Anti-Human Apolipoprotein A-IV, Mouse Monoclonal, (A4-11G12)

Product#: BML002
$577.00
Availability:
Ships in 1-2 weeks

Anti-Human Apolipoprotein A-IV, Mouse Monoclonal, (A4-11G12)
 

DiagnoCine offers excellent APOLIPOPROTEIN antibodies to researchers studying human induced pluripotent stem cell, mitochondrial dysfunction, pathophysiology, genetic polymorphisms, metabolism, phospholipase A2 activity, cellular surface factor, angiotensin-converting enzyme, nitric oxide synthase activity, amyloidosis, epigenome/metabolome research, sialylation proteoforms, pathogenesis, and progression.

Human diseases include type 1 diabetes, Memory Deficit, hepatitis C virus, Alzheimer's Disease, Osteoporotic Fractures. endoplasmic reticulum stress, atherosclerotic lesions, cancer, multiple sclerosis, Toxic Hetero-oligomers, brain amyloid load, hypertriglyceridemic pancreatitis, Atherosclerosis, Schnauzers, coronary artery disease, psoriasis, cardiac amyloidosis, cardiovascular disease and hypertension, dementia, type 2 diabetes mellitus, toxicity in human neurons, cognitive impairment, atherothrombosis, familial hypobetalipoproteinemia, hyperlipidemia, Parkinson's disease, metabolic syndrome, coronary heart disease, and depression.

APOLIPOPROTEIN antibodies have excellent quality and this highly pure antibody can be adapted for Western Blots, ELISA, Immunohistochemistry, Immunofluorescence research with optimization. 


General information 

Cat. No. BML002
Size :50 µg 
Formulation 0.2 µm filtered PBSsolution containing 0.1% sodium azide
Label :Unlabeled
Specificity :This antibody has been selected for its ability to bind for human plasma apoA-IV and recombinant
    apoA-IVfrom CHO cells by western blot.
Class :IgG
Clone A4-11G12
Application ELISA, Western Blot 
Cross Reactivity Human
Antigen Human 
Host Animal Mouse
Purification PAPu
Storage :IgG in PBS solution are stable for twelve months from the date of receipt when stored at-80˚C.
   Avoid repeated freeze-thaw cycles.

Preparation

Produced in mice immunized with apolipoprotein A-IV (apo A-IV) purified from human plasma. Apo A-IV specific IgG was purified from mouse ascites fluid with a proteinA-Sepharose.


Additional Applications

Western Blot – This antibody can be used at 0.5 – 1.0 µg/mL with the appropriate secondary reagent to detect human plasma apo A-IV. The detection limit for purified apo A-IV and plasma sample is approximately 0.01 µg/lane and 0.1 µL, respectively, by SDS-PAGE and western blotting under reducing condition.

Sandwich ELISA – Biotinylated antibody (BML003) of this antibody can be used as a detection antibody in a human apo A-IV ELISA in combination with the monoclonal capture antibody (Catalog #BML001). In general, using plates coated with 100 µL/well of 5 µg/ml capture antibody (Catalog #BML001), in combination with 100 µL/well of 0.05 µg/ml detection antibody (Catalog #BML003), an ELISA for sample volumes of 100 µL can be obtained. Titrate each preparation of the serum sample for standard preparation to arrive at the most suitable dose range. For this antibody pair, a two-fold dilution series starting at 200 ng/mL is suggested.

Optimal dilutions should be determined by each laboratory for each application.

 

Aliases for APOA4 Gene

  • Apolipoprotein A4 2 3 4 5
  • Apolipoprotein A-IV 2 3 4
  • Apo-AIV 3 4
  • ApoA-IV 3 4
  • APOA4 5


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