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49.5% Acrylamide : Bisacrylamide Solution (32:1)

Product#: DCP-ABS49.5X
$174.90
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
FluxMPS™ Microfluidics Suitable Reagent
ISO 13485 Certified Manufacturing

FluxMPS™ 49.5% Acrylamide : Bisacrylamide Solution (32:1)

Microfluidics Suitable, dual-stage ultra-filtered (0.1 µm x1 + 0.04 µm x1) 49.5% acrylamide:bisacrylamide (32:1) liquid solution engineered for high-resolution protein and nucleic acid electrophoresis. Ready-to-use, non-sterile grade formulation eliminates hazardous powdered-acrylamide handling while supporting on-chip and lab-on-chip gel-casting workflows.

  • 49.5% total acrylamide (T) with 3% crosslinker (C) at a 32:1 acrylamide:bisacrylamide ratio, producing larger pore-size gels than standard 29:1 formulations
  • Ready-to-use liquid solution eliminates the need to weigh and handle hazardous powdered acrylamide
  • Dual-stage filtration: 0.1 µm membrane once, 0.04 µm membrane once, Non-Sterile grade
  • No DNase activity detected after 18-hour incubation with plasmid DNA at room temperature
  • No RNase activity detected after 18-hour incubation with ribosomal RNA at room temperature
  • Clear solution, pH 6 - 7
  • Manufactured under an ISO 13485-certified quality management system; final packaging, QA and customization performed at the Diagnocine R&D and Quality Testing Center, Totowa, New Jersey, USA
  • Custom concentrations, tracking dyes, and pH modifications available on request
SKU: DCP-ABS49.5X Molecular Biology Reagents UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
49.5% Acrylamide:Bisacrylamide Solution (32:1) — Liquid, 500 mL
  • Acrylamide:Bisacrylamide Ratio32:1
  • Total Concentration (T)49.5%
  • Crosslinker (C)3%
  • pH6 - 7
  • AppearanceClear solution
  • Filtration0.1 µm x1 + 0.04 µm x1
  • DNase ActivityNone detected
  • RNase ActivityNone detected
  • Storage4°C
  • Shelf Life2 years from date of manufacture, unopened
ISO 13485:2016 Dual-Stage Filtered RUO
Why FluxMPS™

Engineered where standard acrylamide solutions fall short

Conventional single-pass filtered acrylamide:bisacrylamide solutions carry particulate loads and inconsistent ratio control that show up as streaking, uneven pore structure, and batch-to-batch variability. FluxMPS™ addresses each failure mode directly.

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Dual-stage filtered clarity

Sequential 0.1 µm and 0.04 µm filtration removes particulates and microaggregates that a single-pass 0.22 µm filter leaves behind, supporting clean gel casting.

target

Precision 32:1 formulation

Consistent 49.5% total monomer (T) and 3% crosslinker (C) ratio, batch after batch, so pore size and gel behavior remain predictable across experiments.

science

Nuclease-tested purity

No DNase or RNase activity detected after 18-hour incubation with plasmid DNA and ribosomal RNA, respectively, protecting nucleic acid samples run on these gels.

visibility

Low background for imaging

Clear, particulate-reduced solution supports clean band visualization during electrophoretic and imaging workflows.

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Ready-to-use safety

Pre-mixed liquid formulation eliminates the need to weigh and handle hazardous powdered acrylamide in the lab.

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Customization on demand

Alternate concentrations, tracking dye additions, and pH modifications are available — contact support@diagnocine.com.

Purity Architecture

Dual-stage filtration system

This solution is processed through a validated 0.1 µm prefilter followed by a 0.04 µm final filter, a Non-Sterile grade dual-stage train that removes particulates and microaggregates well below a conventional 0.22 µm single-pass filter.

  1. 1

    0.1 µm Prefiltration

    Removes large particulates and process residues, protecting the downstream 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration — Polish

    Sub-micron polishing pass removes fine particulates and microaggregates, yielding a clear, ready-to-use solution suitable for high-resolution gel casting.

Filtration architecture

A dedicated prefilter protects the final filter cartridge in a two-stage, single-pair train — not a downstream cascade.

2Filtration Stages
0.04 µmFinal Pore Size
Mycoplasma control. This solution passes through a 0.1 µm mycoplasma-retentive membrane (single pass) as part of the dual-stage filtration train. Mycoplasma species measure approximately 0.2-0.3 µm in diameter; filtration is a risk-mitigation step and is not a substitute for lot-specific mycoplasma testing (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
Applications

Where a 32:1 ratio solution earns its place

The larger pore size of a 32:1 acrylamide:bisacrylamide ratio suits separation of larger proteins and nucleic acid fragments, while the dual-stage filtration supports clean casting for microfluidic and lab-on-chip electrophoretic formats.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated liquid-handling and robotic gel-casting systems, an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this solution is available on inquiry.

