5% Hydrogen Peroxide Solution (H2O2)
Size: 500 mL
Description:
Our 5% H
2O
2 Surface Decontamination Solution is specifically formulated for molecular biology laboratories conducting RNA work. This powerful yet safe oxidizing agent effectively denatures and inactivates ribonucleases (RNases) on all common laboratory surfaces, providing reliable protection for your valuable RNA samples.
RNases are notoriously resilient enzymes that can withstand autoclaving and survive on surfaces for extended periods. Our 5% hydrogen peroxide solution leverages oxidative chemistry to denature RNase proteins, rendering them permanently inactive. Unlike reversible inhibition methods, H
2O
2 causes irreversible oxidation-induced conformational changes in RNase structure, ensuring complete deactivation.
Applications:
- Daily decontamination of laboratory benchtops and workspaces.
- Weekly cleaning of pipettes and tube racks.
- Decontamination of electrophoresis equipment prior to RNA work.
- Treatment of polycarbonate and polystyrene materials.
- Surface preparation in designated RNase-free work areas.
Instructions:
- Apply: Wipe the laboratory surface thoroughly with a paper towel saturated with the 5% H2O2 solution, ensuring complete coverage of the area.
- Wait: Leave the surface wet for 10-15 minutes to allow complete RNase deactivation. This contact time ensures thorough oxidation of RNase proteins.
- Rinse: After the 15-minute contact period, rinse the treated surface with DEPC-treated ultrapure water to remove residual hydrogen peroxide. This step is critical to prevent any potential interference with downstream RNA applications.
Technical Specifications:
| Parameter |
Specification |
| Active Ingredient |
Hydrogen Peroxide (H2O2) |
| Concentration |
5% (50 g/L) |
| Contact Time |
15 minutes (minimum 10-15 min) |
| Post-Treatment |
Rinse with DEPC-treated RNase-free water |
| Compatible Surfaces |
Glass, plastic, metal, polycarbonate, polystyrene |
| Shelf Life |
1 year |
| Storage |
Cool, dark place for maximum stability |
* H2O2 at 5% is stronger than the typical 3% household solution—handle with gloves and eye protection.
* All DiagnoCine Precision Sterile media are filtered-sterilized with a 0.1-micron filtration two times and 0.04 micron once. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
