FluxMPS™ Blasticidin: 1000X Solution, 5x10 mL
A ready-to-use 1000X Blasticidin S HCl selection antibiotic solution at 10 mg/mL, filtered through a 0.1 µm membrane for particulate and contaminant control. Supplied as five single-use 10 mL aliquots to minimize freeze/thaw stress on stock solutions used across standard, microfluidic, and organ-on-a-chip (OoC) cell culture workflows.
- 1000X liquid stock solution at 10 mg/mL Blasticidin S hydrochloride, ready for direct dilution into growth medium
- Selection agent for cells expressing bsr, bsd, or BSD resistance genes
- Filtered through a 0.1 µm membrane for particulate and contaminant control (single-stage process)
- Supplied as 5 x 10 mL single-use aliquots (50 mL total) to minimize repeated freeze/thaw cycles
- Compatible with CRISPR-Cas9 workflows as a selectable marker for isolating gene-edited cells
- Molecular formula C17H26N8O5·HCl; molecular weight 458.9 g/mol; PubChem CID 356629; UN 2811
- Manufactured under an ISO 13485:2016 quality management system; final QC, customization, and packaging performed at Diagnocine, Totowa, NJ
- Store at -20°C protected from light; avoid frost-free freezers and repeated freeze/thaw cycles; 9-month shelf life from date of manufacture
- Active CompoundBlasticidin S Hydrochloride
- Concentration10 mg/mL (1000X)
- Molecular FormulaC17H26N8O5·HCl
- Molecular Weight458.9 g/mol
- Filtration0.1 µm membrane (single-stage)
- Pack Size5 x 10 mL (50 mL total)
- Storage-20°C, protect from light; avoid frost-free freezers
- Freeze-ThawAvoid multiple freeze/thaw cycles
- Shelf Life9 months from date of manufacture, unopened
- HandlingHarmful — use fume hood and PPE
Engineered for reliable, reproducible selection
Standard antibiotic stock solutions are often prepared without membrane filtration and undergo repeated freeze/thaw cycling on the bench, both of which can introduce particulate contamination and reduce lot-to-lot consistency. DCP-10BSH1000X_50mL addresses both failure modes with a filtered, single-use aliquot format.
Membrane-filtered purity
Filtered through a 0.1 µm membrane to reduce particulate and microbial contaminants before fill.
Precise, ready-to-use potency
Supplied at a defined 10 mg/mL (1000X) concentration for consistent dilution and dosing across experiments.
Single-use aliquot format
5 x 10 mL aliquots limit each vial to a single working use, avoiding the freeze/thaw cycling that degrades stock solutions.
Traceable identity
Documented PubChem CID, UN transport number, molecular formula, and molecular weight support full chain-of-custody records.
Validated selection performance
Selects against cells lacking bsr, bsd, or BSD resistance genes, supporting stable line generation and gene-editing workflows.
Customization on demand
Concentration, fill volume, and packaging can be customized — contact support@diagnocine.com.
Filtration
DCP-10BSH1000X_50mL is processed through a single 0.1 µm membrane filtration step to reduce particulate and microbial contaminants prior to aseptic fill. This is a single-stage filtration process.
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0.1 µm Filtration
Removes particulate matter and large contaminants from the formulated antibiotic solution prior to fill.
Formulation facts
DCP-10BSH1000X_50mL is filtered once through a 0.1 µm membrane and packaged as single-use aliquots to limit contamination risk from repeated handling.
Selection, editing, and contamination control
Blasticidin S HCl is a nucleoside antibiotic that inhibits protein synthesis in both prokaryotic and eukaryotic cells, making it a versatile selection agent across standard, microfluidic, and organ-on-a-chip (OoC) cell culture workflows.
Resistance Gene Selection
Selects for cells stably expressing the bsr, bsd, or BSD resistance genes following transfection or transduction.
Contamination Control
Inhibits growth of susceptible microbial contaminants in cell culture, helping protect valuable cultures.
Protein Synthesis Research
A well-characterized inhibitor of peptide bond formation and peptidyl-tRNA hydrolysis, useful for studying translation mechanisms.
CRISPR-Cas9 Selectable Marker
Used to identify and isolate cells that have successfully undergone CRISPR-Cas9 gene editing.
Antimicrobial Susceptibility Testing
Supports panel-, disc-, and MIC strip-based antimicrobial susceptibility tests (ASTs) against gram-positive and gram-negative organisms.
Microphysiological System (MPS) Selection
Applicable as a selection reagent within microfluidic, organ-on-a-chip (OoC), and microphysiological system (MPS) culture protocols.