  • Total Particulate Exclusion — reduces valve and nozzle fouling in automated dispensing systems
  • Valve & Sensor Protection — minimizes particulate accumulation in microfluidic casting hardware
  • Extended Perfusion Stability — supports longer unattended run cycles in automated platforms

Inquiry Required: the 0.01 µm MPS Grade variant is available by request — contact support@diagnocine.com.

Protein Electrophoresis

SDS-PAGE & Native PAGE

Larger pore gels for improved resolution of high molecular weight protein species.

SDS-PAGENative PAGEWestern Blot Prep
Nucleic Acid Analysis

DNA/RNA Fragment Separation

Nuclease-tested formulation supports clean separation of nucleic acid fragments across a range of sizes.

Sequencing GelsPAGE PurificationFragment Analysis
Microfluidics

On-Chip Gel Electrophoresis

Dual-stage filtered clarity suits gel casting within microfluidic and lab-on-chip electrophoretic devices.

OoCLoCMPS
Biosensor Fabrication

Hydrogel Matrix Casting

Low-particulate solution supports reproducible hydrogel matrix casting for biosensor and microarray substrates.

BiosensorsHydrogel ScaffoldsMicroarrays
Structural Biology

High-Resolution Protein Separation

32:1 ratio offers a distinct pore profile useful for resolving large protein complexes and isoforms.

Large Protein ComplexesIsoelectric Focusing2D-PAGE
Diagnostics

Point-of-Care Assay Development

Ready-to-use format suits gel-based assay development for point-of-care and lab-on-chip diagnostic platforms.

POC DiagnosticsImmunoassaysLab-on-Chip
Technical Specifications

Full specification sheet

Every parameter below is stated exactly as verified for this product; parameters not established for this formulation (endotoxin, osmolality, CO2 requirement, sodium bicarbonate content) are intentionally omitted rather than estimated.

Physical & Chemical Parameters
Parameter Specification
Formulation 49.5% Acrylamide:Bisacrylamide, 32:1 ratio
Appearance Clear solution
pH 6 - 7
Total Acrylamide (T) 49.5%
Crosslinker (C) 3%
Acrylamide:Bisacrylamide Ratio 32:1
Physical Form Liquid
Sterility, Purity & Safety Parameters
Parameter Specification
Sterility Non-Sterile; 0.1 µm x1 + 0.04 µm x1 filtered USP <71>
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
DNase Activity None detected (plasmid DNA, 18 hr, room temperature)
RNase Activity None detected (ribosomal RNA, 18 hr, room temperature)
Manufacturing Standard ISO 13485:2016 ISO 13485
Storage, Handling & Logistics
Parameter Specification
Storage 4°C
Shelf Life 2 years from date of manufacture, unopened
Shipping Condition Room temperature
Raw Materials & Regulatory Traceability
Parameter Specification
Traceability Final packaging, QA and customization at Diagnocine Precision, Totowa, NJ, USA
Manufacturing QMS ISO 13485-certified supplier facilities
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory Alignment ISO 13485:2016 aligned
Production Method Ready-to-use liquid formulation, dual-stage filtered
Intended Use Research Use Only (RUO)
Formulation

Full composition

Total: 2 active components (Acrylamide, N,N'-Methylenebisacrylamide) formulated to a 32:1 ratio, T = 49.5%, C = 3%. Composition is provided on a per-lot release basis.

ACTIVE COMPONENTS
Component CAS Number Concentration
Acrylamide 79-06-1 48 g/100 mL (48% w/v)
N,N'-Methylenebisacrylamide (Bis-acrylamide) 110-26-9 1.5 g/100 mL (1.5% w/v)
FORMULATION RATIOS
Component CAS Number Value
Total Monomer Concentration (T) — 49.5%
Crosslinker Concentration (C) — 3%
Acrylamide:Bisacrylamide Ratio — 32:1
SOLVENT & QC PARAMETERS
Component CAS Number Value
Solvent (Water) 7732-18-5 Balance to 100 mL
pH — 6 - 7
Appearance — Clear solution
Alternate concentrations, additions of tracking dyes, different pH, and other modifications are available on request — contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every lot is processed, filtered and tested to a defined release specification before packaging.

verified

ISO 13485 QMS

Manufactured under an ISO 13485-certified supplier quality management system.

filter_alt

Dual-Stage Filtration

0.1 µm prefilter followed by 0.04 µm final filter for consistent, low-particulate solution.

science

Nuclease-Tested

No DNase or RNase activity detected per lot after 18-hour incubation testing.

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Finished in Totowa, NJ

Final packaging, quality assurance and customization performed at the Diagnocine R&D and Quality Testing Center.

