2X PCR Buffer for KOD FX, 100ml
FluxMPS™ 2X PCR Buffer for KOD FX, 100 mL
A specialized 2X PCR buffer formulated for use with KOD FX high-fidelity DNA polymerase, originally manufactured by TOYOBO CO., LTD. and distributed by Diagnocine under FluxMPS™ quality-controlled handling. Supplied at 100 mL, stored at -20°C, and shipped on dry ice to preserve reagent integrity for molecular biology, genomics, and microfluidic PCR workflows. This is a special-order, inquiry-based product — please contact support@diagnocine.com before purchasing to confirm current availability and configuration.
- Specialized 2X PCR buffer formulated to support KOD FX DNA polymerase-based amplification chemistry
- Supplied in a 100 mL format, stored at -20°C and shipped on dry ice to preserve reagent integrity
- Distributed by Diagnocine under ISO 13485:2016-aligned quality management
- Handled with ultrapure Type 1 water (18.2 MΩ·cm) quality standards throughout the FluxMPS™ supply chain
- Special-order reagent — contact support@diagnocine.com prior to purchase to confirm current configuration
- Supports high-fidelity, GC-rich, and long-range PCR protocols requiring KOD FX polymerase chemistry
- Suitable for molecular biology, genomics, and microfluidic lab-on-a-chip (LoC) PCR workflows
- Custom packaging, documentation, and configuration available upon request
- Catalog No.TYB-KFX-1B1
- ManufacturerTOYOBO CO., LTD.
- Buffer Type2X Concentrate
- Packaging100 mL
- Storage Temp.-20°C
- Shipping ConditionShips on Dry Ice
- UNSPSC CategoryBuffers (12161700)
- Product ClassSpecialized Reagent
- Intended UseResearch Use Only (RUO)
- AvailabilitySpecial Order — Inquire Before Purchase
Engineered where standard buffers fail
Conventional, unmanaged PCR reagents can carry subvisible particulates, inconsistent lot-to-lot handling, and undocumented cold-chain history — all of which can compromise sensitive amplification chemistry. FluxMPS™ distribution standards are built to remove that uncertainty.
Molecular-Grade Purity
Manufactured with a controlled 0.1 µm membrane filtration step to reduce gross particulate prior to packaging.
Chemistry Matched to KOD FX
Formulated specifically to support KOD FX DNA polymerase amplification of GC-rich and long-range templates.
Ultrapure-Grade Water
Handled to Type 1, 18.2 MΩ·cm water quality standards consistent with USP <85> expectations.
Low Background for Downstream Analysis
Designed to minimize interference in qPCR, dPCR, and NGS library preparation detection chemistries.
Manufacturer-Controlled Formulation
Originally formulated and lot-released by TOYOBO CO., LTD. and distributed under a controlled chain of custody.
Customization on Demand
Packaging size, documentation, and configuration are available on request — contact support@diagnocine.com.
Single-stage 0.1 µm filtration
This reagent passes through a controlled 0.1 µm membrane filtration step during manufacturing, reducing gross particulate content prior to packaging and cold-chain shipment.
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1
0.1 µm Pre-Filtration
Removes larger particulate and aggregate material as part of the standard manufacturing process.
Manufacturing quality control
A controlled 0.1 µm filtration step supports reduced particulate content relative to unfiltered or undocumented buffer sources.
© Diagnocine® — TYB-KFX-1B1
Where TYB-KFX-1B1 is used
As a KOD FX-optimized 2X PCR buffer, this reagent supports amplification steps across molecular biology, genomics, and microfluidic PCR workflows.
Automated Bioreactors & Robotics
For automated liquid handling and robotic PCR setup platforms, a further ultra-filtered variant may be available to help protect valves and sensors from particulate accumulation.
- Total Particulate Exclusion for automated fluidic pathways
- Valve & Sensor Protection in robotic liquid handlers
- Extended Reagent Line Stability during high-throughput runs
Inquiry Required: contact support@diagnocine.com to discuss availability of an ultra-filtered variant for automated platforms.
Microfluidic & On-Chip PCR Amplification
Supports amplification steps within microfluidic and lab-on-a-chip PCR platforms.
PCR Master Mix Assembly & Reaction Setup
Used as the base buffer component for master mix assembly ahead of amplification.
High-Fidelity & GC-Rich Template Amplification
Formulated to support KOD FX chemistry across difficult, GC-rich, and long-range templates.
Research-Use Genotyping & Detection Assays
Applicable to research-stage genotyping and pathogen-detection assay development.
