:Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
:PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol.
Application
Applications
Species
Dilution
Positive Tissue
WB
Human, Mouse, Rat
1: 250-1: 500
liver, lung
IHC
Human
1: 1500-1: 3000
ovarian cancer, breast cancer, kidney, placenta
IF
Human
1: 500-1: 1000
lung cancer, placenta, para-cancer liver
Description
Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. Through the extensive study of groEL, HSP60’s bacterial homolog,
Western blot analysis of HSP60 (GB112464)
at dilution of 1: 500
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Mouse lung tissue lysate
Lane 6: Rat liver tissue lysate
Lane 7: Rat lung tissue lysate
Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using HSP60 (GB112464) at dilution of 1: 2000
Immunohistochemistry analysis of paraffin-embedded human breast cancer using HSP60 (GB112464) at dilution of 1: 2000
Immunohistochemistry analysis of paraffin-embedded human kidney using HSP60 (GB112464) at dilution of 1: 2000
Immunohistochemistry analysis of paraffin-embedded human placenta using HSP60 (GB112464) at dilution of 1: 2000
Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using HSP60 (GB112464) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed human para-cancer liver using HSP60 (GB112464) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed human placenta using HSP60 (GB112464) at dilution of 1: 1000