FluxMPS™ Basal Medium Eagle (BME): 1X Liquid
FluxMPS™ Basal Medium Eagle (BME), 1X Liquid
FluxMPS™ Basal Medium Eagle (BME), 1X Liquid is an MPS-grade, ultra-filtered cell culture medium manufactured through a proprietary Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), delivering approximately 5× cleaner media than conventional 0.22 µm-filtered formulations by particulate count. Engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological system (MPS) platforms where sub-micron particulates cause sensor drift, microchannel occlusion, and compromised cell viability.
- Quadruple-stage nanofiltration: 0.1 µm pre-filter ×2 + 0.04 µm sterile filter ×2 — the industry’s most rigorous filtration stack for ready-to-use basal media
- Ultra-low endotoxin: < 0.05 EU/mL (USP <85> BET-verified), eliminating TLR4-mediated inflammatory artifacts in sensitive primary cell models
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm), reducing metal ion and organic impurity backgrounds
- ISO Class 5 (Class 100) aseptic fill & finish under ISO 13485:2016 QMS — traceable lot release
- Phenol Red indicator formulation
- Microchannel-safe: USP <788> Method 2 particulate specification — ≤300 particles ≥10 µm/mL, ≤90 particles ≥25 µm/mL
- Custom formulations available — pH, glucose, HEPES, salts, and nutrient modifications on request
- D-Glucose1,000 mg/L
- L-Glutamine292 mg/L
- Sodium PyruvateNot included
- Phenol Red11 mg/L
- pH (USP <791>)7.4
- Osmolality (USP <785>)230.00 - 270.00 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm×2 + 0.04µm×2
- Storage2 – 8°C, protected from light
- Shelf Life12 months
Engineered where standard media fails
Conventional 0.22 µm-filtered Basal Medium Eagle passes mycoplasma-sized particles (200–300 nm), sub-visible aggregates, and endotoxin fragments that clog microfluidic channels, trigger innate immune responses, and corrupt downstream biosensor or TEER signals. FluxMPS™ BME closes these failure modes with a four-stage nanofiltration cascade and ISO Class 5 manufacturing — delivering the only ready-to-use BME formulation validated for MPS and organ-on-a-chip platforms.[1,2]
Microchannel-Safe Purity
0.04 µm final filtration removes particles that 0.22 µm membranes miss. USP <788> Method 2 sub-visible particulate compliance ensures every batch is safe for <100 µm channel geometries.
Total Metabolic Control
L-Glutamine-containing base formulation provides a defined carbon and nitrogen source to support energy metabolism and biosynthesis. Supports Warburg-effect studies, metabolic flux analysis, and amino acid drop-in experiments without baseline interference.[3]
Ultrapure-Grade Water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm, USP <85>), minimizing heavy metal ions and trace organic contaminants that interfere with sensitive metabolomics and electrophysiology readouts.
Low Background for Imaging
Ultra-clean formulation suppresses autofluorescence and non-specific optical backgrounds, making FluxMPS™ BME the preferred medium for confocal live-cell imaging, TEER biosensors, and optical microfluidic platforms.[4]
Rich, Stable Nutrient Profile
Complete inorganic salt matrix (NaCl 6,800 mg/L, NaHCO₃ 2,200 mg/L), 12 essential amino acids, and 8 vitamins — micro-batch manufactured for lot-to-lot consistency critical in perfusion-based MPS experiments.
Customization on Demand
pH, glucose concentration, HEPES buffering, salt balance, and nutrient composition are available as custom modifications. Contact support@diagnocine.com for bespoke formulations.
Quadruple-stage filtration system
FluxMPS™ BME is the only ready-to-use Basal Medium Eagle manufactured through a four-stage nanofiltration cascade terminating at 0.04 µm — a pore size 5.5× smaller than industry-standard 0.22 µm membranes. Each stage serves a distinct purification function, delivering cumulative removal of particulates, bacteria, and mycoplasma-scale contaminants that accumulate in conventional media preparations.
-
1
0.1 µm Pre-filtration I — Large Particulate Removal
First-pass removal of large aggregates, cell debris, and macro-particulates. Extends downstream membrane life and reduces bioburden load before nanofiltration stages.
