FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES: 1X Liquid

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES: 1X Liquid

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC) and microphysiological systems (MPS). A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

• Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final polish for microchannel-safe purity
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET)
• Basal Medium Eagle (BME) base supplemented with 25 mM HEPES and 26 mM sodium bicarbonate (2200 mg/L) for buffering redundancy; pH 7.4 (USP <791>)
• 1.0 g/L D-glucose and 292 mg/L L-glutamine; phenol red sodium salt included as a visual pH indicator
• Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) under an ISO 13485:2016 quality management system
• ISO Class 5 aseptic fill & finish performed at Diagnocine’s Totowa, NJ facility
• Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and particulate loads that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ addresses these failure modes at the source.

Quadruple-stage filtration system

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES: 1X Liquid is a ready-to-use formulation processed through a four-stage serial filtration sequence that reaches 0.04 µm — addressing mycoplasma-sized particulates, subvisible debris, and bioburden that 0.22 µm filtration alone cannot address.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm final-filter cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, including mycoplasma-sized (0.2–0.3 µm) organisms.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge from residual particulate load.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill & finish performed in a validated laminar-flow (ISO Class 5 / Class 100) workstation.

Designed for next-generation cell culture platforms

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES: 1X Liquid is validated for use across organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this same formulation is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion; it adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish used in the Microfluidics Suitable line described on this page.

• Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
• Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
• Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: The 0.01 µm MPS Grade variant is available by special order. Contact support@diagnocine.com to request this variant.

Batch-release quality parameters

Every production batch of FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES: 1X Liquid undergoes the complete quality-release battery listed below before shipment.

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from the manufacturer specification (31 components across 4 categories). Custom compositions available on request.

ISO 13485:2016 manufacturing & compliance

FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES: 1X Liquid is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine Precision in Totowa, New Jersey, USA.

How DCP-BMEH1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Basal Medium Eagle (BME), 25mM HEPES: 1X Liquid and Microfluidics Suitable cell culture media.

Supporting literature

Peer-reviewed publications supporting the scientific rationale for ultra-filtered media in microfluidic and organ-on-a-chip cell culture applications.

1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
3. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
5. Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
6. Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
7. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
8. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
9. Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
10. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175