Anti-Asymmetric Dimethylarginine of TLS/FUS (R216, R218), Human, Mouse-mono (20 ug)
Anti-Asymmetric Dimethylarginine of TLS/FUS (R216, R218), Human, Mouse-mono
General information
| Cat. No. | ：FNK-FDV-0006 (25 ug) |
| Size | ：20 ug or 100 ug (FDV-0007) |
| Antigen Species | ：Human |
| Host Species | ：Mouse |
| Clone No. | ：2B12 |
| Specificity | ：This antibody recognizes asymmetric dimethylarginine (R216, R218) of TLS/FUS. This antibody does not cross-reacts against symmetric dimethylarginine of TLS/FUS, and non-methylated TLS/FUS. |
| Immunogen | ：Synthetic CGGRme2aGRme2aGGSG |
| Subclass | ：IgG |
| Application | ：WB（1:500 – 1:2000）, IP |
| Purification | ：Protein G Purified |
| Concentration | ：0.5 mg/ml |
| Cross Reactivity | ：Human, Mouse, Hamster (CHO cell) – Not yet tested in other species. |
| Lot Number | ：See vial label |
| TLS | ：Translocated in LipoSarcoma |
| FUS | ：Fused in Sarcoma |
| Storage Buffer | ：Phosphate buffered saline, pH7.2, no preservative added |
| Storage | ：Store below -20℃. Avoid repeated freeze-thaw cycles. |
| Intended Use | ：For Research Use Only. Not for diagnostic or other use. |
Description
This antibody recognizes asymmetric dimethylarginine (R216, R218) of TLS/FUS. It does not cross-reacts against symmetric dimethylarginine of TLS/FUS, and non -methylated TLS /FUS.
Arginine methylation is one posttranslational modification. Arginine can be methylated once (monomethylated arginine) or twice (dimethylated arginine). Dimethylation can be further categorized as symmetric (me2s) or asymmetric (me2a). TLS/FUS (Translocated in LipoSarcoma/Fused in Sarcoma) is known as one of the causative genes for familial amyotrophic lateral sclerosis. Specifically, it is reported that four arginine residues in TLS/FUS (R216, R218, R242, and R394) are constitutively dimethylated by protein arginine methyltransferases. However, the function of methylated TLS/FUS is still unclear.
Application
 |
Lane1 Recombinant GST-TLS/FUS methylated using PRMT1 in the presence of SAM (20 µM) in vitro. Lane2 Cell extract from CHO cells immunoprecipitated with rabbit polyclonal anti-TLS/FUS antibody (Bethyl Laboratories, #A300-302A). ※It is hard to detect the specific band for methylated TLS/FUS with crude lysate sample. We recommend to do immunoprecipitation using polyclonal anti-TLS/FUS antibody prior to WB. |
Arginine methylation of TLS/FUS
Type of arginine methylation
Reference
- Jun M, et al. Sequestration of PRMT1 and Nd1-L mRNA into ALS-linked FUS mutant R521C-positive aggregates contributes to neurite degeneration upon oxidative stress. Sci Rep. (2017) 7: 40474.