-
2
0.04 µm Pre-filtration II — Sub-Mycoplasma Polishing
Fine particulate, bacterial, and mycoplasma-sized contaminant retention. The 40 nm pore size provides a sterilizing barrier far below the 200–300 nm size range of mycoplasma organisms.
-
3
0.1 µm Sterile-filtration I — Redundancy Pass
Second-pass 0.1 µm sterile filtration provides process redundancy, ensuring no breakthrough from the first stage propagates forward. Mandatory for ISO Class 5 certified fill lines.
-
4
0.04 µm Sterile-filtration II — Final Polish & Aseptic Fill
Ultimate 40 nm polish immediately prior to ISO Class 5 aseptic fill. Guarantees the lowest achievable sub-visible particulate count and provides the sterilizing assurance required for MPS-grade release.
Performance vs. conventional media
Independent particulate counting (USP <788> Method 2) consistently demonstrates that FluxMPS™ BME contains approximately 5× fewer sub-visible particles per mL compared to leading 0.22 µm-filtered basal media formulations. In microfluidic chip experiments with ≤100 µm channels, this reduction directly correlates with extended channel patency, lower instrument-downtime rates, and more reproducible perfusion profiles.[5]
0.22 µm filtered media
sub-mycoplasma barrier
© Diagnocine® - DCP-BME1X
Optimized for next-generation cell platforms
FluxMPS™ BME’s ultra-clean formulation, precise ionic balance, and microchannel-safe particulate profile make it the ideal basal medium for high-demand research platforms — from organ-on-a-chip perfusion systems to high-resolution metabolomics and live-cell optical sensing.[1,4,6]
Automated Bioreactors & Robotics
For automated perfusion bioreactors, robotic liquid-handling platforms, and high-throughput microfluidic screening systems, an optional 0.01 µm (10 nm) ultra-filtered variant of FluxMPS™ BME is available. This advanced grade provides near-absolute exclusion of nanoparticulates, protecting precision valves, flow sensors, and sub-micron microchannels from fouling during extended automated runs.
- Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulates that transit standard 0.04 µm membranes, eliminating cumulative fouling in long-duration perfusion experiments
- Valve & Sensor Protection: Ultra-clean media extends the operational lifetime of microfluidic valves, pressure transducers, and optical flow sensors in automated bioreactor systems
- Extended Perfusion Stability: Reduced particulate and endotoxin load sustains baseline-stable TEER, impedance, and biosensor readings across multi-week perfusion protocols
Inquiry Required: The 0.01 µm (10 nm) grade is produced on a project basis. To request this specification, contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
FluxMPS™ BME’s ultra-low particulate count and sub-mycoplasma filtration make it the preferred basal medium for organ-on-a-chip, tissue-on-a-chip, and body-on-a-chip platforms requiring sustained channel patency.[1]
Warburg Effect & Metabolic Research
Defined carbon source and low-endotoxin background enables precise metabolic flux analysis and Warburg effect studies.
iPSC-Derived Models
Ultra-clean, low-endotoxin BME base supports defined iPSC differentiation protocols where conventional media impurities corrupt epigenetic and transcriptional outcomes in neuronal, cardiomyocyte, and hepatocyte models.[7]
Endothelial & Primary Cells
Physiological osmolality and defined salt matrix (NaCl 6,800 mg/L, KCl 400 mg/L) sustains endothelial monolayer integrity in perfused vascular-chip and tube-formation assays with primary cell cultures.[2]
Metabolic Flux Analysis
Defined, stable nutrient composition provides the clean metabolic background required for ¹³C isotope tracing, Seahorse XF extracellular flux analysis, and NMR-based metabolomics without confounding media-derived signals.[3,8]
Microscopy & Optical Sensing
Ultra-low particulate and minimal autofluorescence background make FluxMPS™ BME ideal for confocal live-cell imaging, fluorescence-based biosensors, and TEER transepithelial resistance monitoring in transparent microfluidic chips.[4]
Analytical & quality release data
Each lot of FluxMPS™ BME is released against a comprehensive Certificate of Analysis covering physical, chemical, sterility, and particulate parameters. All specifications are measured by validated USP and ISO methods, with full traceability to raw material lots.
