2X PCR Buffer for KOD FX, 5mL (3X 1.67ml)
FluxMPS™ 2X PCR Buffer for KOD FX, 5mL (3X 1.67ml)
FluxMPS™ 2X PCR Buffer for KOD FX is a TOYOBO-manufactured molecular biology reagent supplied by Diagnocine for use with KOD FX DNA polymerase in long-range and GC-rich PCR protocols. This 5 mL format (3 x 1.67 mL) is processed under Diagnocine's ISO 13485:2016-aligned quality framework and filtered through a 0.1 µm membrane to help minimize particulate carryover into sensitive amplification workflows. As a special-order product, please inquire with our technical support team before purchasing to confirm current availability, pricing, and lot-specific specifications.
- Single-stage 0.1 µm membrane filtration prior to fill
- Formulated for KOD FX DNA polymerase-based long-range and GC-rich PCR
- Manufactured by TOYOBO CO., LTD.; distributed by Diagnocine under an ISO 13485:2016-aligned quality system
- Supplied in a 5 mL format (3 x 1.67 mL) for flexible aliquoting
- Cold-chain shipped on dry ice; stored at -20°C to preserve reagent integrity
- Special-order, inquire-only product — contact support@diagnocine.com before purchasing
- For Research Use Only (RUO)
- Custom packaging and formulation available on request
- Cat. No.TYB-KFX-2B
- Packaging5 mL (3 x 1.67 mL)
- Storage Temperature-20°C
- Shipping ConditionShips on Dry Ice
- BrandTOYOBO
- ManufacturerTOYOBO CO., LTD.
- UNSPSC CategoryIndicators and reagents
- Order TypeSpecial Order – Inquire Before Purchasing
- Manufacturing StandardISO 13485:2016-aligned
- Intended UseResearch Use Only (RUO)
Engineered for reliable molecular biology results
Standard, unspecified PCR reagents can introduce particulate carryover, inconsistent sourcing, and untracked lot handling into sensitive amplification workflows. FluxMPS™ positions this TOYOBO-manufactured buffer within a documented, traceable, ISO 13485:2016-aligned distribution framework.
Amplicon-Safe Purity
Single-stage 0.1 µm membrane filtration helps reduce particulate that can interfere with downstream PCR and molecular biology workflows.
Formulated for KOD FX Performance
Buffered specifically to support KOD FX DNA polymerase in long-range and GC-rich amplification protocols.
TOYOBO Manufacturing Heritage
Sourced directly from TOYOBO CO., LTD., the original manufacturer of KOD FX polymerase chemistries.
Low-Background Reagent Handling
Supports clean amplification for downstream sequencing, genotyping, and imaging-based verification workflows.
Defined, Traceable Sourcing
Catalog number, packaging, and storage conditions are documented and traceable through Diagnocine's ISO 13485:2016-aligned distribution system.
Customization & Special-Order Support
Packaging and formulation adjustments may be available on request — contact support@diagnocine.com before purchasing.
Single-stage 0.1 µm filtration
This PCR buffer is processed through a single 0.1 µm membrane filtration stage prior to fill, supporting reduced particulate levels for downstream molecular biology workflows.
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0.1 µm Pre-filtration
Removes larger particulates and aggregates prior to fill, supporting cleaner reagent handling for PCR and downstream molecular biology assays.
Performance vs. conventional buffer
0.1 µm membrane filtration provides finer particulate control than reagents filtered only to a standard 0.22 µm pass.
© Diagnocine® — TYB-KFX-2B
Where this PCR buffer supports your workflow
Formulated for KOD FX DNA polymerase, this buffer supports long-range and GC-rich amplification used across molecular biology, genotyping, and quality-control workflows in life-science research.
Automated Bioreactors & Robotics
For laboratories integrating this PCR buffer into automated liquid-handling or robotic PCR setup platforms, Diagnocine offers custom packaging and formulation configurations on a special-order basis to support high-throughput and automated workflows.
- Custom aliquot formats for automated liquid handlers
- Special-order packaging configurations available on request
- Dedicated technical support for integration into automated PCR workflows
Inquiry Required: As a special-order product, please contact support@diagnocine.com to discuss automation-compatible configurations before purchasing.
Micro Physiological System (MPS) & Chip
Supports PCR-based genetic verification and quality-control workflows for microfluidic and organ-on-a-chip model development.
Reconstitution of Amplification Reactions
Used in preparing PCR reaction mixes for downstream molecular biology sample-processing workflows.
iPSC-Derived Model Handling
Supports PCR-based identity and genotyping verification of iPSC-derived lines used in downstream research models.
Endothelial & Primary Cell Perfusion
Supports PCR-based screening and identity confirmation of primary endothelial cultures used in perfusion-based studies.
