Blocking Solution Trial Set, Western Blot (Size: 4 X 20ml)

Product#: BCL-BKSW-01
$54.00
Availability:
Ships in 1-2 weeks

Blocking Solution Trial Set, Western Blot 

Cat. No.  BCL-BKSW-01
Size 1 Set (4 X 20ml)
Storage  4°C

Description

Blocking agent. 100% chemical components. Good for WB and antibody-detecting ELISA.

Finding the appropriate blocking solution is an important factor to get a best data in Western Blot and ELISA. 
Four different types of blocking solutions : c-Block / h-Block / k-Block / b-Block make this works easy. 
Moreover, the blocking solutions for ELISA are desinged to long-term storage of pre-coated plate.

b-Block / h-Block / k-Block / c-Block Blocking Solutions for Western Blot and ELISA

Bloking-Solution_fig1.png 

Features

  • Ready-to-Use
  • Optimized blocking solution for Western Blot and ELISA
  • Pre-coated ELISA plate can be stored for several months.
  • b-Block : BSA-based blocking solution. Good for various applications. (Not available now)
  • h-Block : Casein-based blocking solution. Good for various applications. (Not available now)
  • k-Block : Degraded casein-based blocking solution. It shows consistent performance in various applications. (Not available now)
  • c-Block : 100% chemically defined components. Protein free. Best for Western Blot and antibody-detecting ELISA.
  • Trial set : Composed of all the above four solutions. Good for screening of most suitable blocking solution in your work. (C- Block only) 
---Last updated, May 11, 2021--- 
 
Recommendations for screening best blocking solution
For Western Blot
  • To get higher signal (accept some background increase) : k-Block or c-Block
  • To reduce background (accept some decrease of signal) : b-Block or h-Block
  • Using as a standard blocking : h-Block or b-Block
  • Detecting a phospho-protein : c-Block or b-Block
For ELISA
  • Antigen-detecting ELISA - Direct method : c-Block , b-Block or k-Block 
    • Sandwich method : k-Block , b-Block or h-Block
  • Antibody-detecting ELISA
    • Direct method (detect antibody captured by pre-coated antigen) : c-Block , b-Block or k-Block 
    • Sandwich method : k-Block , b-Block or h-Block
Data
Blocking-Solution_fig2.png 
Fig. 1 Comparison of blocking solutions M : Marker / SM : 5% Skim Milk / kB : k-Block / hB : h-Block / bB : b-Block / cB : c-Block


Blocking-Solution_fig3.png 
Fig. 2 Sandwich ELISA Data


Blocking-Solution_fig4.png 
Fig. 3 Stability data of pre-coated plate
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