| Parameter | Specification |
|---|---|
| Formulation | 1X Liquid; L-Glutamine-free; With Phenol Red |
| Appearance | Clear, red-orange liquid; no visible particulates |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 270 – 310 mOsm/kg |
| D-Glucose | 1,000 mg/L (5.56 mM) |
| L-Glutamine | 292 mg/L |
| Sodium Pyruvate | Not included |
| Phenol Red | 10 mg/L (included) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | Sterile — 14-day test, no growth |
| Mycoplasma USP <63> equiv. | Negative |
| Particulate ≥10 µm USP <788> M2 | ≤ 300 particles/mL |
| Particulate ≥25 µm USP <788> M2 | ≤ 90 particles/mL |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. ISO 13485 | ISO 13485:2016 QMS |
| Fill environment | ISO Class 5 (Class 100) laminar flow |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from direct light |
| Freeze-thaw | Do not freeze — precipitation of salts may occur |
| Shelf life | 12 months from date of manufacture |
| Shipping condition | Cold pack |
| CO₂ requirement | Yes — 5% CO₂ atmosphere required (NaHCO₃ buffered) |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell culture / reagent grade |
| Traceability | Full lot-to-raw material traceability under ISO 13485:2016 |
| Manufacturing QMS ISO 13485 | ISO 13485:2016, 21 CFR Part 820 aligned |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch precision fill & finish |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
Complete formulation of FluxMPS™ Basal Medium Eagle (BME), 1X Liquid. All values in mg/L; CAS numbers provided for reference. Every ingredient is released per-lot against this specification. Custom adjustments to any component — including L-Glutamine, HEPES, glucose, or salt concentrations — are available on request.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium Chloride (CaCl₂) (dihydrate) | 10035-04-8 | 265.000 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.720 |
| Potassium Chloride (KCl) | 7447-40-7 | 400.000 |
| Sodium Bicarbonate (NaHCO₃) | 144-55-8 | 2200.000 |
| Sodium Chloride (NaCl) | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Arginine HCl | 1119-34-2 | 21.100 |
| L-Cystine 2HCl | 30925-07-6 | 15.650 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride | 1007-42-7 | 10.500 |
| L-Isoleucine | 73-32-5 | 26.200 |
| L-Leucine | 61-90-5 | 26.200 |
| L-Lysine hydrochloride | 657-27-2 | 36.480 |
| L-Methionine | 63-68-3 | 7.500 |
| L-Phenylalanine | 63-91-2 | 16.500 |
| L-Threonine | 72-19-5 | 23.800 |
| L-Tryptophan | 73-22-3 | 4.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 25.950 |
| L-Valine | 72-18-4 | 23.400 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline Chloride | 67-48-1 | 1.000 |
| D-Biotin | 58-85-5 | 1.000 |
| D-Calcium Pantothenate | 137-08-6 | 1.000 |
| Folic Acid | 59-30-3 | 1.000 |
| Inositol | 87-89-8 | 2.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal HCl | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine HCl | 67-03-8 | 1.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
Built under the most rigorous standards in life science
FluxMPS™ BME is manufactured in Totowa, NJ, USA under an ISO 13485:2016 Quality Management System with 21 CFR Part 820 (cGMP) alignment. Every production step — from raw material receipt through final aseptic fill — is documented, traceable, and independently verified.
ISO 13485:2016 Quality Management System
Full ISO 13485:2016 QMS certification governs design controls, supplier qualification, in-process testing, release criteria, and complaint handling. 21 CFR Part 820 (cGMP) aligned for pharmaceutical-adjacent research environments.
Ultrapure Type 1 Water (18.2 MΩ·cm)
All formulations prepared with freshly produced Ultrapure Type 1 water meeting USP <85> specifications — 18.2 MΩ·cm resistivity, < 5 ppb total organic carbon, endotoxin-free system-validated.
ISO Class 5 Fill & Finish
Aseptic filling performed under ISO Class 5 (Class 100) unidirectional laminar airflow. Container integrity verified by visual inspection and leak testing on 100% of filled units prior to final release.
Micro-Batch Precision Manufacturing
Small-batch production ensures tight lot-to-lot consistency. In-process QC checks at each manufacturing stage are logged and retained. Full batch records available for qualified customers upon request.
Endotoxin — USP <85> BET
Limulus Amebocyte Lysate (LAL) Bacterial Endotoxin Test performed on every lot. Release specification: < 0.05 EU/mL. Validated in-house method, traceable to USP Reference Standard.