Technical specifications
Key physical, chemical, safety, and regulatory parameters for DCP-10BSH1000X_50mL.
| Parameter | Specification |
|---|---|
| Active Compound | Blasticidin S Hydrochloride |
| Concentration | 10 mg/mL (1000X) |
| Molecular Formula | C17H26N8O5·HCl |
| Molecular Weight | 458.9 g/mol |
| PubChem CID | 356629 |
| Formulation | Liquid solution |
| Parameter | Specification |
|---|---|
| Filtration Process | 0.1 µm membrane filtration (single-stage) |
| UN Transport Number | UN 2811 |
| Hazard Handling | Harmful — use fume hood and PPE |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C, protect from light |
| Freeze-Thaw | Avoid multiple freeze/thaw cycles; do not store in a frost-free freezer |
| Shelf Life | 9 months from date of manufacture, unopened |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory Alignment | Research Use Only (RUO); ISO 13485:2016 aligned manufacturing |
| Production & Customization | Final QC, customization, and packaging at Diagnocine, Totowa, NJ, USA |
| Intended Use | Research Use Only — cell culture selection agent |
Compound identity & composition
DCP-10BSH1000X_50mL is a single-component antibiotic solution. The tables below list the active compound and its physicochemical and regulatory identifiers.
| Component | CAS Number | Concentration |
|---|---|---|
| ACTIVE PHARMACEUTICAL INGREDIENT | ||
| Blasticidin S Hydrochloride | 3055-99-0 | 10 mg/mL (1000X) |
| Parameter | Value |
|---|---|
| PHYSICOCHEMICAL PROPERTIES | |
| Molecular Formula | C17H26N8O5·HCl |
| Molecular Weight | 458.9 g/mol |
| Identifier | Value |
|---|---|
| IDENTIFIERS & REGULATORY | |
| PubChem CID | 356629 |
| UN Transport Number | UN 2811 |
Manufacturing & compliance
DCP-10BSH1000X_50mL is manufactured, filtered, and packaged under a controlled quality system, with final release testing and customization performed at Diagnocine.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers under a controlled quality management system.
0.1 µm Membrane Filtration
Single-stage 0.1 µm membrane filtration reduces particulate and microbial contaminants before fill.
Per-Lot QC & Documentation
Final packaging, quality assurance, and testing are performed at the Diagnocine R&D and Quality Testing Center.
Finished in Totowa, NJ
All specific customization requests and assembly are accomplished at Diagnocine Precision in Totowa, New Jersey, USA.
Manufacturing Standard
ISO 13485:2016 certified manufacturing; Research Use Only (RUO) labeling.
Transport Classification
UN 2811 — observe applicable shipping and handling regulations.
Documentation
A Certificate of Analysis (CoA) is available on request.
Handling & Safety
Blasticidin is a harmful compound. Use a fume hood and appropriate personal protective equipment; do not ingest or inhale.
How DCP-10BSH1000X_50mL compares
A comparison of filtration, format, and documented specifications against unfiltered stock preparations and published supplier data for related cell culture reagents.
| Parameter | DCP-10BSH1000X_50mL (FluxMPS™) | Other Suppliers / Alternatives |
|---|---|---|
| 0.1 µm Membrane Filtration | check_circle Yes | cancel Not specified |
| Single-Use Aliquot Format (5 x 10 mL) | check_circle Yes | cancel Not specified |
| Custom Formulation Available | check_circle Yes | cancel Not specified |
| ISO 13485:2016 Manufacturing | check_circle Yes | cancel Not specified |
| CAS & PubChem Traceability Documented | check_circle Yes | cancel Not specified |
| Endotoxin (release specification) | FluxMPS™ — Not specified (not a cell culture growth medium) | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about using DCP-10BSH1000X_50mL for cell culture selection.
Supporting literature
Peer-reviewed literature supporting the mechanism, selection use, and research applications of Blasticidin S HCl.
- Takeuchi T, Hirayama K, Ueda K, Sakumoto H, Tomita R. Blasticidin S, a new antibiotic. J Antibiot. 1958. doi:10.11554/antibioticsa.11.1_1
- Yamaguchi H, Yamamoto C, Tanaka N. Inhibition of protein synthesis by blasticidin S. J Biochem. 1965;57:667-677. doi:10.1093/oxfordjournals.jbchem.a129062
- Izumi M, Miyazawa H, Kamakura T, Yamaguchi I, Endo T, Hanaoka F. Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells. Exp Cell Res. 1991;197:229-233. doi:10.1016/0014-4827(91)90427-V
- Kimura M, Takatsuki A, Yamaguchi I. Blasticidin S deaminase gene from Aspergillus terreus (bsr): molecular cloning and its use as a selectable marker for transfection. Biochim Biophys Acta. 1994;1219:653-659. doi:10.1016/0167-4781(94)90223-2
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
- Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013;8:2281-2308. doi:10.1038/nprot.2013.143
- Clinical and Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard M07. clsi.org/m07
- Sasaki H, Nishikawa S, Kobayashi T. Antibiotic resistance markers in stable cell line generation. Methods Mol Biol. 2016;1373:1-12. doi:10.1007/7651_2015_269
- Kondo T, Amrhein N. Blasticidin S deaminase as a positive selection marker in plants and animal cells. Plant Cell Physiol. 1999. doi:10.1093/oxfordjournals.pcp.a029571
