BioMasher Series Comparison

*See below for detailed information. You can not order on this page.

 

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The Best Way for Homogenizing Your Samples
Disposable Homogenizer the "BioMasher"

 

BioMasher Series Comparison

Small to Large Size Tissues, Plants, Cell Pellets, Frozen Samples

• FAST TISSUE GRINDING to HOMOGENIZE
• DISPOSABLE & Premium Certified Plastics
• AUTOCLAVABLE & STERILIZED
• Multiple Types for various experimental designs

 

Product Lineup

Product Name		BioMasher
		☞ I			☞ II		☞ III	☞ V		☞ SP
Procedure
Shape
Tube Size		1.5 ml					2.0 ml	15 ml
Collection Amount		Small			Large		Large	Large
Heat		Heatless			Controllable		Controllable	Controllable		Controllable
Device		Centrifuge			Unnecessary		Centrifuge	Centrifuge		Unnecessary
Samples' types*	Soft	Excellent			Excellent		Excellent	Excellent		Excellent
	Hard	Not good			Excellent		Excellent	Excellent		Excellent
	Cultured Cells	Not appropriate			Not appropriate		Not appropriate	Not appropriate		Not appropriate
Go Youtube Demo-View
Sterile / Non-sterile		Non-sterile			Sterile	Non-sterile	Non-sterile	Sterile	Non-sterile	Sterile	Non-sterile
Filter types   // O-ring ★ (Nitrile rubber)		PE★	PP★		PP★		PP★	PP★		PP★
		O-ring	O-ring	No ring
Qty.		30 / 120	30 / 120	30 / 120	100	100	50	20 / 100	20 / 100	40 / 100	40 / 100
Order

 

O-ring ^★:  O-ring is suitable for homogenizing soft tissue with centrifugal force alone. In particular, it is suitable for simultaneous processing of many samples and for infectious samples.

Without an O-ring(No ring): 
Hard tissue may not be homogenized sufficiently by centrifugal force alone. The model without an O-ring is suitable when grinding the sample on the filter area before centrifugation to enhance disruption efficiency.

PE^★,  PP^★: polyethylene (PE) or polypropylene (PP).  The PE filter has smaller pore size than the PP filter resulting in finer disruption with the PE filter. 

    

Appropriate Samples Type* for BioMasher series

Lab#	BioMasher I	BioMasher II	BioMasher III	BioMasher V	BioMasher SP
Soft Tissue	Excellent	Excellent	Excellent	Excellent	Excellent
Hard Tissue	Not good	Excellent	Excellent	Excellent	Excellent
Yeast	Not appropriate	Not appropriate	Not appropriate	Not appropriate	Not appropriate
Culture Cell	Not good	Not appropriate	Not good	Not good	Not appropriate

Product	Features	Procedure
BioMasher - I	* Two types of pestle (with O-ring or without O-ring) are available.  To avoid spill of liquid infectious samples, it is good for using with "O-ring" pestle. * For soft tissue samples: Put samples into a filter cup and insert pestle. Then centrifuge at 10,000 to15,000 x g for 10 to 30 sec. * For hard tissue samples: The tip of the pestle has " + " shape blade. This makes it possible to grind samples (which are difficult to be homogenized by only centrifuge) by pushing and twisting the pestle.
BioMasher - II	* The combination of texture both pestle and inside of tube, homogenization efficiency is highly increased. * Centrifuge is not required. * Put samples into tube and insert pestle. Grinding samples by pestle by hand or motorized rotator named "Power Masher II". *Please see the handling video here
BioMasher - III	*Technology of BioMasher- I and II are combined. * Put samples into filter tube and insert pestle. Grinding samples by pestle by hand or motorized rotator named "Power Masher II". After that, centrifuge at 6,000 to 10,000 x g for 10 to 30 sec. *Please see the handling video from here.
BioMasher - V
BioMasher - SP
Power Masher II

  

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Disposable homogenizer

 

BioMasher I

BioMasher I  order pages

1.5 ml tube

2.0 ml tube

 

BioMasher II

BioMasher II  order pages

BioMasher III

BioMasher III  order pages

 

BioMasher V

BioMasher V  order pages

 

Why Powermasher II (Pestle motor for BioMasher II, III, V, & SP)

Powermasher II  order page

 

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Comparison of RNA extraction efficiency among BioMasher I, II, and III

[Method]
RNA was extracted from various organs of a mouse (Crlj:CD1 [ICR]: Adult mouse of 8 weeks or older) using BioMasher I-III according to the attached protocol. As a control, RNA was extracted by using a pestle of SSI and a 1.5 mL tube of TreffLab.

[Results]
The results are summarized below.

Tissue	RNA Extraction Ratio (RNA (μg)/Sample (mg))
	BioMasher I	BioMasher II	BioMasher III	Pestle/SSI &
				1.5 mL tube/TreffLab
Liver	1	1.5	1.32	0.92
Kidney	0.26	1.87	4.66	0.15
Cerebrum	0.16	1.14	-	0.12
Spleen	0.15	5.53	-	0.36
Heart	0.08	1.02	0.5	0.12
Tail	0.03	0.43	-	0.16
Muscle	0.02	0.8	0.24	0.43

*Choose appropriate model of BioMasher for the maximum extraction ratio of RNA depending on tissue type.


References

• Tsuji M. et al., An immunostimulatory glycolipid that blocks SARS-CoV-2, RSV, and influenza infections in vivo. Nat Commun. 2023 Jul 5;14(1):3959. 
• Ittiprasert W. et al., Targeted insertion and reporter transgene activity at a gene safe harbor of the human blood fluke, Schistosoma mansoni. Cell Rep Methods. 2023 Jul 24;3(7):100535.