Anti-MMAE, Mouse-Mono(AB12401)

Cat. No.: FNK-PP-AB12401-0R

Size: 100μl

Storage: -20℃

Label: HRP

Host Species: Mouse

Application: ELISA

Purification: Protein A/G Affinity Purified

Label: Unlabeled

Ig Class: IgG


Description:
 

Monomethyl auristatin E (MMAE) is a potent cytotoxic agent used as a payload in antibody-drug conjugates (ADCs) for targeted cancer therapy. It is conjugated to monoclonal antibodies, enabling selective delivery to cancer cells, where it exerts its toxic effects to induce cell death. The high potency of MMAE makes it an effective component in ADCbased cancer treatment.

Specifications:

MMAE: Monomethyl auristatin E

Clone No. AB12401

Lot. B-1

Concentration: 1mg/mL

Origin: CHO recombinant antibody stable expression strain

Conjugate: HRP

Specificity: This antibody reacts specifically with MMAE.

Formulation: Physiological saline with 0.1% NaN3 as a preservative.


Application / Recommended Concentration: 

In order to obtain the best results, optimal working dilutions should be determined by each individual user.

Western Blot	Not yet tested
Non reducing Western Blot	Not yet tested
ELISA	Immobilize the Anti-MMAE Antibody at 2.5 Pg/mL (see Data section below for further details.)
Immunoprecipitation	Not yet tested
Supershift Assay	Not yet tested
Chromatin immunoprecipitation	Not yet tested
Immunohistochemistry	Not yet tested

Data (Anti-MMAE Antibody Bridging ELISA):


1. Anti-MMAE Coating conc. Difference

Immobilize DXd-Ab, Exatecan-BSA or Naked Ab at 2.5 Pg/mL. Add HRP-conjugated Anti-DXd/Exatecan Antibody (PP-AB12703-0R) diluted with 50% mouse plasma. Detection was performed using an HRP-based immunoassay.

2. Comparison between MMAE-Ab and Naked Ab
 

Immobilize the Anti-MMAE Antibody (PP-AB12401-0R) at 2.5 μg/mL. Add MMAE-Ab or Naked Ab diluted with 50% mouse plasma, and then add HRP-conjugated Anti-Human IgG. Detection was performed using an HRP-based immunoassay. Limit of Detection*1: 1.37 ng/mL Range of Quantification*2: 4.12-111 ng/mL

^*1 Limit of Detection: Mean of the blank + 2 *standard deviation.

^*2 Range of Quantification is applied using ULOQ (Upper Limit of Quantification) and LLOQ (Lower Limit of Quantification). The ULOQ is the highest concentration with a back-calculated value of 80%-120% and a coefficient of variation (CV) of less than 30%. The LLOQ is the lowest concentration with a back-calculated value of 75%-125% and a CV of less than 30%.

 

Notes:

Store at 2 - 8 ºC up to one month. For long-term storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in a frost-free freezer is not recommended.

Sodium azide may react with lead and copper plumbing to form explosive metal azides. Flush with large amounts of water during disposal.
 

FOR RESEARCH ONLY. NOT FOR USE IN HUMANS.