Real-time PCR
Real-time PCR Kits
DiagnoCine offers TOYOBO® Taq DNA Polymerases in the form of Master Mix (Probe), SYBR Green Master Mix (Plus), Thunderbird qPCR (Probe), and Thunderbird SYBR qPCR formats. All Taq DNA Polymerases will provide the highest specificity by Hot Start technology, excellent Passive reference, and fits well for glass capillaries for many purposes.
• Real-time PCR Master Mix is a probe assay type with the highest hot start specificity, fast routine PCR experiments, and works well for GC-rich regions. This is for routine short-length PCR targets and not suitable for the long target (>10kb length; KOD is best for long-range size PCR) PCR. It can use for crude samples but KOD is best for crude sample amplifications. This product's strongest feature is the high specificity and Probe master mix for routine end-point PCR experiments.
• SYBR Green Realtime PCR Master Mix (plus for a hot start) is not as specific as the other Taq polymerases (i.e. Probe type or Thunderbird SYBR are more specific) master mix but this brand provides an excellent passive reference and fits for glass capillary use. It is designed to work well for routine short-range PCR and light GC-rich regions and fairly clean crude samples.
• Thunderbird Probe qPCR Mix is a probe assay type with hot start specificity, good passive reference, and glass capillary type PCR experiments. The strength is the highest specificity with low noise bands for difficult PCR and routine experiments such as genotyping and colony screening. Promised for best specificity and fast PCR speed but not made for a long-range PCR (>10kb length; KOD is best for long amplicons more than 10kb). It can unwind the GC-rich region and crude samples can be used (KOD is the best for crude sample use). This type is best for all routine laboratory PCR with short-mid range amplicons, looking for fast PCR time but with the strongest specificity and not dealing with PCR noise bands.
• Thunderbird SYBR qPCR Mix is an SYBR assay type with a strong specificity but slightly less specific than Probe qPCR. It has passive reference and glass capillary compatibility. The product is designed for a routine PCR with short to mid-range amplicons for a short and fast PCR time. The best performance is expected with partially clean crude samples and a fair level of GC-rich complexity.
Diagnocine has various real-time PCR reagents showing below with TOYOBO®. The following table shows the characteristics of each product. The detailed information can be referred in the linked sites.
| Enzyme | Product Name | Assay type |
One-step RT-PCR | Hot start(Anti-body) | Passive reference | Glass capillary | Specificity | Efficiency | Long target amplification | GC-rich targets | Amplification from crude samples |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Taq DNA polymerase | Realtime PCR Master Mix | Probe | ✓ | ✓ | ✓ | +++ | ++ | + | + | + | |
| SYBR® Green Realtime PCR Master Mix (-Plus-) | SYBR® | ✓ | ✓ | ✓ | + | ++ | + | + | + | ||
| TUNDERBIRD™ Probe qPCR Mix | Probe | ✓ | ✓ | ✓ | +++ | ++ | + | + | + | ||
| TUNDERBIRD™ SYBR® qPCR Mix | SYBR® | ✓ | ✓ | ✓ | ++ | ++ | + | + | + | ||
| Tth DNA polymerase [One Step] | RNA-direct™ Realtime PCR Master Mix | Probe | ✓ | ✓ | ✓ | ✓ | +++ | ++ | + | ++ | ++ |
| RNA-direct™ SYBR® Green Realtime PCR Master Mix | SYBR® | ✓ | ✓ | ✓ | ✓ | ++ | ++ | + | ++ | ++ | |
| THUNDERBIRD™ Probe One-step qRT-PCR Kit | Prode | ✓ | ✓ | ✓ | ✓ | +++ | +++ | + | ++ | ++ | |
| KOD exo(-) DNA polymerase | KOD SYBR® qPCR Mix | SYBR® | ✓ | ✓ | ✓ | ++ | ++ | +++ (<2 kb) |
+++ | +++ | |
| GenNext™ NGS Library Quantification Kit | SYBR® | ✓ | ✓ | ++ | ++ | +++ (<2 kb) |
+++ | +++ |
+++: BEST, ++: Excellent, +: Good, ✓: Applicable
Realtime PCR Master Mix Efficient qPCR Master Mix for Probe Assay
- Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible probe assay.
- Can be used in glass capillary systems (e.g. LightCycler, Roche Molecular Systems, Inc.).
- Can be used in a passive reference system (e.g., ABI PRISM™7700, Applied Biosystems, Inc.).
This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer and probe. This reagent is applicable in TaqMan™ assays or hybridization probe assays, in combination with each probe.
- Code No. QPK-101 1 mL x 5 200 reactions
SYBR® Green Realtime PCR Master Mix Efficient qPCR Master Mix for Intercalation Assay
- Hot Start technology with anti-Taq DNA polymerase antibodies enables high specificity and reproducible intercalation assay.
- Can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.).
- Can be used in a passive reference system (e.g., ABI PRISM™ 7700, Applied Biosystems, Inc.).
This product is a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for intercalation assay with SYBR® Green I.
- Code No. QPK-201 1 mL x 5 200 reactions
SYBR® Green Realtime PCR Master Mix -Plus- Improved qPCR Master Mix for Intercalation Assay
- Hot Start technology with anti-Taq DNA polymerase antibodies and additives enable excellent specificity and reproducible intercalation assay.
