0.625% Glutaraldehyde in 0.1 M Phosphate Buffer
FluxMPS™ 0.625% Glutaraldehyde in 0.1 M Phosphate Buffer
An MPS-grade, quadruple-stage filtered fixative buffer combining 0.625% glutaraldehyde with a 0.1 M phosphate buffering system held at pH 7.4. Formulated for high-precision electron microscopy, immunohistochemistry, and perfusion fixation workflows where ultrastructural detail and antigenicity must both be preserved. Filtered 0.1 µm membrane twice and 0.04 µm membrane twice for mycoplasma-safe, ultra-clean fixation chemistry.
- Quadruple-stage filtration: 0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice
- Precise, stable pH 7.4 in a 0.1 M phosphate buffer system
- 0.625% glutaraldehyde concentration for deep tissue penetration with reduced over-fixation artifacts
- Ultrapure Type 1 water base (18.2 MΩ·cm)
- DNase and RNase activity: none detected
- Compatible with TEM/SEM, immunogold labeling, and perfusion fixation protocols
- Manufactured under ISO 13485-certified, CE-approved facilities
- Customizable concentration, pH, and formulation available on request
- pH7.4
- Glutaraldehyde0.625%
- Phosphate Buffer Molarity0.1 M
- Filtration0.1 µm x2 + 0.04 µm x2
- AppearanceClear solution
- DNase ActivityNone detected
- RNase ActivityNone detected
- Storage (Short-Term)4°C, protected from light
- Storage (Long-Term)-20°C, protected from light
- Shelf Life6 months (4°C) / 18 months (-20°C, unopened)
Engineered where standard fixative buffers fail
Conventional 0.22 µm-filtered fixative solutions can carry subvisible particulates, uncontrolled pH drift, and inconsistent aldehyde crosslinking that compromise ultrastructural detail. FluxMPS™ 0.625% Glutaraldehyde in 0.1 M Phosphate Buffer is built to remove those failure modes at the source.
Mycoplasma-safe purity
Quadruple-stage 0.1 µm and 0.04 µm membrane filtration, each applied twice, removes fine particulates and bioburden before fill.
Precise, stable pH 7.4
A defined 0.1 M phosphate buffer system stabilizes pH throughout fixation, critical for preserving ultrastructural detail and preventing acid hydrolysis.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), consistent with USP <85> water-quality practice.
Low-artifact ultrastructural fixation
A moderate 0.625% glutaraldehyde concentration allows deeper tissue penetration than higher concentrations, reducing over-fixation while retaining antigenicity for downstream imaging.
Defined, traceable composition
Every lot is formulated to 0.625% glutaraldehyde in 0.1 M phosphate buffer with documented DNase- and RNase-free status.
Customization on demand
Alternate glutaraldehyde concentrations, pH values, and chemical/protein additions are available — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of DCP-GAP0.6X is filtered through a 0.1 µm membrane twice and a 0.04 µm membrane twice, giving this fixative buffer a sub-0.1 µm final polish appropriate for mycoplasma-safe, ultra-clean sample preparation.
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1
0.1 µm Pre-filtration I
Removes large particulates and aggregates, extending the life of downstream filters.
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2
0.04 µm Pre-filtration II
Retains fine particulates and bioburden ahead of the second membrane pass.
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3
0.1 µm Sterile-filtration I
A second 0.1 µm pass provides redundant particulate removal before final polish.
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4
0.04 µm Sterile-filtration II — Final Polish
The second 0.04 µm pass delivers the final polish; because the smallest mycoplasma type is approximately 0.2 microns, this pore size prevents mycoplasma contamination in the finished buffer.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, each applied twice, removes finer particulates than a single 0.22 µm pass used in conventional fixative buffers, supporting mycoplasma-safe, ultra-clean fixation chemistry for ultrastructural studies.
© Diagnocine® — DCP-GAP0.6X
Where DCP-GAP0.6X is used
A pre-mixed glutaraldehyde/phosphate fixative built for structural studies that require both crosslinking efficacy and pH stability.
Automated Bioreactors & Robotics
For automated perfusion-fixation platforms and robotic liquid handling in electron-microscopy sample-prep pipelines, an optional 0.01 µm (10 nm) ultra-filtered variant of this fixative buffer is available on request.
- Total Particulate Exclusion
- Valve & Sensor Protection
- Extended Perfusion Stability
Inquiry Required: the 0.01 µm ultra-filtered grade is produced to order; contact support@diagnocine.com to request it.
TEM & SEM Sample Fixation
The primary application of this fixative is transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Its 0.625% glutaraldehyde concentration allows deeper tissue penetration than 2.5–5% formulations, reducing over-fixation artifacts while stabilizing organelles, membranes, and cytoskeletal structures.
Immersion & Perfusion Fixation
Used for immersion fixation of small biopsies (≤1 mm3) and perfusion fixation of organs, preserving tissue architecture for light microscopy, immunohistochemistry, and long-term storage.
Enzyme Histochemistry & Immunogold Labeling
Compatible with post-fixation processing for enzyme histochemistry and immunogold labeling, with moderate crosslinking density that retains antigenicity better than higher glutaraldehyde concentrations.
Potassium-Sensitive Antibody Compatibility
In variants without potassium in the phosphate buffer, interference with potassium-sensitive antibodies is avoided, making this formulation suitable for neurological and immunological studies.
