Anti-E2F1 p-Ser364 antibody, monoclonal (#2)
Anti-E2F1 p-Ser364 antibody, monoclonal (#2)
General Information
| Cat. No. | ：FNK-71-151 |
| Size | ：50 ug |
| Antigen | ：Human |
| Host Species | ：Mouse |
| Cross Reactivity | ：Human |
| Label | ：Unlabeled |
| Clone | ：2 |
| Product | ：Mouse monoclonal antibody (clone #2) specific to the human E2F1 protein phosphorylated at Ser364. Produced in serum-free medium and purified under mild conditions |
| Immunogen | ：A synthetic peptide corresponding to a sequence of human E2F1 protein including and surrounding phospho-Ser364 |
| Reactivity | ：Human E2F1 protein phosphorylated at Ser364. Not tested with other species. |
| Form | ：Purified IgG 1 mg/ml in PBS(-), 50% glycerol |
| Isotype | ：Mouse IgG2b (κ) |
| Application |
|
| Storage | ：-20℃ (long period; -70℃) |
| Data Link | ：UniProtKB/Swiss-Prot Q01094 (E2F1_HUMAN) |
Description
E2F1 is a member of E2F group of proteins that share common structural and functional domains and plays a major role during the G1/S transition in the mammalian cell cycle as a transcriptional factor (1). E2F1 is regulated during cell cycle progression. It is phosphorylated at Ser364 by Chk2 kinase in response to DNA damage, stabilized, mobilized to nucleus and activated as a transcription factor (2). It induces apoptosis by activating transcription of the p53 homolog, p73 (3). E2F1 protein consists of 437 amino acids with a molecular mass of 46 kDa.
Figure. Identification of E2F1 protein phosphorylated at p-Ser364 with monoclonal antibody (#2)
MCF cells were grown in the absence (lane 1) or in the presence of etoposide at 10 μM for 16 h (lanes 2 & 3). Crude lysates were 1 2 3 (kD) 100 60 50 40 80 ←E2F1 p-S364 IP followed by WB WB prepared and analyzed by Western blotting (lane 3) with the antibody #2 or immunoprecipitated by pantropic anti-E2F1 antibody followed by Western blotting with the antibody #2.
References
- Trimarchi JM & Lees JA ”Sibling rivalry in the E2F family” Nat Rev Mol Cell Biol 3:11-20(2002) PMID: 11823794
- Stevens C et al “Chk2 activates E2F-1 in response to DNA damage”Nat Cell Bio1 5:401-409 (2003) PMID: 12717439
- Irwin M et al “Role for the p53 homologue p73 in E2F-1- induced apoptosis”Nature 407:645-648 (2000) PMID: 11034215
Aliases for E2F1 Gene
- E2F Transcription Factor 1 2 3 5
- Retinoblastoma-Associated Protein 1 3 4
- Retinoblastoma-Binding Protein 3 3 4
- Transcription Factor E2F1 3 4
- PRB-Binding Protein E2F-1 3 4
- RBAP-1 3 4
- RBBP-3 3 4
- E2F-1 3 4
- RBBP3 3 4
- RBP3 2 3
- PBR3 3 4
- RBAP1 3
- E2F1 5