ELISA, Blotting & Blocking Support
Supports PCR-based confirmation of target sequences preceding antibody validation and immunoassay workflows.
Microscopy & Optical Sensing
Supports PCR-based genotyping and verification of reporter lines used in live-cell imaging and biosensor assays.
Confirmed product parameters
Only parameters confirmed for this product are listed below; additional lot-specific specifications are available upon inquiry.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Proprietary 2X PCR buffer formulated for KOD FX DNA polymerase; detailed composition provided upon inquiry |
| Packaging Format | 5 mL (3 x 1.67 mL) |
| Parameter | Specification |
|---|---|
| Manufacturing Standard ISO 13485 | ISO 13485:2016-aligned quality system |
| Filtration | Single-stage 0.1 µm membrane filtration |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shipping Condition | Ships on Dry Ice |
| Order Type | Special Order – Inquire Before Purchasing |
| Parameter | Specification |
|---|---|
| Manufacturer | TOYOBO CO., LTD. |
| Brand | TOYOBO |
| Regulatory Alignment ISO 13485 | ISO 13485:2016-aligned distribution |
| Intended Use | Research Use Only (RUO) |
Full composition
This is a specialty, special-order PCR buffer. Full component-level formulation data is confirmed directly with Diagnocine technical support at the time of inquiry.
| Component | CAS Number | Concentration |
|---|---|---|
| Proprietary 2X PCR buffer formulation for KOD FX DNA polymerase | Provided upon inquiry |
Manufacturing & compliance
This TOYOBO-manufactured PCR buffer is distributed by Diagnocine within an ISO 13485:2016-aligned quality framework.
ISO 13485:2016 Quality System
Diagnocine distributes this TOYOBO-manufactured reagent under an ISO 13485:2016-aligned quality management framework.
Ultrapure Type 1 Water Standard
Diagnocine references Ultrapure Type 1 water (18.2 MΩ·cm) as a foundational benchmark across its reagent catalog; lot-specific documentation is available upon inquiry.
ISO Class 5 Fill & Finish Standard
Diagnocine's reference fill environment standard is ISO Class 5 (Class 100); confirmation for this specific lot is available upon inquiry.
Micro-Batch Precision & Special-Order Handling
As a special-order, inquire-only product, this PCR buffer is documented individually at time of quote.
Endotoxin (USP <85>)
Bacterial Endotoxins Test methodology per USP <85>; product-specific results for this lot are available upon inquiry.
Particulate (USP <788>)
Particulate control referenced against USP <788> Method 2; lot-specific data available upon inquiry.
Osmolality (USP <785>)
Osmolality methodology referenced against USP <785>; product-specific value for this lot is available upon inquiry.
Documentation / CoA
A Certificate of Analysis for this lot is available upon request.
How TYB-KFX-2B compares
A qualitative comparison based on sourcing, traceability, and distribution practices for this special-order PCR buffer.
| Parameter | TYB-KFX-2B (FluxMPS™) | Conventional PCR Buffer | Standard Alternative |
|---|---|---|---|
| Direct manufacturer traceability (TOYOBO) | check_circle | cancel | cancel |
| 0.1 µm membrane filtration | check_circle | cancel | cancel |
| Special-order custom packaging | check_circle | cancel | cancel |
| ISO 13485:2016-aligned distribution | check_circle | cancel | check_circle |
| Certificate of Analysis on request | check_circle | cancel | check_circle |
Frequently asked questions
Answers reflect this product's special-order, inquire-only nature.
Supporting literature
General reference literature relevant to PCR buffer chemistry, KOD FX polymerase applications, and molecular biology quality control in microfluidic and organ-on-a-chip research.
- Ishino Y, et al. Long and accurate PCR with a mixture of KOD DNA polymerase and its exonuclease-deficient mutant enzyme. doi:10.1006/jbbm.1998.0002
- Nishioka M, et al. Long and accurate PCR with a mixture of KOD DNA polymerase and its exonuclease-deficient mutant enzyme II. doi:10.1016/S0168-1656(01)00294-8
- Bhadra S, et al. Real-time sequence-validated loop-mediated isothermal amplification assays for detection of Middle East respiratory syndrome coronavirus. doi:10.1371/journal.pone.0123126
- Huggett JF, et al. The digital MIQE guidelines. doi:10.1373/clinchem.2013.206375
- Bustin SA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. doi:10.1373/clinchem.2008.112797
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. doi:10.1038/nbt.2989
- Freshney RI. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, quality-control genotyping considerations. doi:10.1002/9781118873656
- Almeida JL, et al. Standards for cell line authentication and beyond. doi:10.1371/journal.pbio.2001177
