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FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-BMEH-B1X
$44.00
DCP-BMEH-B1X
Availability:
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Without Sodium Bicarbonate Without Sodium Pyruvate

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) cell culture medium buffered with 25 mM HEPES and formulated without sodium bicarbonate, engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Quadruple-stage filtration train: 0.1 µm (Prefiltration I) → 0.04 µm (Final filtration I) → 0.1 µm (Prefiltration II) → 0.04 µm (Final filtration II — Polish)
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> BET)
  • 25 mM HEPES buffering with no added sodium bicarbonate — pH stability in ambient air, reduced CO2 dependency
  • Contains 1.0 g/L D-glucose and 292 mg/L L-glutamine; sodium pyruvate not added
  • Manufactured under an ISO 13485:2016 quality management system; final fill & finish at Diagnocine, Totowa, NJ
  • Prepared with Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon (TOC) control
  • Available in 500 mL and 1000 mL pack sizes
  • Custom pH, glucose, salts, HEPES, and nutrient composition available on request — contact support@diagnocine.com
DCP-BMEH-B1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Available sizes: 500 mL, 1000 mL
Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L)
  • L-Glutamine292 mg/L
  • Sodium PyruvateNot added
  • HEPES5958 mg/L (25 mM)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)280 - 320 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ addresses these failure modes with a validated four-stage filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filter stage retains particles down to sub-mycoplasma size (0.2–0.3 µm); USP <788> Method 1 (light obscuration) particulate compliance verified per lot.

target

Total metabolic control

This formulation supplies 1.0 g/L D-glucose and 292 mg/L L-glutamine as defined carbon and nitrogen sources. Glucose-free custom formulations are available on request for Warburg-effect and metabolic flux studies.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm), controlled for trace metals and organic carbon (TOC) to minimize background contribution from the aqueous base.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy and live-cell biosensor workflows. Note: this formulation contains phenol red, which absorbs in the visible range; a phenol red-free variant is available on request for fluorescence-sensitive assays.

science

Rich, stable nutrient profile

Micro-batch manufacturing supports consistent lot-to-lot amino acid and vitamin concentrations for reproducible long-term perfusion studies.

tune

Customization on demand

pH, glucose concentration, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid is a ready-to-use formulation processed through a four-stage serial filtration sequence that reaches 0.04 µm — addressing mycoplasma-sized organisms (0.2–0.3 µm) and subvisible particulates that 0.22 µm filtration cannot retain.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final-filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass through a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge and providing redundancy across the train.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish performed in an ISO Class 5 (Class 100) laminar-flow workstation.

Performance vs. conventional media

By reaching a 0.04 µm final pore size across four sequential stages (two prefilter/final-filter pairs), FluxMPS™ delivers approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration.

5×
 
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration (not tested per lot per USP <63>).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid (DCP-BMEH-B1X) Quadruple-stage filtration system diagram showing four sequential stages: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, and 0.04 micron Final filtration II Polish for organ-on-a-chip and microfluidic cell culture applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering a microfluidics-suitable purity baseline for OoC and MPS applications.
© Diagnocine® — DCP-BMEH-B1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid is validated for use across organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: reduces risk of particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: supports flow rate consistency across multi-week automated runs

