Amido Black 10B Staining Solution
Cat No.: DCP-AB10B1X
Size: 500 mL
Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Amido Black 10B (CAS 1064-48-8), a diazo dye optimized for protein detection and forensic applications, is now available in a methanol-free, acetic acid-based formulation. Designed for laboratories prioritizing safety and stability, this solution eliminates flammable alcohols while maintaining high sensitivity for protein visualization in electrophoresis, Western blotting, and bloodstain enhancement. This acetic acid-based variant of Amido Black 10B provides a safer alternative to traditional methanol-containing formulations, reducing health risks associated with volatile organic compounds. The solution retains the dye’s signature blue-black staining for proteins at concentrations as low as 50 ng/band on nitrocellulose or PVDF membranes. Its acidic composition (7% acetic acid) ensures rapid dye penetration and minimal background interference, making it ideal for high-resolution applications in proteomics and forensic science.
Applications:
1. Electrophoresis and Western Blotting
- Protein Detection: Stains total proteins in SDS-PAGE gels and Western blots with 0.1–0.2% (w/v) Amido Black 10B dissolved in 7% acetic acid.
- Downstream Compatibility: Fully reversible staining allows for subsequent mass spectrometry or immunodetection workflows. Destaining with 5–7% acetic acid removes >95% background within 30 minutes.
- Agarose Gel Optimization: Demonstrated efficacy in 5–15 minute staining protocols for agarose gels, with destaining completed in 2–24 hours (vs. Coomassie Brilliant Blue’s overnight requirements).
2. Forensic Bloodstain Enhancement
- Latent Blood Visualization: Reacts with hemoglobin and serum albumin on porous/non-porous surfaces, producing permanent blue-black patterns. Compatible with DNA preservation protocols, unlike luminol-based methods.
- Reduced Background: Acetic acid minimizes nonspecific binding on semi-porous materials (e.g., wood, fabric), improving contrast compared to methanol blends.
3. Histological Staining
- Collagen Specificity: Binds selectively to collagen fibers in tissue sections, enabling differential visualization of connective tissues in 10–15 minute protocols.
Advantages:
- Reduced Flammability: Eliminates methanol (flash point 12°C), complying with OSHA safety standards.
- Extended Storage: Acetic acid’s lower volatility prevents concentration shifts, maintaining pH stability.
- Material Compatibility: Safe for painted surfaces and polymers degraded by alcohols.
Composition:
| Ingredients |
Concentration |
| Amido Black 10B |
2% |
| Acetic Acid |
7% |
Quality Control:
- Appearance: Blue-black solution
- pH: 2.5
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Store at 4 °C away from bright light.
- Shelf life is 6 months.
- Use before expiry date given on the product label.
Disposal:
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
