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Bovine Major Prion Protein, PRNP ELISA Kit
Product#: BTL-E2230Bo
$686.40
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Bovine Major Prion Protein, PRNP ELISA Kit
Cat. No#: BTL-E2230Bo
*All types, size 48T, of ELISA kits are available, including special MOQ value pricing. For more details, please contact us at support@diagnocine.com
Product Summary
Size: 96T,
Sensitivity: 0.021ng/ml
Detection range: 0.05-20ng/ml
Sample type: Serum, plasma, cell culture supernates
Reactive with: Bovine
Product overview
Full product name
Major Prion Protein
SKU#.: ? BTL- |
E2230Bo |
Assay type |
Sandwich |
Size |
0.021ng/ml |
Detection range |
0.05-20ng/ml |
Sample type |
Serum, plasma, cell culture supernates |
Species |
Bovine |
Storage |
2-8ºC |
Assay time |
1h 30m |
Background |
Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains (By similarity). |
UniProt accession |
28,614 |
GeneID |
281427 |
Synonyms |
CD antigen CD230;Major prion protein;Major scrapie-associated fibril protein 1;PRNP;PrP |
Gene names |
PRNP |
Research area |
Neuroscience |
Target protein |
PRNP |
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2230Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 12ng/ml | 2.324 | 2.183 | 2.141 |
| 2.042 | |||
| 6ng/ml | 1.184 | 1.184 | 1.142 |
| 1.185 | |||
| 3ng/ml | 0.86 | 0.804 | 0.761 |
| 0.747 | |||
| 1.5ng/ml | 0.506 | 0.503 | 0.461 |
| 0.501 | |||
| 0.75ng/ml | 0.252 | 0.255 | 0.213 |
| 0.258 | |||
| 0ng/ml | 0.055 | 0.042 | 0 |
| 0.03 |
Precision
We measured random samples of E2230Bo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 6.9 | 0.25 | 3.6 |
| Intra-Assay | 2 | 18 | 4.3 | 0.31 | 7.2 |
| Intra-Assay | 3 | 18 | 1.8 | 0.14 | 7.6 |