Particulate — USP <788> Method 2
Light obscuration sub-visible particulate counting on every production lot. Release limits: ≤300 particles ≥10 µm/mL and ≤90 particles ≥25 µm/mL. Approximately 5× cleaner than 0.22 µm filtered media.
Osmolality — USP <785>
Osmolality measured by freezing-point depression on every lot. Specification: 270–310 mOsm/kg, ensuring physiological compatibility for mammalian cell culture across all supported cell types.
Certificate of Analysis (CoA)
Full CoA including lot number, manufacture date, expiry, pH, osmolality, endotoxin, sterility, particulate, and composition verification available for every released lot. Request via support@diagnocine.com.
How FluxMPS BME compares
FluxMPS™ BME is benchmarked below against conventional 0.22 µm single-pass filtered BME formulations from standard suppliers. The differences are most consequential in microfluidic, MPS, and high-sensitivity assay contexts.
| Parameter | FluxMPS™ BME | Conventional BME (0.22 µm filtered) | Standard Basal Medium (single-pass 0.22 µm) |
|---|---|---|---|
| Formulation base | Eagle’s Basal Medium, standard | Eagle’s Basal Medium, standard | Eagle’s Basal Medium or MEM, standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm (220 nm) | 0.22 µm (220 nm) |
| Number of filtration stages | 4 stages | 1 stage | 1 stage |
| Mycoplasma barrier filtration | check_circle 0.04 µm barrier | cancel 0.22 µm passes mycoplasma | cancel Not validated |
| Endotoxin specification | < 0.05 EU/mL (lot-tested) | < 1.0 EU/mL typical | < 1.0 EU/mL typical |
| USP particulate compliance | check_circle USP <788> Method 2 | cancel Not specified | cancel Not specified |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Purified water, variable | Purified water, variable |
| Manufacturing QMS | check_circle ISO 13485:2016 | cancel Not certified | cancel Not certified |
| Microfluidic channel compatibility | check_circle Validated ≤100 µm | cancel Not validated | cancel Not validated |
| Custom formulation | check_circle Available on request | cancel Fixed formulation | cancel Fixed formulation |
Frequently asked questions
Common questions about FluxMPS™ Basal Medium Eagle (BME), 1X Liquid — filtration, formulation, supplementation, and quality assurance.
Supporting literature
Peer-reviewed publications supporting the use of ultra-filtered, MPS-grade basal media in organ-on-a-chip, microfluidic perfusion, metabolomics, vascular biology, and live-cell imaging applications.
- Huh D, Hamilton GA, Ingber DE. From 3D cell culture to organs-on-chips. Trends Cell Biol. 2011;21(12):745–754.doi:10.1016/j.tcb.2011.09.005
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772.doi:10.1038/nbt.2989
- Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation. Science. 2009;324(5930):1029–1033.doi:10.1126/science.1160809
- Pampaloni F, Reynaud EG, Stelzer EH. The third dimension bridges the gap between cell culture and live tissue. Nat Rev Mol Cell Biol. 2007;8(10):839–845.doi:10.1038/nrm2236
- van der Meer AD, van den Berg A. Organs-on-chips: breaking the in vitro impasse. Integr Biol. 2012;4(5):461–470.doi:10.1039/c2ib00176d
- Beebe DJ, Ingber DE, den Toonder J. Organs on Chips 2013. Lab Chip. 2013;13(18):3447–3448.doi:10.1039/c3lc90080k
- Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006;126(4):663–676.doi:10.1016/j.cell.2006.07.024
- Metallo CM, Vander Heiden MG. Understanding metabolic regulation and its influence on cell physiology. Mol Cell. 2013;49(3):388–398.doi:10.1016/j.molcel.2013.01.018
- Ronaldson-Bouchard K, Vunjak-Novakovic G. Organs-on-a-chip: a fast track for engineered human tissues in drug development. Cell Stem Cell. 2018;22(3):310–324.doi:10.1016/j.stem.2018.02.011
- Zhang YS, Aleman J, Shin SR, et al. Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. Proc Natl Acad Sci USA. 2017;114(12):E2293–E2302.doi:10.1073/pnas.1612906114
