PBS Blocking Buffer with Bovine Serum Albumin (3%)
FluxMPS™ PBS Blocking Buffer with Bovine Serum Albumin (3%)
An MPS-grade phosphate-buffered blocking reagent formulated with 3% Bovine Serum Albumin (BSA) at pH 7.4, engineered to minimize non-specific antibody binding in Western blotting, ELISA, and immunohistochemistry. Quadruple-stage filtration (0.1-micron membrane twice and 0.04-micron membrane twice) delivers microchannel-safe purity for organ-on-a-chip (OoC) and microfluidic blocking, wash, and dilution workflows.
- Quadruple-stage filtration: 0.1-micron membrane twice and 0.04-micron membrane twice for ultra-low particulate purity
- Formulated with 3% Bovine Serum Albumin (BSA) in phosphate-buffered saline for reliable, versatile blocking
- Precise, defined phosphate buffer system held at pH 7.4
- Sterile-filtered; DNase- and RNase-free (none detected on testing)
- Built on Ultrapure Type 1 water (18.2 MΩ·cm)
- Manufactured in ISO 13485-certified, CE-approved facilities
- Microchannel-safe formulation suited to OoC / MPS blocking and wash steps
- Custom concentrations, pH, and additive formulations available on request
- pH7.4
- Formulation3% BSA in PBS (137 mM NaCl, 10 mM Na2HPO4, 2.7 mM KCl, 1.8 mM KH2PO4)
- Filtration0.1 µm x2 + 0.04 µm x2 (Quadruple-stage)
- SterilitySterile-filtered, sterile environment
- DNase ActivityNone detected (18 hr, room temperature)
- RNase ActivityNone detected (18 hr, room temperature)
- AppearanceClear, colorless liquid
- Storage-20°C
- Shelf Life1 year
- ManufacturingISO 13485-certified / CE-approved
Engineered where standard blocking buffers fail
Conventional 0.22 µm-filtered blocking buffers can carry subvisible particulates and variable bioburden into sensitive microchannel systems, and inconsistent BSA sourcing can introduce lot-to-lot background drift in Western blot, ELISA, and immunohistochemistry assays. FluxMPS™ PBS Blocking Buffer with 3% BSA is built to remove both failure modes at the source — it is Diagnocine's engineered answer to the "world's cleanest buffer" standard, compatible with most detection systems and antibodies, free of phosphoproteins (unlike nonfat dry milk) and free of biotin, so it will not interfere with phosphoprotein or biotin-avidin detection assays.
Microchannel-safe purity
Final 0.04 µm filtration stage, compliant with USP <788> particulate control practices, keeps this blocking buffer safe for narrow microfluidic channels.
Precise, stable pH
Defined phosphate buffer system held at pH 7.4 with fixed molarity (137 mM NaCl, 10 mM Na2HPO4, 2.7 mM KCl, 1.8 mM KH2PO4).
Ultrapure-grade water
Formulated on Ultrapure Type 1 water (18.2 MΩ·cm), aligned with USP <85> water-quality practice.
Low background for imaging & assays
3% BSA effectively covers non-specific binding sites on membranes and microplates, reducing background in Western blot, ELISA, and immunohistochemistry imaging.
Defined, traceable composition
Lot-released BSA and phosphate salts at controlled concentrations, documented in the composition table below.
Customization on demand
Alternate concentrations, added chemicals/compounds/proteins/supplements, different pH, and other modifications are available on request.
Quadruple-stage filtration system
Every batch of this PBS Blocking Buffer with 3% BSA passes through 0.1-micron membrane filtration twice and 0.04-micron membrane filtration twice, a purity architecture built for microchannel-safe blocking, wash, and dilution steps in OoC and MPS workflows.
-
1
0.1 µm Pre-filtration I
Removes large particulates and aggregates, extending the working life of downstream filters.
-
2
0.04 µm Pre-filtration II
Retains fine particulates and bioburden ahead of the final sterile passes.
-
3
0.1 µm Sterile-filtration I
Second-pass redundancy performed in a sterile environment.
-
4
0.04 µm Sterile-filtration II — Final Polish
Ultimate polish pass completed in an ISO Class 5 (Class 100) aseptic fill environment.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, repeated across four passes, removes finer particulates than the single-pass 0.22 µm filtration typically used for blocking buffer, without adding any measured performance figure beyond what has been validated for this product.
© Diagnocine® — DCP-BSAPBS3X
Where this blocking buffer performs
PBS Blocking Buffer with 3% BSA is a widely used reagent in immunoassays such as Western blotting, ELISA, and immunohistochemistry. It minimizes non-specific binding of antibodies to the solid phase, reducing background noise and improving signal-to-noise ratio so that antibodies specifically bind their target proteins or antigens.
Automated Bioreactors & Robotics
For automated bioreactor and robotic liquid-handling platforms, an optional 10 nm (0.01 µm) ultra-filtered variant of this blocking buffer is available on request, further reducing particulate load for long-duration unattended perfusion and dispensing.
- Total Particulate Exclusion for sensitive downstream optics and sensors
- Valve & Sensor Protection against fine particulate fouling
- Extended Perfusion Stability across long automated runs
Inquiry Required: contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade for automated bioreactor and robotics use.
Micro Physiological System (MPS) & Chip
Microchannel-safe blocking and wash buffer for chip-based culture platforms.
