Anti-RagC/RRAGC Antibody, rabbit polyclonal
Product#: FNK-71-023
$346.79
Availability:
Ships in 1-2 Weeks
Anti-RagC/RRAGC Antibody, rabbit polyclonal
General information
| Cat. No. | ：FNK-71-023 |
| Size | ：100 ug |
| Host Species | ：Rabbit |
| Cross Reactivity | ：Hamster/Human/Mouse |
| Reactivity | ：Reacts with human, mouse and hamster. Not tested in other species. |
| Immunogen | ：Purified full-length human Rag C protein fused with GST |
| Application | ：Western blotting ( 1,000~ 2,000 folds dilution), Immunofluorescence staining (1/100~1/500), Not tested for other applications |
| Storage | ：Shipped at 4C or -20C. Upon arrival spin-down, aliquot and store at -20℃. |
| Form | ：Purified IgG fraction. 1 mg/ml in PBS with 50% glycerol. Filter-sterilyzed. Azide- and carrier-free. |
| Data Link | ：UniProtKB/Swiss-Prot: Q9HB90 |
Key Words GTP-Binding Protein, mTORC1, TOR signaling cascade
Function
Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids
Molecular mass
44,224 with 399 amino acids. Rag C forms a functional complex with Rag A and Rag B.
Cellular localization
Predominantly cytoplasmic. May shuttle between the cytoplasm and nucleus, depending on the bound nucleotide state of associated RagA. Post-translational modification: Acetylation and phosphorylation
Fig.1 Western blot analysis of Rag C protein in the cell lysates. Anti-RagA antiserum was used at 1/1,000 dilution. Second antibody, anti-rabbit IgG conjugated with HRP, was used at 1/10,000 dilution.
Fig.2 Immunofluorescence stainig of Rag C protein in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.5% TritonX 100 and reacted with anti-RagA antibody at 1/100 dilution As the 2nd antibody, anti-rabbit IgG antibody conjugated with Alexa Fluor 647 (red) was used at 1/1,000 dilution. DNA was stained with DAPI (blue).
Reference
This product was described and used in the following reference .
- Sekiguchi T., et al. (2004) A novel human nucleolar protein, Nop132, binds to the G proteins, RRAG A/C/D. J Biol Chem. 279: 8343-50. PubMed 14660641 WB
Aliases for RRAGC Gene
