Ant-Staphylococcus aureus Enterotoxin A/B.Mouse-Mono, (sa-01)

Cat.No: FNK-64-040

Size: 100 μg

Storage: -20℃

Concentration: 1.0 mg/ml

Buffer: PBS-with 50% glycerol

Purity: Purified IgG fraction with protein A from hybridoma cell culture medium. 

Immunogen: Crude supernatant of Staplylococcus aureus. 

Isotype: Mouse IgG2a

Reactivity: Enterotoxin A and B of Staplylococcus aureus. Reactvitiy with other types of the enterotoxins has not been tested. 
 

Application

1. Western blotting (1/500~1/1,000)

2. ELISA (assay dependent)

3. Immunochromatography (assay dependent)

This antibody is used for detecting food-poisoning S.aureus. 
 

Background

Over 20 serologically distinct staphylococcal enterotoxins (SE) have been described that include SEs A through V and toxic shock syndrome toxin-1 (TSST1). SEA and SEB are the best characterized and are also regarded as super antigen. SEA, SED and SEE share 70–90% sequence homology, while only 40– 60% with SEB, SEC and TSST-1. Their mature length is approximately 220– 240 amino acids, depending on the toxin, and their molecular size is on average 27kD to 30kD and have significant sequence variability, but when folded have similar three-dimensional structures.

Data Link

UniProtKB: P0A0L2 (ETXA_STAAU), P01552 (ETXB_STAAU)


Please note: All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. NOT FOR MILITARY USE.

Data Images

64-040 Anti-Staphylococcus aureus Enterotoxin A/B antibody, mouse monoclonal (sa01)



Fig.1. Detection of S. aureus enterotoxins by Western blotting with monoclonal antibody (MAb sa-01)

1. Culture medium of S. aureus

2. Purified S. aureus enterotoxin A (Sigma-Aldrich)

3. Purified S. aureus enterotoxin B (Sigma-Aldrich)

The molecular masses of enterotoxin A and B are 30 kDa and 29 kDa, respectively.




Fig 2. Reactivity of the MAb (sa-01) with various food poisoning bacteria in immunochromatographic strip test

Mouse MAb (BioAcademia sa-02) was coated onto a specific area (test line) of a nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with the MAb (sa-01) conjugated with colloidal gold. The strip was soaked and reacted with the mixture fluid. (1) S. aureus, (2) Escherichia coli O157:H7, (3) E. coli K12, (4) Salmonella Enteritidis, (5) Campylobacter jejuni, (6) Vibrio parahaemolyticus, (7) PBS. MAb (sa-01) reacted with S. aureus, but not with other food poisoning bacteria.


Fig.3. Titration of antibody reactivity of MAb (sa-01) by indirect ELISA using crude extract of S.aureus

The wells of plate were coated with crude extract of S. aureus (100 µl, 1 μg/ml). After blocking with 5% skim milk, 100 µl of antibody at the indicated dilution was added to the each well. HRP-conjugate
goat anti-mouse IgG (100 μ l, x2000 dilution) was added. Color was developed with orthophenylenediamine as substrate. Optical density (OD) measured at 490nm.

Table 1. Reactvity of MAb (sa-01) with various food posioning bacteria
 

	ELISA	WB
Staphylococcus aureus (NBRC 15306)	+	28K
Other 5 Isolated strains	+
Camplyobacter jejuni	-	-
Salmonella Enteritidis	-	-
Escherichia coli	-	-
E.coli O157:H7	-
Clostridium perfringens	-
Bacillus cereus	-	-
S.aureus enterotoxin A (SEA)	+	28K
S.aureus enterotoxin B (SEB)	+	28K
S.aureus enterotoxin (Set A, B, C, D, E)	+

SEA, SEB: Sigma-Aldrich, Inc.
Ridascreen set A, B, C, D, E postitive contral (R-Biopharm AG)