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Anti HBsAg Pre-S1, Monoclonal Antibodies, Mono-2

Product#: FNK-BCL-AB-02

$990.77

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Anti HBsAg Pre-S1, Monoclonal Antibodies, Mono-2

General information

Cat. No.	：FNK-BCL-AB-02
Size	：100µg
Source	：Mouse ascitic fluid, Monoclonal
Appearance	：Liquid form（saline containing biochemically inactive preservative）
Concentration	：1 mg/mL
Antigen	：HBsAg L-protein
Antigen specificity	：Bind only to Pre-S1 domain and not to S or Pre-S2 domains
Purification	：Affinity by Protein G
Isotype	：IgG1
Storage	：4°C (for long term storage at -20℃ avoid freeze and saw)
Usage	：ELISA (0.22 ug/mL or to be tested by user)、Western blot (50 to 200 ng/mL)

Introduction

HBsAg is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection. Beacle has over a decade of R&D history on HBsAg and related materials. In this page we introduce HBsAg-related antibodies. All antibodies listed here are good for western blot and ELISA.

Description

Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. So called HBsAg is composed of either S-protein alone or a mixture of S- and M-proteins. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection. Pre-S1 region is known to have variation of amino acid sequence depending on genotypes. The present products are monoclonal antibody against Pre-S1 domain.

Anti HBsAg Pre-S1, Monoclonal Antibodies

Mouse monoclonal antibody against Pre-S1 reagion. Two types of anti-Pre-S1 monoclonal antibodies are available. Both products can be used for Western blotting. A sandwich ELISA system can be established by using these two antibodies or with below polyclonal antibody.

	Fig. 1: Specificity of the antibody recognition. All antibodies bind to L antigen of genotype A, B, C and D.
	Fig. 2: Western Blotting for HBsAg. Sample: HBsAg-L protein (BCL-AGC-01, 5ng) Primary Antibody: Mono-1,-2 (100ng/mL) 2nd antibody: anti-mouse rabbit-HRP Detection: ECL substrate Monomer and Dimer bands are detected
	Fig. 3: Western Blotting for HBsAg, L-protein Sample: purified HBsAg from human serum 500ng Primary Antibody: Mono-1 (100ng/mL) 2nd antibody: anti-mouse rabbit-HRP Detection: ECL substrate L-protein is detected in human HBsAg.