Anti-FcεR1α (human IgE receptor)monoclonal antibody (CRA2)

Product#: 72-005
$375.00
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Anti-FcεR1α (human IgE receptor)monoclonal antibody (CRA2) 
 

DiagnoCine offers excellent Anti-FcεR1α | Human IgE (Immunoglobin E) receptor antibodies  to researchers studying Allergic rhinitis treatment, Intranasal antihistamines, Intranasal corticosteroids, Leukotrienes, Subcutaneous immunotherapy, and Mechanisms & Treatment of Allergy.

Human diseases include Seasonal rhinitis, Atopic dermatitis, Anaphylaxis, Asthma, Dermatographia, and Physical Urticaria.

Anti-FcεR1α | Human IgE (Immunoglobin E) receptor antibody has excellent quality and this highly pure antibody can be adapted for Western Blots, ELISA, Immunohistochemistry, Immunofluorescence research with optimization.  


General Information

Cat. No. :72-005
Size :100 ug
Antigen Species Human
Host Species Mouse
Cross Reactivity Human 
Clone :AER24
Epitope Amino acids 85-172 of FcεR1α (Ref 3)
Isotype IgG1 (κ)
Application  
  1. Western blotting (~1ug/ml) (Ref 3, 4)
  2. FACS (Ref 3, 4, 5)
  3. Immunohistochemistry and immunocytochemistry (Ref 6) and CRA2 antibodies (Ref 3)
  4. Inhibition of binding of IgE with FcεR1α. Titration of IgE-bound fraction of the FcεR1α using CRA1 and CRA2 antibodies (Ref 3) 
Storage Shipped at 4℃ or -20℃ and stored at -20℃
Form Purified monoclonal antibody (IgG) 1mg/ml in PBS (pH 7.4), 50% glycerol, filter-sterilized, azide-free
Data Link UniProtKB/Swiss-Prot P12319 (FCERA_HUMAN)
 

Description 

FcεR1α is a subunit of the high-affinity receptor for IgE to which IgE directly binds. FcεR1α is a tetrameric complex consisting of one α, one β, and two γ subunits. The latter two are required for signal transduction activity. The FcεR1 complex plays an important role in triggering allergic responses.

The CRA2 (AER24) monoclonal antibody reacts with the FcεR1α subunit on a region that overlaps the region of the IgE binding site, thus it competes with IgE for the receptor binding. Since the CRA1 (AER37) monoclonal antibody reacts with the site different from the IgE binding site on FcεR1α, it does not compete with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound FcεR1α.

This product is the IgG fraction purified from the serum-free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions.

 


Figure: FACS analysis of CHO/αβγ cells (1x105) with CRA1 and CRA2 antibodies by indirect-immunostaining using FITC-labeled secondary antibody.
72-007_e.jpg
 


References 

  1. Ra C et al “A macrophage Fc gamma receptor and the mast cell receptor for IgE share an identical subunit”Nature 341:752-754 (1989) PMID: 2529442
  2. Hakimi J et al “The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding” J Biol Chem 265:22079-22089 (1990) PMID: 2148316
  3. Takai T et al “Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions” Biosci Biotechnol Biochem 64:1856-1867(2000) PMID: 11055388
  4. Takai T et al “Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli ”Biosci Biotechnol Biochem 65:79-85 (2001) PMID: 11272849
  5. Hasegawa S et al. “Functional Expression of the High Affinity Receptor for IgE (FceRI) in Human Platelets and Its’ Intracellular Expression in Human Megakaryocytes” Blood 93: 2543-2551 (1999) PMID: 10194433 
  6. Goto T et al. “ Enhanced expression of the high-affinity receptor for IgE (Fc(epsilon)RI) associated with decreased numbers of Langerhans cells in the lesional epidermis of atopic dermatitis” J Dermatol Sci. 27:156-61 (2001) PMID: 11641054
*This product was described and used in references 3-6.



Aliases for FCER1A Gene

  • Fc Fragment Of IgE Receptor Ia 2 3 5
  • Fc Fragment Of IgE, High Affinity I, Receptor For; Alpha Polypeptide 2 3
  • High Affinity Immunoglobulin Epsilon Receptor Subunit Alpha 3 4
  • IgE Fc Receptor Subunit Alpha 3 4
  • Fc Epsilon Receptor Ia 2 3
  • Fc-Epsilon RI-Alpha 3 4
  • FCE1A 3 4
  • FcERI 3 4
  • Immunoglobulin E Receptor, High-Affinity, Of Mast Cells, Alpha Polypeptide 3
  • High Affinity Immunoglobulin Epsilon Receptor Alpha-Subunit 3
  • Fc IgE Receptor, Alpha Polypeptide 3
  • Fc Epsilon RI Alpha-Chain 3
  • FCER1A 5
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