TBS Blocking Buffer with Bovine Serum Albumin (3%)
Cat No.: DCP-BSATBS3X
Size: 100 mL, 500 mL & 1 L
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
TBS Blocking Buffer with Bovine Serum Albumin (3%) is a specialized reagent used in various biochemical and molecular biology applications, particularly in immunoassays such as Western blotting and ELISA. This blocking buffer is designed to minimize non-specific binding, thereby enhancing the sensitivity and specificity of these assays. Bovine Serum Albumin (BSA) serves as the primary blocking agent. Tris-Buffered Saline (TBS) provides a stable pH environment, which is crucial for maintaining the integrity of proteins and antibodies during assays. TBS does not contain phosphate, which can interfere with alkaline phosphatase activity, making it a preferred choice for such applications. So, it is particularly beneficial for assays involving alkaline phosphatase detection systems.
Applications:
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Western Blotting: Used to block non-specific sites on membranes, ensuring that antibodies bind only to target proteins.
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ELISA (Enzyme-Linked Immunosorbent Assay): Reduces background noise, improving the accuracy of antigen-antibody interactions.
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Immunohistochemistry (IHC): Prevents non-specific binding of antibodies to tissue sections, enhancing the clarity of staining.
Advantages:
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High Affinity for Proteins: BSA is a purified single-type protein, which reduces the risk of cross-reactivity and provides a consistent blocking effect.
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Compatibility with Phosphorylated Targets: BSA in TBS is particularly recommended for assays involving phosphorylated proteins or when using alkaline phosphatase-based detection methods, as it avoids issues associated with other blocking agents like non-fat dry milk, which can interfere with these targets.
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Reduced Background Interference: The use of BSA helps to minimize background signals, leading to clearer and more reliable results.
Composition:
| Ingredients |
Concentration |
| BSA |
3% |
| Tris-Base |
50 mM |
| Sodium Chloride |
150 mM |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 7.4
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Storage: -20 ℃ (avoid repeated freeze-thaw cycles to prevent protein degradation)
- Shipping: Room temperature or blue ice
- Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
