5-Layer Cell Culture Flask, Plug Seal Cap, Straight Neck, TC, sterile 1/pk, 8/cs
5-Layer Cell Culture Flask
Cat. No. NST-731001
Description
Product features
- Vacuum plasma TC treatment for the best cell attachment
- Sterilized by E-Beam
- Non-Pyrogenic, DNase/RNase FREE
- Both plug seal cap and vent cap are available
- Growth area: 870cm2
- TC treatment for better cell attachment
- Sterilized by E-Beam, non-pyrogenic
- Tips: To avoid foams and bubbles causing the media in upper layer flowing into the bottom layer, users should be gently when operating the multi-layer flasks.
| Product ID | Cell Growth area (cm2) | Cap Style | Recommended Medium Volume (mL) | Treat | Packing Unit | |
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NST-731001 | 870 | Sealed Cap | 100-150 | Yes | 1/pk, 8/cs |
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NST-731002 | 870 | Vent Cap | 100-150 | Yes | 1/pk, 8/cs |
| Adding Media and Preparing Cell Suspension: | |
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1. Mix cell suspension with medium: Prepare cell suspension of required concentration in a container and mix. Recommended volume is about 30- 50 mL per layer. 2. Add the mixed liquid into the Multi-layer Flask slowly with serological pipettes. To avoid foams and bubbles, allow liquid stream to flow along the slope of the Multi-layer Flask. (Save a little liquid in pipette for each dosing). Tips: A 10 mL pipette allows media to be dispensed at the bottom of the vessel. A 25 mL pipette allows media to be dispensed just past the NEST Logo. |
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3. Hold the Multi-layer Flask up right with the Logo facing you and tilt clockwise to a 45° angle on a flat work surface to partition the liquid into each layer. 4. While holding the Multi-layer Flask at a 45° angle, gently lay it flat onto the work surface with logo facing up. |
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5. After placing the Multi-layer Flask flat on a work surface, gently rock back and forth and side-to-side to distribute cells evenly onto culture surfaces. Tips: Take care to avoid foaming of medium, and not to spill liquid from each layer. 6. Repeat Step 3 to put the flask quickly and slightly into the incubator. Then, lay it flat as shown in Step 4. |
| Media Removal: You may choose to either aspirate or pour the media from Multi-layer Flask |
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7. Aspirating method: To aspirate or remove media, tilt Multi-layer Flask, with the NEST Logo facing you, counter-clock wise to a 45° angle while inverting the Multi-Flask toward you. Then, tilt Multi-layer Flask to the right, continuing to aspirate all residual media. |
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8. Pouring method: With Logo facing you, pour spent media from Multi-layer Flask Tips: Aspirate media using a NEST 2mL or 10mL aspirating pipette. |
| Cell Harvesting: | |
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10. Neutralize with inactivating solution and mix following Steps 3-4. Gently swirl to dislodge cells completely. 11. Follow Step 7 "Aspirating Method” protocol and collect cell suspension using a NEST 10mL serological pipet. 12. Follow Step 8 “Pouring Method”. Pour detached cell suspension into a NEST conical tube. 13. Rinse with additional wash buffer as needed. |
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Application
- Cell biology research
- Cytology research in animal and plant and basic medical school
- Small-scale cell culture, cell screening
- Cell threapy direction research
- Stem cell research
- Tissue culture
- Biochemistry, medicine, microbiology
- Genetic engineering, antibody engineering drugs, vaccines, etc
- Microplate chromogenic reaction plate use
| Working Volume Range: | >5mL per layer for dissociating, >30mL per layer for cell expansion |
| Molded-In Graduations: | 0 to 50mL per layer in 10mL increments |
| Graduation Accuracy: | 10% |
| Cap Vent Membrane: | 0.22um hydrophobic membrane |
| Cell Growth Surface: | tissue culture-treated, optically clear |
Remark
- Such products are provided with Treated, Non-Treated products, applicable for adherent cells or suspension cell culture
