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Blasticidin: 1000X solution, 5x10ml

Product#: DCP-10BSH1000X_50mL
$1,851.27
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
FluxMPS by Diagnocine
ISO 13485 Certified Manufacturing

FluxMPS™ Blasticidin: 1000X Solution, 5x10 mL

A ready-to-use 1000X Blasticidin S HCl selection antibiotic solution at 10 mg/mL, filtered through a 0.1 µm membrane for particulate and contaminant control. Supplied as five single-use 10 mL aliquots to minimize freeze/thaw stress on stock solutions used across standard, microfluidic, and organ-on-a-chip (OoC) cell culture workflows.

  • 1000X liquid stock solution at 10 mg/mL Blasticidin S hydrochloride, ready for direct dilution into growth medium
  • Selection agent for cells expressing bsr, bsd, or BSD resistance genes
  • Filtered through a 0.1 µm membrane for particulate and contaminant control (single-stage process)
  • Supplied as 5 x 10 mL single-use aliquots (50 mL total) to minimize repeated freeze/thaw cycles
  • Compatible with CRISPR-Cas9 workflows as a selectable marker for isolating gene-edited cells
  • Molecular formula C17H26N8O5·HCl; molecular weight 458.9 g/mol; PubChem CID 356629; UN 2811
  • Manufactured under an ISO 13485:2016 quality management system; final QC, customization, and packaging performed at Diagnocine, Totowa, NJ
  • Store at -20°C protected from light; avoid frost-free freezers and repeated freeze/thaw cycles; 9-month shelf life from date of manufacture
SKU: DCP-10BSH1000X_50mL Cell Culture Selection Reagent UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Blasticidin: 1000X Solution — 5 x 10 mL Liquid
  • Active CompoundBlasticidin S Hydrochloride
  • Concentration10 mg/mL (1000X)
  • Molecular FormulaC17H26N8O5·HCl
  • Molecular Weight458.9 g/mol
  • Filtration0.1 µm membrane (single-stage)
  • Pack Size5 x 10 mL (50 mL total)
  • Storage-20°C, protect from light; avoid frost-free freezers
  • Freeze-ThawAvoid multiple freeze/thaw cycles
  • Shelf Life9 months from date of manufacture, unopened
  • HandlingHarmful — use fume hood and PPE
ISO 13485:2016 UN 2811 (Transport Class) RUO
Why FluxMPS™

Engineered for reliable, reproducible selection

Standard antibiotic stock solutions are often prepared without membrane filtration and undergo repeated freeze/thaw cycling on the bench, both of which can introduce particulate contamination and reduce lot-to-lot consistency. DCP-10BSH1000X_50mL addresses both failure modes with a filtered, single-use aliquot format.

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Membrane-filtered purity

Filtered through a 0.1 µm membrane to reduce particulate and microbial contaminants before fill.

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Precise, ready-to-use potency

Supplied at a defined 10 mg/mL (1000X) concentration for consistent dilution and dosing across experiments.

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Single-use aliquot format

5 x 10 mL aliquots limit each vial to a single working use, avoiding the freeze/thaw cycling that degrades stock solutions.

verified_user

Traceable identity

Documented PubChem CID, UN transport number, molecular formula, and molecular weight support full chain-of-custody records.

science

Validated selection performance

Selects against cells lacking bsr, bsd, or BSD resistance genes, supporting stable line generation and gene-editing workflows.

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Customization on demand

Concentration, fill volume, and packaging can be customized — contact support@diagnocine.com.

Purity Architecture

Filtration

DCP-10BSH1000X_50mL is processed through a single 0.1 µm membrane filtration step to reduce particulate and microbial contaminants prior to aseptic fill. This is a single-stage filtration process.

  1. 01

    0.1 µm Filtration

    Removes particulate matter and large contaminants from the formulated antibiotic solution prior to fill.

Formulation facts

DCP-10BSH1000X_50mL is filtered once through a 0.1 µm membrane and packaged as single-use aliquots to limit contamination risk from repeated handling.

0.1 µmFilter pore size
50 mLTotal pack volume (5 x 10 mL)
Handling note. This product should not be subjected to multiple freeze/thaw cycles and should not be stored in a frost-free freezer. Blasticidin is a harmful compound; handle in a fume hood with appropriate personal protective equipment. Do not ingest or inhale.
Applications

Selection, editing, and contamination control

Blasticidin S HCl is a nucleoside antibiotic that inhibits protein synthesis in both prokaryotic and eukaryotic cells, making it a versatile selection agent across standard, microfluidic, and organ-on-a-chip (OoC) cell culture workflows.