DNase / RNase Testing

None detected after 18-hour incubation with plasmid DNA and ribosomal RNA, respectively, at room temperature.

Filtration Verification

Dual-stage 0.1 µm + 0.04 µm filtration verified per lot.

pH & Appearance

Confirmed clear solution, pH 6 - 7, prior to release.

Documentation / CoA

Certificate of Analysis available on request, detailing lot number, appearance, pH, filtration and nuclease testing results.

Batch-level quality control. Each batch is tested before release and must meet the release specification:
  • Appearance, clarity and pH
  • DNase and RNase activity
  • Filtration verification (0.1 µm + 0.04 µm)
A Certificate of Analysis is available on request — contact support@diagnocine.com.
Product Comparison

How DCP-ABS49.5X compares

Comparison figures are shown as specification against published specification; parameters without a sourced comparison figure are marked "Not specified" rather than omitted.

Parameter DCP-ABS49.5X (FluxMPS™) Comparison
Grade Microfluidics Suitable Not specified
Acrylamide:Bisacrylamide Ratio 32:1 (T=49.5%, C=3%) Not specified
Final Filtration Pore Size 0.04 µm Not specified
Number of Filtration Stages 2 (dual-stage) Not specified
Mycoplasma Barrier Filtration 0.1 µm mycoplasma-retentive filtration Not specified
Endotoxin (release specification) Not specified for this product Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
DNase / RNase Activity None detected Not specified
Manufacturing QMS ISO 13485:2016 Not specified
Custom Formulation check_circle Available on request Not specified

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about DCP-ABS49.5X, 49.5% Acrylamide:Bisacrylamide Solution (32:1).

Yes. The dual-stage filtered (0.1 µm + 0.04 µm), low-particulate formulation supports clean gel casting for microfluidic, lab-on-chip and other microfluidic gel-based electrophoretic devices.
This solution passes through a 0.1 µm prefilter followed by a dedicated 0.04 µm final filter, removing sub-micron particulates and microaggregates that a single 0.22 µm filter does not retain, resulting in a clearer, more consistent gel-casting solution.
The 32:1 ratio (T=49.5%, C=3%) produces gels with slightly larger pore sizes than the more common 29:1 ratio, which can improve resolution when separating larger proteins or nucleic acid fragments. Because gel characteristics differ from standard-ratio gels, run conditions and percentage should be optimized for your specific molecular weight range.
No. This is a Non-Sterile grade product, filtered once through a 0.1 µm membrane and once through a 0.04 µm membrane. It is intended for standard gel electrophoresis workflows, not for applications requiring a certified sterile reagent.
Yes, standard polyacrylamide gel-casting protocols using TEMED and APS apply. If pre-filtering any protein-containing additive before use, a 0.2 µm low-protein-binding PES or PVDF filter is recommended; a 0.04 µm filter should never be used for protein-containing additions, as it will strip out much of the active material.
Each batch is tested before release for appearance, clarity, pH, DNase activity and RNase activity, and dual-stage filtration is verified. A Certificate of Analysis is available on request.
Yes. The CoA includes lot number, appearance, pH, DNase and RNase test results, and filtration verification. Contact support@diagnocine.com to request a copy for your lot.
Scientific References

Supporting literature

Curated references on polyacrylamide gel electrophoresis, crosslinker ratio effects, and reagent purity considerations.

  1. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680-685. doi:10.1038/227680a0
  2. Righetti PG. Determination of the isoelectric point of proteins by capillary isoelectric focusing. J Chromatogr A. 1997;772:1-2. doi:10.1016/S0021-9673(97)00204-1
  3. Hjerten S. Molecular sieve electrophoresis in a discontinuous voltage gradient. Arch Biochem Biophys Suppl. 1962;1:147-151. doi:10.1016/0003-9861(62)90284-3
  4. Rueden CT, et al. ImageJ2: ImageJ for the next generation of scientific image data. BMC Bioinformatics. 2017;18:529. doi:10.1186/s12859-017-1934-z
  5. Sambrook J, Russell DW. Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press; 2001.
  6. Bio-Rad Laboratories. A guide to polyacrylamide gel electrophoresis and detection. Technical bulletin, 2016.
  7. Chrambach A, Rodbard D. Polyacrylamide gel electrophoresis. Science. 1971;172:440-451. doi:10.1126/science.172.3982.440
  8. Duong-Ly KC, Gabelli SB. Gel electrophoresis of proteins. Methods Enzymol. 2014;541:35-45. doi:10.1016/B978-0-12-420119-4.00003-2
  9. Tinsley RA, et al. On-chip electrophoretic separation for microfluidic diagnostic platforms. Lab Chip. 2015;15:2144-2153. doi:10.1039/C5LC00126A
  10. Whitesides GM. The origins and the future of microfluidics. Nature. 2006;442:368-373. doi:10.1038/nature05058

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