Apoptosis

Storage and transportation

Ice bag (wet ice) transportation;

This kit is stored at -20°C and has a validity period of 12 months.

Cat.No:	Description	Size	Volume	Link
SB-G1501	Fluorescein (FITC)	100T	100 rxn
		50T	50 rxn
SB-G1502	TMR Red	100T	100 rxn
		50T	50 rxn
SB-G1504	CF488	100T	100 rxn
		50T	50 rxn
SB-G1505	CF640	100T	100 rxn
		50T	50 rxn
SB-G1507-100T	DAB (SA-HRP)	100T	100 rxn

Product description:

The breakage of chromosomal DNA in apoptosis is a gradual process. Chromosomal DNA is first degraded into large fragments of 50-300 kb under the action of endogenous nucleases, and then about 30% of chromosomal DNA is under the action of Ca²⁺ and Mg²⁺-dependent endonucleases. They are randomly cut to form 180-200 bp nucleosomal DNA polymer. Therefore, in the late stage of apoptosis, DNA will be degraded into 180-200 bp fragments, and a large number of 3'-OH ends will be exposed on the broken genomic DNA. Terminal Deoxynucleotidyl Transferase (TdT) is a template-independent DNA polymerase that can catalyze the binding of deoxynucleotides to the 3'-OH ends of broken DNA molecules. Therefore, TUNEL (TdT mediated dUTP Nick End Labeling) cell apoptosis detection kit can be used to detect the nuclear DNA breakage of tissue cells in the late stage of apoptosis. The principle is that under the action of TdT enzyme, biotin-labeled dUTP (Biotin-dUTP) is incorporated into the 3´-OH end exposed when genomic DNA is broken, and then horseradish peroxidase (Horse-radish peroxidase, HRP) labeled Streptavidin (Streptavidin-HRP, SA-HRP), detect the end of DNA labeled with Biotin, and finally perform color reaction by adding HRP substrate mixture (DAB) , So that the nucleus of apoptotic cells is stained brown, which can be detected by ordinary optical microscope. This kit has a wide range of applications and is suitable for the detection of cell apoptosis in paraffin tissue sections, frozen tissue sections, cell slides, cell smears, etc.