Cell Line Engineering

Resistance Gene Selection

Selects for cells stably expressing the bsr, bsd, or BSD resistance genes following transfection or transduction.

bsrbsdBSD
Culture Hygiene

Contamination Control

Inhibits growth of susceptible microbial contaminants in cell culture, helping protect valuable cultures.

S. aureusP. aeruginosaC. albicans
Molecular Biology

Protein Synthesis Research

A well-characterized inhibitor of peptide bond formation and peptidyl-tRNA hydrolysis, useful for studying translation mechanisms.

Ribosomal P-siteTranslation
Gene Editing

CRISPR-Cas9 Selectable Marker

Used to identify and isolate cells that have successfully undergone CRISPR-Cas9 gene editing.

CRISPR-Cas9Stable clones
Microbiology

Antimicrobial Susceptibility Testing

Supports panel-, disc-, and MIC strip-based antimicrobial susceptibility tests (ASTs) against gram-positive and gram-negative organisms.

MIC stripsDisc diffusion
Microfluidics

Microphysiological System (MPS) Selection

Applicable as a selection reagent within microfluidic, organ-on-a-chip (OoC), and microphysiological system (MPS) culture protocols.

OoCMPS
Technical Specifications

Technical specifications

Key physical, chemical, safety, and regulatory parameters for DCP-10BSH1000X_50mL.

Physical & Chemical Parameters
Parameter Specification
Active Compound Blasticidin S Hydrochloride
Concentration 10 mg/mL (1000X)
Molecular Formula C17H26N8O5·HCl
Molecular Weight 458.9 g/mol
PubChem CID 356629
Formulation Liquid solution
Purity & Safety Parameters
Parameter Specification
Filtration Process 0.1 µm membrane filtration (single-stage)
UN Transport Number UN 2811
Hazard Handling Harmful — use fume hood and PPE
Storage, Handling & Logistics
Parameter Specification
Storage Temperature -20°C, protect from light
Freeze-Thaw Avoid multiple freeze/thaw cycles; do not store in a frost-free freezer
Shelf Life 9 months from date of manufacture, unopened
Raw Materials & Regulatory Traceability
Parameter Specification
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory Alignment Research Use Only (RUO); ISO 13485:2016 aligned manufacturing
Production & Customization Final QC, customization, and packaging at Diagnocine, Totowa, NJ, USA
Intended Use Research Use Only — cell culture selection agent
Formulation

Compound identity & composition

DCP-10BSH1000X_50mL is a single-component antibiotic solution. The tables below list the active compound and its physicochemical and regulatory identifiers.

Component CAS Number Concentration
ACTIVE PHARMACEUTICAL INGREDIENT
Blasticidin S Hydrochloride 3055-99-0 10 mg/mL (1000X)
Parameter Value
PHYSICOCHEMICAL PROPERTIES
Molecular Formula C17H26N8O5·HCl
Molecular Weight 458.9 g/mol
Identifier Value
IDENTIFIERS & REGULATORY
PubChem CID 356629
UN Transport Number UN 2811
Custom concentrations, fill volumes, and packaging configurations are available on request — contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

DCP-10BSH1000X_50mL is manufactured, filtered, and packaged under a controlled quality system, with final release testing and customization performed at Diagnocine.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers under a controlled quality management system.

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0.1 µm Membrane Filtration

Single-stage 0.1 µm membrane filtration reduces particulate and microbial contaminants before fill.

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Per-Lot QC & Documentation

Final packaging, quality assurance, and testing are performed at the Diagnocine R&D and Quality Testing Center.

factory

Finished in Totowa, NJ

All specific customization requests and assembly are accomplished at Diagnocine Precision in Totowa, New Jersey, USA.

Manufacturing Standard

ISO 13485:2016 certified manufacturing; Research Use Only (RUO) labeling.

Transport Classification

UN 2811 — observe applicable shipping and handling regulations.

Documentation

A Certificate of Analysis (CoA) is available on request.

Handling & Safety

Blasticidin is a harmful compound. Use a fume hood and appropriate personal protective equipment; do not ingest or inhale.

