DiagnoCine Precision® ELISA Kit Reagents included
- SigMax 1Ab-TBS: add to ELISA protocol when increased signal intensity is required
- Universal Blocking Buffer: use when background interference compromises signal-to-noise ratio
- Protease Inhibitor Cocktail: incorporate during the sample preparation phase or add directly to the ELISA protocol as required to prevent protein degradation.
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African Swine Fever Antibody (ASF-Ab)
Cat.No.: JNB-JL47607
Size: 96TSpecies: PigAssay Type: IndirectSensitivity: Detection Range: QualitativeAlternative Names: ASF-AbResearch Areas: MicrobiologyAssay Time: 1h45mAbbreviation: ASF-AbTest Principle: This assay employs the indirect method enzyme immunoassay technique. The micro ELISA plate provided in this kit has been pre-coated with African Swine Fever antigen. Add Sample, Positive Control, Negative Control, and HRP-antibody by turn, with incubation and washing. TMB Substrate is added for color development, and TMB will appear blue in color catalyzed by HRP and to a final yellow color in reaction to acid. The intensity of the color is positive to the Pig ASF-Ab in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450nm. Determine the sample's positive or negative status by measuring the OD value.Specificity: This kit recognizes Pig ASF-Ab in samples. No significant cross-reactivity or interference between Pig ASF-Ab and analogues was observed.Recovery rate: 80%-120%Precision: Intra-CV and Inter-CV <10% Shipping: Ice Bag