:Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
:PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol.
Application
Applications
Species
Dilution
Positive Tissue
WB
Human
1: 350-1: 700
HUVEC
Description
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Western blot analysis of HLA E (GB112510) at dilution of 1: 700