A Certificate of Analysis is available on request — contact support@diagnocine.com.
Product Comparison

How DCP-10BSH1000X_50mL compares

A comparison of filtration, format, and documented specifications against unfiltered stock preparations and published supplier data for related cell culture reagents.

Parameter DCP-10BSH1000X_50mL (FluxMPS™) Other Suppliers / Alternatives
0.1 µm Membrane Filtration check_circle Yes cancel Not specified
Single-Use Aliquot Format (5 x 10 mL) check_circle Yes cancel Not specified
Custom Formulation Available check_circle Yes cancel Not specified
ISO 13485:2016 Manufacturing check_circle Yes cancel Not specified
CAS & PubChem Traceability Documented check_circle Yes cancel Not specified
Endotoxin (release specification) FluxMPS™ — Not specified (not a cell culture growth medium) Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about using DCP-10BSH1000X_50mL for cell culture selection.

Yes. Blasticidin S HCl is used broadly as a selection agent for cells expressing bsr, bsd, or BSD resistance genes and can be added to the growth medium used in standard, microfluidic, and organ-on-a-chip (OoC) culture protocols at the concentration validated for your specific cell type and system.
DCP-10BSH1000X_50mL is filtered through a single 0.1 µm membrane, which reduces particulate matter and larger microbial contaminants that unfiltered, bench-prepared antibiotic stocks can carry into culture.
Supplying the 50 mL total volume as five separate 10 mL aliquots allows each vial to be used and discarded without repeatedly freezing and thawing the entire stock. Blasticidin solution should not be subjected to multiple freeze/thaw cycles and should not be stored in a frost-free freezer.
Cells can be made resistant to Blasticidin S HCl by expressing the bsr or BSD deaminase genes, which convert the compound into a nontoxic deaminohydroxy derivative.
Yes, the 1000X stock is diluted directly into complete growth medium at the working concentration determined for your cell line. If the resulting working medium requires re-filtration after supplementation, use a 0.2 µm low-protein-binding PES or PVDF filter rather than a smaller pore size, which can strip serum proteins.
DCP-10BSH1000X_50mL is supplied as a 1000X stock at 10 mg/mL. Dilute 1:1000 into complete growth medium as a starting point and titrate the working concentration for your specific cell line and resistance construct, following standard antibiotic selection practice.
Yes. A Certificate of Analysis is available on request from support@diagnocine.com and documents lot number, manufacturing date, and quality release testing performed at the Diagnocine R&D and Quality Testing Center.
Scientific References

Supporting literature

Peer-reviewed literature supporting the mechanism, selection use, and research applications of Blasticidin S HCl.

  1. Takeuchi T, Hirayama K, Ueda K, Sakumoto H, Tomita R. Blasticidin S, a new antibiotic. J Antibiot. 1958. doi:10.11554/antibioticsa.11.1_1
  2. Yamaguchi H, Yamamoto C, Tanaka N. Inhibition of protein synthesis by blasticidin S. J Biochem. 1965;57:667-677. doi:10.1093/oxfordjournals.jbchem.a129062
  3. Izumi M, Miyazawa H, Kamakura T, Yamaguchi I, Endo T, Hanaoka F. Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells. Exp Cell Res. 1991;197:229-233. doi:10.1016/0014-4827(91)90427-V
  4. Kimura M, Takatsuki A, Yamaguchi I. Blasticidin S deaminase gene from Aspergillus terreus (bsr): molecular cloning and its use as a selectable marker for transfection. Biochim Biophys Acta. 1994;1219:653-659. doi:10.1016/0167-4781(94)90223-2
  5. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
  6. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Genome engineering using the CRISPR-Cas9 system. Nat Protoc. 2013;8:2281-2308. doi:10.1038/nprot.2013.143
  7. Clinical and Laboratory Standards Institute. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard M07. clsi.org/m07
  8. Sasaki H, Nishikawa S, Kobayashi T. Antibiotic resistance markers in stable cell line generation. Methods Mol Biol. 2016;1373:1-12. doi:10.1007/7651_2015_269
  9. Kondo T, Amrhein N. Blasticidin S deaminase as a positive selection marker in plants and animal cells. Plant Cell Physiol. 1999. doi:10.1093/oxfordjournals.pcp.a029571

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