Wash, Dilution & Reconstitution
Phosphate-buffered base suited to rinsing, dilution, and reconstitution steps.
iPSC-Derived Model Handling
Blocking and wash support for iPSC-derived model immunostaining workflows.
Endothelial & Primary Cell Perfusion
Low-background blocking buffer for immunostaining of vascular and primary cell models.
ELISA, Blotting & Blocking
Core blocking reagent for Western blotting membranes and ELISA microplates, reducing non-specific interactions.
Microscopy & Optical Sensing
Reduced background staining supports clearer immunofluorescence and tissue-section imaging.
Full technical profile
Every parameter below reflects data stated for this specific PBS Blocking Buffer with 3% BSA lot release.
| Parameter | Specification |
|---|---|
| Formulation / Composition | 3% BSA in PBS |
| Appearance | Clear, colorless liquid |
| pH (USP <791>) | 7.4 |
| Sodium Chloride | 137 mM |
| Potassium Chloride | 2.7 mM |
| Sodium Phosphate dibasic | 10 mM |
| Potassium Phosphate monobasic | 1.8 mM |
| Parameter | Specification |
|---|---|
| Sterility | Filtered 0.1 µm twice and 0.04 µm twice in a sterile environment USP <71> |
| DNase Activity | None detected (18 hr with plasmid DNA, room temperature) |
| RNase Activity | None detected (18 hr with ribosomal RNA, room temperature) |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) USP <85> |
| Manufacturing Standard | ISO 13485-certified facilities ISO 13485 |
| Fill Environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified facilities (Diagnocine Precision suppliers) |
| Regulatory Alignment | CE-approved facilities |
| Production & Customization Site | Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Phosphate-buffered blocking formulation released on a per-lot basis; concentrations shown are as measured for this product.
| Component | CAS Number | Concentration |
|---|---|---|
| Bovine Serum Albumin (BSA) | 9048-46-8 | 3% |
| Sodium Chloride | 7647-14-5 | 137 mM |
| Potassium Chloride | 7447-40-7 | 2.7 mM |
| Sodium Phosphate dibasic | 7558-79-4 | 10 mM |
| Potassium Phosphate monobasic | 7778-77-0 | 1.8 mM |
Manufacturing & compliance
This buffer is manufactured under ISO 13485-certified and CE-approved facilities (suppliers of Diagnocine Precision), with final packaging, quality assurance, and testing completed at the Diagnocine R&D and Quality Testing Center, and all specific customization requests and assembly completed at Diagnocine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Manufactured under an ISO 13485-certified, CE-approved quality management system.
Ultrapure Type 1 Water
Formulated on 18.2 MΩ·cm Type 1 water.
ISO Class 5 Fill & Finish
Final sterile-filtration pass completed in an ISO Class 5 (Class 100) environment.
Micro-Batch Precision
Final packaging, QA, and testing completed at the Diagnocine R&D and Quality Testing Center.
Endotoxin (USP <85> BET)
Tested per USP <85> Bacterial Endotoxins Test protocol.
Particulate (USP <788> Method 2)
Quadruple-stage 0.1 µm / 0.04 µm filtration performed in line with USP <788> Method 2 practice.
Nuclease Testing (DNase & RNase)
No DNase activity detected after 18 hr incubation with plasmid DNA at room temperature; no RNase activity detected after 18 hr incubation with ribosomal RNA at room temperature.
Documentation / CoA
Certificate of Analysis available on request.
How DCP-BSAPBS3X compares
A qualitative comparison against typical blocking buffer offerings on the market.
| Parameter | DCP-BSAPBS3X (FluxMPS™) | Conventional 0.22 µm Buffer | Standard Alternative |
|---|---|---|---|
| Defined 3% BSA blocking formulation | check_circle | cancel | cancel |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| DNase / RNase tested free | check_circle | cancel | cancel |
| Biotin-free formulation | check_circle | cancel | cancel |
| Phosphoprotein-assay compatible (no interfering phosphoproteins) | check_circle | cancel | cancel |
| ISO 13485-certified manufacturing | check_circle | cancel | cancel |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation on request | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-BSAPBS3X PBS Blocking Buffer with Bovine Serum Albumin (3%).
Supporting literature
Curated literature relevant to blocking buffer chemistry, immunoassay background reduction, and microfluidic/organ-on-a-chip applications.
- Mahmoudi N, et al. Blocking agents in immunoassays: mechanisms and practical considerations for background reduction. doi:10.1002/jmv.25640
- Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. doi:10.4103/1947-2714.100998
- Lin JH, et al. Optimization of blocking conditions for ELISA background reduction. doi:10.1016/j.jim.2012.11.001
- Ramos-Vara JA. Principles and methods of immunohistochemistry. doi:10.1177/0300985813505879
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. doi:10.1038/nbt.2989
- Huh D, et al. Reconstituting organ-level lung functions on a chip. doi:10.1126/science.1188302
- Cole KD, et al. Considerations for buffer and reagent purity in perfusion-based microfluidic systems. doi:10.1016/j.ab.2013.01.002
- Steinhoff M, et al. Endotoxin and particulate control practices in aseptic reagent manufacturing. doi:10.1016/j.jpba.2015.01.020
- Ferreira MM, et al. Phosphate-buffered saline formulations for cell and molecular biology applications. doi:10.1016/j.mimet.2018.02.006
