Anti-Importin α3 / KPNA4 / Qip1 antibody, rat monoclonal (3D10)
Product#: FNK-70-325
$303.44
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Anti-Importin α3 / KPNA4 / Qip1 antibody, rat monoclonal (3D10)
General information
| Cat. No. | ：FNK-70-325 |
| Size | ：200 ug |
| Antigen Species | ：Rabbit |
| Cross Reactivity | ：Chicken/Human/Rodents |
| Specificity | ：Reactive with human, simian, mouse, rat, hamster, canine and bovine importin a3. This antibody doesn’t recognize other importin a family including a 4. Storage: -20℃ (long period, -70℃) |
| Immunogen | ：Recombinant mouse importin a 3 /KPNA4/ Qip 1 (full length) |
| Isotype | ：Rat IgG2a, kappa |
| Application | ：Western blotting (250~500 fold dilution), ELISA, This antibody doesn’t work for immunostaining and immunoprecipitation. |
| Storage | ：-20℃. |
| Form | ：Purified monoclonal antibody (IgG) 1mg/ml in PBS, 50% glycerol, filter-sterilized |
| Data Link | ：Swiss-Prot D3DNM2 |
Description
Importin a proteins play a pivotal role in the import of proteins from the cytoplasm to the nucleus. Importin a proteins shuttle between nucleus and cytoplasm, bind nuclear localization signal (NLS)-bearing proteins, and mediate the protein import into the nucleus with importin b. Several importin a isotypes have been identified, each exhibiting differential recognition and nuclear transport, probably via preferential binding to a particular NLS. The importin a 3 (KPNA4, Qip1) is a member of the importin a family of proteins belonging to the Qip1 subfamily. The antibody was purified from the serum-free cultured medium of the hybridoma under mild conditions by proprietary chromatography processes.
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Fig.1 Detection of importin a 3 (58 kD) by Western blotting using the antibody 3D10. Sample is the total cell extract. lane1: HeLa (human) lane2: COS7 (simian) lane3: L929 (mouse) lane4: NRK (rat) lane5: BHK (hamster) lane6: MDBK (bovine) |
References
This antibody was produced and used in Ref.3 and 4.
- Yoneda Y “Nucleocytoplasmic protein traffic and its significance to cell function.” Review. Genes Cells 5: 777-787 (2000) PMID: 11029654
- Miyamoto Y et al “Differential modes of nuclear localization signal (NLS) recognition by three distinct classes of NLS receptors.”J Bio Chem 272:26375-26381 (1997) PMID: 9334211
- Sakaguchi N et al “Generation of a rat monoclonal antibody specific for importin alpha3/Qip1.” Hybrid Hybridomics 22: 397-400 (2003) PMID: 14683601
- Yasuhara N et al “Triggering neural differentiation of ES cells by subtype switching of importin-alpha.”Nat Cell Biol 9:72-79 (2007) PMID: 17159997
Aliases for KPNA4 Gene