Organ-on-a-Chip Tissue Fixation
Applicable to fixation of on-chip tissue and organoid models within microphysiological system (MPS), organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and body-on-a-chip (BoC) workflows.
Post-Fixation Imaging & Long-Term Storage
Preserved tissue architecture supports light microscopy and immunohistochemistry workflows as well as long-term archival storage of fixed specimens.
Full specification sheet
Values below are as declared for DCP-GAP0.6X.
| Parameter | Specification |
|---|---|
| Formulation | 0.625% Glutaraldehyde in 0.1 M Phosphate Buffer |
| Appearance | Clear solution |
| pH USP <791> | 7.4 |
| Glutaraldehyde Concentration | 0.625% |
| Phosphate Buffer Molarity | 0.1 M |
| Parameter | Specification |
|---|---|
| Sterility (Filtration) | 0.1 µm membrane twice, 0.04 µm membrane twice |
| DNase Activity | None detected (plasmid DNA, 18 hr, room temperature) |
| RNase Activity | None detected (ribosomal RNA, 18 hr, room temperature) |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facility |
| Parameter | Specification |
|---|---|
| Storage (Short-Term) | 4°C, away from bright light (amber) |
| Storage (Long-Term) | -20°C, away from bright light |
| Shelf Life (4°C) | 6 months |
| Shelf Life (-20°C, unopened) | 18 months |
| Shipping Condition | Ships typically within 48 hours; may take up to 1 week during unforeseen events |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified, CE-approved facility |
| Final Packaging & QA | DiagnoCine R&D and Quality Testing Center |
| Custom Assembly Location | DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
DCP-GAP0.6X combines a defined glutaraldehyde concentration with a 0.1 M phosphate buffering system for consistent, lot-released fixation chemistry.
| Component | CAS Number | Concentration |
|---|---|---|
| Glutaraldehyde | 111-30-8 | 0.625% |
| Phosphate Buffer | 0.1 M |
Manufacturing & compliance
DCP-GAP0.6X is manufactured under ISO 13485-certified, CE-approved facilities, with final packaging, quality assurance, and testing completed at the DiagnoCine R&D and Quality Testing Center.
ISO 13485:2016 QMS
Manufactured under an ISO 13485-certified, CE-approved quality management system.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm).
ISO Class 5 Fill & Finish
Final fill performed under controlled, aseptic conditions.
Micro-Batch Precision
Custom assembly and packaging completed at DiagnoCine Precision, Totowa, New Jersey, USA.
DNase Activity
None detected after incubation of plasmid DNA with this product for 18 hours at room temperature.
RNase Activity
No RNase activity detected after incubation of ribosomal RNA with this product for 18 hours at room temperature.
Sterility — Quadruple-Stage Filtration
Filter-sterilized with 0.1 µm filtration twice and 0.04 µm filtration twice, preventing mycoplasma contamination.
Documentation
A Certificate of Analysis (CoA) is available for this lot — contact support@diagnocine.com.
How DCP-GAP0.6X compares
A side-by-side look at filtration architecture and formulation control versus conventional fixative buffers.
| Parameter | DCP-GAP0.6X (FluxMPS™) | Conventional 0.22 µm-Filtered Buffer | Standard Alternative |
|---|---|---|---|
| Defined glutaraldehyde/phosphate formulation | check_circle | cancel | cancel |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| DNase-free verified | check_circle | cancel | cancel |
| RNase-free verified | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Standard purified water | Standard purified water |
| Manufacturing QMS | ISO 13485-certified | Unspecified | Unspecified |
| Compatible with TEM/SEM ultrastructural studies | check_circle | check_circle | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-GAP0.6X, 0.625% Glutaraldehyde in 0.1 M Phosphate Buffer.
Supporting literature
Curated literature relevant to glutaraldehyde-phosphate fixation and microfluidic tissue modeling.
- Sabatini DD, Bensch K, Barrnett RJ. Cytochemistry and electron microscopy: the preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963. doi:10.1083/jcb.17.1.19
- Hopwood D. Theoretical and practical aspects of glutaraldehyde fixation. Histochem J. 1972. doi:10.1007/BF01003466
- Hayat MA. Principles and Techniques of Electron Microscopy: Biological Applications. Cambridge University Press. doi:10.1017/CBO9780511810128
- Kiernan JA. Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: what they are and what they do. Microsc Today. 2000. doi:10.1017/S1551929500057060
- Ushiki T. Collagen fibers, reticular fibers and elastic fibers: a comprehensive understanding from a morphological viewpoint. Arch Histol Cytol. 2002. doi:10.1679/aohc.65.109
- Bullock GR. The current status of fixation for electron microscopy: a review. J Microsc. 1984. doi:10.1111/j.1365-2818.1984.tb00458.x
- Huebinger J, et al. Direct measurement of glutaraldehyde and formaldehyde crosslinking kinetics in tissue. Sci Rep. 2016. doi:10.1038/srep29305
- Ingber DE. Reverse engineering human pathophysiology with organs-on-chips. Cell. 2016. doi:10.1016/j.cell.2016.05.048
- Zucker RM, Daniel KM. Detection of TiO2 nanoparticles in cells by flow cytometry and light-scattering techniques, relevant to fixation and buffer purity control. Methods Mol Biol. 2012. doi:10.1007/978-1-61779-953-2_23
- Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev. 1998. doi:10.1128/MMBR.62.4.1094-1156.1998
