PBS Blocking Buffer with Bovine Serum Albumin (3%)
Cat No.: DCP-BSAPBS3X
Size: 100 mL, 500 mL & 1 L
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
PBS Blocking Buffer with Bovine Serum Albumin (3%) is a widely used reagent in immunoassays such as Western blotting, ELISA, and immunohistochemistry. It serves to minimize non-specific binding of antibodies to the solid phase, thereby reducing background noise and enhancing the signal-to-noise ratio in assays. BSA is a stable, soluble protein that effectively covers potential non-specific binding sites on assay surfaces. The primary function of this buffer is to block non-specific sites on membranes or microplates, preventing antibodies or other detection reagents from binding to these sites. This ensures that the antibodies specifically bind to the target proteins or antigens, improving assay sensitivity and accuracy.
* Not Compatible with Lectin Probes: BSA contains carbohydrates that can increase background when using lectin probes, so it may not be suitable for all applications
Applications
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Western Blotting: Used to block membranes and reduce non-specific binding of antibodies, improving the signal-to-noise ratio.
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ELISA: BSA blocking buffer is used to block microplates, preventing non-specific interactions in enzyme-linked immunosorbent assays.
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Immunohistochemistry: Helps in blocking tissue sections to reduce background staining.
Advantages:
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Compatibility: BSA is compatible with most detection systems and antibodies, making it a versatile choice for various applications.
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Reduced Background: By blocking non-specific sites, BSA helps reduce background noise, leading to clearer and more reliable results.
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Phosphoprotein Assays: BSA is particularly useful in assays involving phosphorylated targets because it does not contain phosphoproteins, unlike nonfat dry milk, which can interfere with such assays.
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Biotin-Free: It is suitable for use in biotin-avidin detection systems as it does not contain biotin, which could otherwise interfere with the assay.
Composition:
| Ingredients |
Concentration |
| BSA |
3% |
| Sodium Chloride |
137 mM |
| Potassium Chloride |
2.7 mM |
| Sodium Phosphate dibasic |
10 mM |
| Potassium Phosphate monobasic |
1.8 mM |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 7.4
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: -20 ℃
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Shelf life: 1 year
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