Inquiry Required: The 0.01 µm MPS Grade variant is available by special order. Contact support@diagnocine.com to request this variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation helps prevent microchannel clogging and supports laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined carbon source and controlled endotoxin background support metabolic flux analysis and Warburg effect studies.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-filtered formulation supports sensitive iPSC differentiation protocols where endotoxin and particulates cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurements in perfusion models.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined glucose and glutamine concentrations support isotope tracing and metabolic flux workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate load supports high-content confocal imaging and optical biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid undergoes the complete quality-release battery listed below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation L-Glutamine + Phenol Red + HEPES + Calcium + Magnesium + Glucose; without Sodium Bicarbonate, without Sodium Pyruvate
Appearance Red-colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> 280 - 320 mOsm/kg H2O
Glucose 1000 mg/L (1.0 g/L)
L-Glutamine 292 mg/L
Sodium Pyruvate Not added
Phenol Red Present (11.000 mg/L)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016 ISO 13485
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement HEPES-buffered — reduced CO2 dependency
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Available pack sizes 500 mL, 1000 mL
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers. All ingredient names and mg/L values are reproduced from the manufacturer specification. Total: 30 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others). Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
L-Arginine hydrochloride 1119-34-2 21.100
L-Cystine dihydrochloride 30189-89-0 15.650
L-Glutamine 56-85-9 292.000
L-Histidine hydrochloride 1007-42-7 10.500
L-Isoleucine 73-32-5 26.200
L-Leucine 61-90-5 26.200
L-Lysine hydrochloride 657-27-2 36.480
L-Methionine 63-68-3 7.500
L-Phenylalanine 63-91-2 16.500
L-Threonine 72-19-5 23.800
L-Tryptophan 73-22-3 4.000
L-Tyrosine disodium salt 69847-45-6 25.950
L-Valine 72-18-4 23.400
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 1.000
D-Biotin 58-85-5 1.000
D-Ca-Pantothenate 137-08-6 1.000
Folic acid 59-30-3 1.000
Nicotinamide 98-92-0 1.000
Pyridoxal hydrochloride 65-22-5 1.000
Riboflavin 83-88-5 0.100
Thiamine hydrochloride 67-03-8 1.000
i-Inositol 87-89-8 2.000
OTHERS
D-Glucose 50-99-7 1000.000
HEPES 7365-45-9 5958.000
Phenol red sodium salt 34487-61-1 11.000
Customization: pH, glucose concentration, salt balance, HEPES concentration, and full nutrient profile are available on request. Contact support@diagnocine.com with your specifications.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release processes for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity Type 1 water, controlled for trace metals and organic carbon (TOC).

biotech

ISO Class 5 Fill & Finish

Aseptic filling performed in validated laminar-flow (ISO Class 5 / Class 100) workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports consistent lot-to-lot nutrient concentrations for reproducible perfusion studies and long-term OoC experiments.

Endotoxin — USP <85> BET

LAL assay performed per batch. Release specification: < 0.05 EU/mL. See the batch-level quality control note below.

Particulate — USP <788> Method 1

Light obscuration particle count test confirms ≥10 µm and ≥25 µm particulate compliance on every lot.

Osmolality — USP <785>

Freezing-point osmometry performed per USP <785>. Result: 280 - 320 mOsm/kg H2O.

Documentation — CoA & Full Lot Records

Certificate of Analysis available for every lot, including full QC panel, raw material traceability, and release signatures.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-BMEH-B1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter DCP-BMEH-B1X (FluxMPS™) Conventional BME (0.22 µm) Standard BME alternative
Grade Microfluidics Suitable (0.04 µm final) Not specified Not specified
Buffering system HEPES-buffered (25 mM), bicarbonate-free Sodium bicarbonate-buffered Sodium bicarbonate-buffered
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES w/o Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. This formulation is processed through FluxMPS™'s quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), producing an ultra-low particulate baseline suited to microfluidic channel geometries in OoC and MPS devices. It is Microfluidics Suitable at a 0.04 µm final cut-off.
Standard 0.22 µm filtration does not retain mycoplasma-sized organisms (0.2–0.3 µm diameter) or many subvisible particulates. This formulation is filtered through two prefilter/final-filter pairs reaching 0.04 µm, giving an additional retention step beyond conventional single-pass filtration.
This formulation uses 25 mM HEPES as its buffering system instead of sodium bicarbonate, maintaining pH stability in ambient air — useful for open-well imaging, portable incubation, and microfluidic perfusion outside a CO2 incubator. If your protocol requires bicarbonate buffering, contact support@diagnocine.com for a custom formulation.
No. This HEPES-buffered, bicarbonate-free formulation is designed for reduced CO2 dependency. Standard CO2 incubation remains compatible if required by your protocol.
Yes. This medium can be supplemented with FBS (typically 5–20%), growth factors, antibiotics, or other additives per standard cell culture practice. When adding serum or other protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane — never a smaller pore size, which can strip serum of functional components. Add supplements immediately before use.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> Bacterial Endotoxins Test (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. A Certificate of Analysis is available on request from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), and lot traceability information. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting the scientific rationale for ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
  6. Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
  7. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  8. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  9. Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
  10. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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