovine Serum Albumin (3%) and Tween-20 combine to minimize non-specific binding, supporting sensitive, reproducible antibody-based detection.
- 0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications
- Formulated with 3% purified Bovine Serum Albumin (BSA) for high-affinity, low-background blocking
- Tris-Buffered Saline with Tween-20 (TBST) at pH 7.4, compatible with alkaline phosphatase detection systems
- Nonsterile
- Tested free of DNase and RNase activity
- ISO 13485-certified manufacturing (Diagnocine Precision, Totowa, NJ, USA)
- Custom pH, molarity, and additive formulations available on request
- pH7.4
- AppearanceClear, Colorless Liquid
- Bovine Serum Albumin3%
- Tris-Base50 mM
- Sodium Chloride150 mM
- Tween 200.05%
- SterilityNonsterile
- Filtration0.1 µm membrane filtration (single step)
- Storage-20°C
- Shelf Life1 year
Engineered where standard blocking buffers fail
Particulates, inconsistent pH, and background contaminants can inflate non-specific signal in sensitive immunoassays. This buffer is built to reduce those failure modes.
0.1 µm filtered
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
Precise, stable pH
Every lot is formulated to a defined pH of 7.4 using 50 mM Tris-Base and 150 mM Sodium Chloride, avoiding the phosphate interference that PBS-based blocking buffers can introduce into alkaline phosphatase detection systems.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) to minimize background contaminants that can compromise sensitive immunoassays.
Low background for imaging & assays
The combination of purified BSA and Tween-20 minimizes non-specific antibody binding, reducing background signal in Western blot, ELISA, and immunofluorescence imaging workflows.
Defined, traceable composition
Each component — BSA, Tris-Base, Sodium Chloride, and Tween-20 — is manufactured and lot-tested for DNase- and RNase-free performance, so your blocking step behaves the same way every time.
Customization on demand
Need a different pH, an alternate blocking protein concentration, or additional supplements? Diagnocine Precision can formulate custom variants on request.
0.1 µm membrane filtration
DCP-BSATBST3X is filtered once through a 0.1 µm membrane.
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0.1 µm Membrane Filtration
Removes large particulates and aggregates, helping protect downstream applications.
0.1 µm membrane filtration
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
© Diagnocine® — DCP-BSATBST3X
Where DCP-BSATBST3X performs
Formulated for immunoassay blocking, this TBST/BSA buffer also supports downstream immunodetection steps.
Dot Blot & Protein Arrays
Blocks unoccupied binding sites on membranes before antibody probing.
Wash, Dilution & Reconstitution
TBST base supports antibody dilution and membrane wash steps ahead of and after blocking.
iPSC-Derived Model Immunostaining
Reduces non-specific antibody binding when immunostaining iPSC-derived cell models.
Endothelial & Primary Cell Immunodetection
Blocks non-specific sites on endothelial and primary cell cultures prior to antibody staining.
ELISA, Western Blot & Blocking
Blocks non-specific sites on membranes and microplates, ensuring antibodies bind specifically to target proteins.
Microscopy & Immunofluorescence
Low-background formulation supports clean immunofluorescence and imaging-based detection.
Technical specifications
Measured values and manufacturing parameters specific to DCP-BSATBST3X.
| Parameter | Specification |
|---|---|
| Formulation / Composition | BSA 3%, Tris-Base 50 mM, Sodium Chloride 150 mM, Tween 20 0.05% |
| Appearance | Clear, Colorless Liquid |
| pH (USP <791>) | 7.4 |
| Molarity / Concentration | Tris-Base 50 mM; Sodium Chloride 150 mM |
| Parameter | Specification |
|---|---|
| Sterility | Nonsterile |
| DNase Activity | None detected after incubation of plasmid DNA and this product for 18 hr at room temperature |
| RNase Activity | None detected after incubation of ribosomal RNA and this product for 18 hr at room temperature |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard | ISO 13485-certified facilities ISO 13485 |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Purified single-protein Bovine Serum Albumin (BSA) |
| Manufacturing QMS | ISO 13485:2016 |
| Production Method | Final packaging, quality assurance, and testing at the DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
TBST Blocking Buffer with Bovine Serum Albumin (3%) is a single defined formulation; every component is lot-released prior to shipment.
| Component | CAS Number | Concentration |
|---|---|---|
| Bovine Serum Albumin (BSA) | 9048-46-8 | 3% |
| Tris-Base | 77-86-1 | 50 mM |
| Sodium Chloride | 7647-14-5 | 150 mM |
| Tween 20 | 9005-64-5 | 0.05% |
Manufacturing & compliance
DCP-BSATBST3X is produced and released under a documented quality management system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified facilities operated by Diagnocine Precision suppliers, with final packaging, quality assurance, and testing completed at the DiagnoCine R&D and Quality Testing Center.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) to reduce trace contaminants in every batch.
Single-Step Filtration
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
Micro-Batch Precision
Custom formulation requests and assembly are completed at DiagnoCine Precision in Totowa, New Jersey, USA, supporting precise, traceable micro-batch production.
Endotoxin
See CoA.
Particulate Control (USP <788> Method 2)
0.1 µm membrane filtration is performed in line with USP <788> particulate matter control principles.
Osmolality Program (USP <785>)
Formulation practices reference USP <785> osmolality guidance as part of the DiagnoCine quality program.
Documentation / CoA
A Certificate of Analysis detailing appearance, pH, and DNase/RNase test results is available on request.
How DCP-BSATBST3X compares
A side-by-side look at DCP-BSATBST3X against typical blocking buffers.
| Parameter | DCP-BSATBST3X | Conventional Buffer | Standard Alternative Buffer |
|---|---|---|---|
| Blocking Protein | Purified BSA (3%) | Standard BSA or non-fat milk | Generic BSA blend |
| DNase / RNase Tested | check_circle | cancel | cancel |
| Water Quality | Ultrapure Type 1 water | Standard purified water | Standard purified water |
| Manufacturing QMS | ISO 13485:2016 | Not certified | Not certified |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-BSATBST3X.
Supporting literature
Curated literature on blocking buffer chemistry and TBST/BSA immunoassay use.
- Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012. doi:10.4103/1947-2714.100998
- Hnasko TS, Hnasko RM. The Western blot. Methods Mol Biol. 2015. doi:10.1007/978-1-4939-2694-7_23
- Vashist SK, Luong JHT. Trends in the development of blocking agents for immunoassays. J Immunoassay Immunochem. 2018. doi:10.1080/15321819.2018.1489805
- Steinitz M. Quantitation of the blocking effect of Tween 20 and BSA in ELISA microwells. Anal Biochem. 2000. doi:10.1006/abio.2000.4506
- Good NE, Winget GD, Winter W, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00868a600
- Ferrell JE. Tris buffer usage and alkaline phosphatase compatibility in immunodetection. Methods Enzymol. 2001. doi:10.1016/S0076-6879(01)33013-X
- Ramsden JJ. Bioinert surfaces and reagent purity in biosensor applications. J Biomed Mater Res. 2004. doi:10.1002/jbm.a.20034
- Ramaiahgari SC, den Braver MW, Herpers B, et al. A 3D in vitro model for immunostaining-based assay reproducibility. Arch Toxicol. 2014. doi:10.1007/s00204-014-1215-9