Quantitative & Digital PCR Workflows
Supports amplification chemistries feeding quantitative and digital PCR platforms and on-chip biosensors.
Robotic & Automated PCR Setup
Compatible with automated liquid handling platforms for high-throughput PCR reaction assembly.
Documented parameters
The parameters below reflect only the information published by the manufacturer for this specialized reagent. Detailed formulation and performance data can be requested via support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Buffer Type | 2X Concentrate |
| Formulation | Proprietary 2X PCR Buffer optimized for KOD FX DNA Polymerase |
| Packaging Volume | 100 mL |
| Parameter | Specification |
|---|---|
| Water Purity USP <85> | Ultrapure Type 1 (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485:2016-aligned |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shipping Condition | Ships on Dry Ice |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO 13485 | ISO 13485:2016 |
| Intended Use | Research Use Only (RUO) |
| Original Manufacturer | TOYOBO CO., LTD. |
Full composition
Detailed component-level formulation for this proprietary 2X PCR buffer is not publicly disclosed by the manufacturer. The concentration factor stated by the manufacturer is provided below.
| Component | CAS Number | Concentration |
|---|---|---|
| 2X PCR Buffer System (proprietary formulation for KOD FX) | — | 2X concentrate |
Manufacturing & compliance
Diagnocine distributes this reagent under a controlled quality framework, while formulation-specific documentation originates from the manufacturer, TOYOBO CO., LTD.
ISO 13485:2016 QMS
Distributed under an ISO 13485:2016-aligned quality management system.
Ultrapure Type 1 Water
Handled to Type 1, 18.2 MΩ·cm water quality standards.
Controlled Cold-Chain Handling
Stored at -20°C and shipped on dry ice to preserve reagent integrity.
Special-Order Traceability
Configuration confirmed with the manufacturer prior to fulfillment for each order.
Particulate Control USP <788>
Manufactured with reference to USP <788>-type particulate control frameworks where applicable.
Sterility Testing USP <85>
Endotoxin and sterility documentation, where available, follows USP <85>-referenced methodology.
Quality Attributes as Applicable
Formulation-specific parameters are assessed only where applicable to this reagent's intended use.
Documentation / CoA
A Certificate of Analysis can be requested via support@diagnocine.com, subject to manufacturer availability.
How TYB-KFX-1B1 compares
A comparison of documented handling and quality attributes against typical, undocumented PCR buffer sourcing.
| Parameter | TYB-KFX-1B1 (FluxMPS™) | Standard PCR Buffer (Unspecified Grade) | Generic Lab-Prepared Buffer |
|---|---|---|---|
| Manufacturer-Optimized for KOD FX Chemistry | check_circle | cancel | cancel |
| Manufacturing QMS (ISO 13485:2016) | check_circle | cancel | cancel |
| Ultrapure Type 1 Water (18.2 MΩ·cm) | check_circle | cancel | cancel |
| Cold-Chain Shipping (Dry Ice, -20°C Storage) | check_circle | cancel | cancel |
| Documented Certificate of Analysis | check_circle | cancel | cancel |
| Custom Packaging & Formulation on Request | check_circle | cancel | cancel |
| Compatible with Microfluidic PCR Workflows | check_circle | cancel | cancel |
Frequently asked questions
Answers below reflect only information published by the manufacturer for this specialized, special-order reagent.
Supporting literature
Product-specific citations were not identified for TYB-KFX-1B1. The curated literature below provides background on high-fidelity PCR chemistry and microfluidic PCR applications relevant to this reagent's use.
- Nagai M, et al. "Development of a novel long and accurate PCR method using a mixture of DNA polymerases." doi:10.1006/bbrc.1998.9490
- Barnes WM. "PCR amplification of up to 35-kb DNA with high fidelity and high yield from lambda bacteriophage templates." doi:10.1073/pnas.91.6.2216
- Zhang Y, et al. "Microfluidic PCR chips for rapid nucleic acid amplification." doi:10.1039/C6LC01035K
- Zhu Q, et al. "Digital PCR on a microfluidic chip for absolute nucleic acid quantification." doi:10.1021/ac503688g
- Kim TH, et al. "Lab-on-a-chip technologies for molecular diagnostics." doi:10.1038/s41551-017-0058-1
- Higuchi R, et al. "Kinetic PCR analysis: real-time monitoring of DNA amplification reactions." doi:10.1038/nbt0993-1026
- Whitesides GM. "The origins and the future of microfluidics." doi:10.1038/nature05058
- Zhang C, Xing D. "Miniaturized PCR chips for nucleic acid amplification and analysis." doi:10.1093/nar/gkm121
