Anti IZUMO1 antibody, rat monoclonal (#125)
Product#: FNK-73-045
$346.79
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Anti IZUMO1 antibody, rat monoclonal (#125)
General information
| Cat. No. | ：FNK-73-045 |
| Size | ：100 ug |
| Host Species | ：Rat |
| Cross Reactivity | ：Human/Mouse |
| Reactivity | ：Mouse. Not tested with other species. |
| Immunogen | ：Mouse sperm |
| Isotype | ：Rat IgG |
| Application | ：Western blotting (1/1,000 dilution)), Immunoprecipitation (1/100 dilution), Immunofluorescence staining (1/250 dilution)) |
| Storage | ：Shipped at 4℃ or at -20℃. Upon arrival, spin-down, aliquot and store at -20℃.. |
| Form | ：Purified IgG 1 mg/ml in PBS, 50% glycerol. Filter-sterilized. Azide- and carrier- free |
| Data Link | ：UniProtKB Q9D9J7 (mouse IZUMO1) |
Key words
Acrosome reaction, Membrane fusion, Protein trafficking, IZUMO1, Sperm–egg fusion
Function
Essential sperm cell-surface protein required for fertilization by acting as a ligand for FOLR4/JUNO receptor on egg. The IZUMO1:FOLR4/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'
Molecular mass
44,885 Da with 307 amino acids (mouse). Post-translational modification; Processing of N-terminal signal peptide with 21 amino acids. N-Glycosylation and phosphorylation.
Expression Testis (low level) and sperm (high level). Not expressed in other organs.
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Fig.1 Identification of IZUMO1 protein in sperm lysate of wild type mouse but not in that of IZUMO1 knock-out mouse by western blotting with anti-IZUMO1 monoclonal antibody ‘#125). Proteins in the lysates (10μg) were separated on SDS-PAGE, electro-blotted to PVDF membrane and incubated with anti-IZUMO1 antibody (#125) at 1/1,000 dilution. Lane 1; Sperm lysate from wild-type mouse Lane 2; Sperm lysate from IZUMO1 knock-out mouse |
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Fig.2 Analysis of IZUMO1 protein in the lysates of mouse testis and sperm by western blotting with anti-IZUMO1 antibody (#125). Proteins in the lysates were separated on SDS-PAGE (10~20% gradient gel) electro-blotted to PVDF membrane and incubated with anti-IZUMO1 antibody (#125) at 1/1,000 dilution. As the second antibody, goat anti-rat IgG antibody conjugated with HRP (Abcam; ab97057) was used at 1/10,000 |
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Fig.3 Immunoprecipitation of IZUMO1 protein from lysate of mouse sperm by using anti-IZUMO1 antibody (#125). Mouse sperm lysate (1.5 mg protein in 1ml) solubilyzed with 1% Brij 97 was incubated with anti-IZUMO1 antibody (10 μ g) and protein G beads. Proteins eluted from the beads were analyzed by western blotting using the same antibody. Lane 1; Negative control without the antibody Lane2; Anti-IZUMO1 antibody (#125) |
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Fig.4 Immunofluorescence stainig of IZUMO1 protein in mouse spermatozoa with anti-IZUMO1 antibody (#125). Spermatozoa was spotted onto slide, air dried, fixed with ice-cold 100% ethanol and rinsed with PBS. The slide was incubated with anti-IZUMO1 antibody (#125) at 4 μg/ml. The slide was then incubated with Alexa Fluor 488-conjugated anti-rat IgG. The stained cells were observed under a fluorescence microscope. |
Reference
This antibody was used in the following publications.
- Inoue N. et al. (2010) Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse. PLoS One. 2010 Apr 22;5(4):e10301. PubMed 20421979 WB, IP, IF. Open access
- Ikawa M. et al. (2011). Calsperin is a testis-specific chaperone required for sperm fertility. J Biol Chem. 286: 5639-46. PubMed 21131354 WB. Open access
- Satouh Y. et al. (2012) Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1. J Cell Sci. 125: 4985-90. PubMed 22946049 WB. Open access
- Inoue N. et al. (2013). Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development. 140: 3221-9. PubMed 23824580 WB, IF. Open access
Aliases for IZUMO1 Gene
- Izumo Sperm-Egg Fusion 1 2 3 5
- Oocyte Binding/Fusion Factor 2 3 4
- OBF 2 3 4
- Izumo Sperm-Egg Fusion Protein 1 3 4
- Sperm-Specific Protein Izumo 3 4
- IZUMO 2 3
- MGC34799 2
- IZUMO1 5