- Can be used in glass capillary systems (e.g., LightCycler, Roche Molecular Systems, Inc.).
- This reagent can be used in a passive reference system (e.g., ABI PRISM™ 7700, Applied Biosystems, Inc.).
This product is an improved version of a Taq DNA polymerase-based 2 x master mix for real-time PCR, which contains all components, except for the primer. This reagent is applicable for highly specific intercalation assay with SYBR® Green I. This reagent is effective for reducing non-specific amplifications or primer dimers.
- Code No. QPK-212 1 mL x 5 200 reactions
THUNDERBIRD™ qPCR Mix High efficient real-time PCR Master Mix
- The specificity for the detection of low-copy targets is improved.
- The dispersion of PCR efficiency between targets is reduced by a new PCR enhancer*. (*Patent pending).
- High specificity and effective amplification enable the detection of a broad dynamic range.
- The master mix contains anti-Taq DNA polymerase antibodies for hot start technology. The antibodies are easily inactivated in the first denaturation step, thereby activating the DNA polymerase.
THUNDERBIRD™ Probe and SYBR® qPCR Mix is a highly efficient 2x Master Mix for real-time PCR using TaqMan™ probes and SYBR® Green I. The master mix contains all required components, except for ROX reference dye, probe and primers (50x ROX reference dye is individually supplied with this kit). The master mix facilitates reaction setup, and improves the reproducibility of experiments.
These products are improved versions of Realtime PCR Master Mix (Code No. QPK-101) and SYBR® Green Realtime PCR Master Mix (Code No. QPK-201) .
- Code No. QPS-101 200 reactions (TaqMan™ probe version)
- Code No. QPS-201 200 reactions (SYBR® Green version)
RNA-direct™ Realtime PCR Master Mix One-step qPCR Master Mix for Probe Assay
- Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
- Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
- Can be used in systems using passive reference (e.g., ABI PRISM™ 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
- Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible probe assay.
This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for "1-step real-time PCR", including reverse transcription and PCR steps. This reagent is applicable for TaqMan™ assay or hybridization probe assay, in combination with each probe.
- Code No. QRT-101 0.5 mL x 5 100 reactions
RNA-direct™ SYBR® Green Realtime PCR Master Mix
One-step qPCR Master Mix for Intercalation Assay
- Suitable for high-throughput real-time PCR and increases reliability of product, due to lowered risk of contamination.
- Can be used in systems using glass capillaries (e.g., LightCycler, Roche Molecular Systems, Inc.).
- Can be used in systems using passive reference (e.g., ABI PRISM™ 7700, Applied Biosystems, Inc.). The passive reference dye does not affect any other systems.
- Hot Start technology, using anti-Tth DNA polymerase antibodies, allows for high specificity and reproducible intercalation assay.
This product is a 2 × Master Mix for "1-step real-time PCR" using a thermostable DNA polymerase derived from Thermus thermophilus (Tth) HB8). Tth DNA polymerase exhibits reverse transcriptase activity in the presence of Mn2+ ions. This system allows for "1-step real-time PCR", including reverse transcription and PCR steps. This reagent can be applied to an intercalation assay with SYBR® Green I.
- Code No. QRT-201 0.5 mL x 5 100 reactions
THUNDERBIRD™ Probe One-step qRT-PCR Kit High Efficient One-step qRT-PCR Kit
- Rapid and highly sensitive
- Tolerant of target sequence diversity
- Tolerant of PCR inhibitors
- Utilization of dUTP
- Multiplex detection
THUNDERBIRD™ Probe One-step qRT-PCR Kit is a one-step real-time reverse-transcription polymerase chain reaction (RT-PCR) kit using the highly efficient reverse transcriptase "ReverTra Ace™" and Tth DNA polymerase as a PCR enzyme. This product can be used mainly in TaqMan™ probe assays. The one-step system is suitable for high-throughput analysis because of its simple reaction setup. In addition, this system can reduce the risk of cross-contamination.
- Code No. QRZ-101 100 reactions
KOD SYBR® qPCR Mix High efficient real-time PCR Master Mix with KOD
- Quantitative analysis can be achieved using long targets, up to 2kb.
- Because of using KOD DNA polymerase, the product is tolerant of PCR inhibitors in crude samples.
- Enable the effective amplification from GC-rich targets.
KOD SYBR® qPCR Mix is a highly efficient 2× Master Mix for real-time PCR using SYBR® Green I and based on the 3’-5’ exonuclease deficient KOD DNA Polymerase [KOD exo(-)]. The master mix contains all the required components, except the ROX reference dye and primers (50x ROX reference dye is supplied separately with this kit). The master mix aids reaction setup, and improves the reproducibility of experiments.
- Code No. QKD-201 200 reactions
GenNext™ NGS Library Quantification Kit Library quantification kit for illumina
- Accurate quantification
KOD SYBR® qPCR Mix can efficiently amplify GC- and AT-rich fragments of different lengths without bias. - Broad dynamic range
The kit has a broad dynamic range from 20 pM (Standard DNA 1) to 0.0002 pM (Standard DNA 6). - Convenient
The kit contains all reagents (KOD SYBR® qPCR Mix, 5x Primer Mix, Standard DNA, and 50x Dilution buffer) needed for the qPCR-based quantification of an NGS library.
GenNext™ NGS Library Quantification Kit is for the SYBR® Green I qPCR-based library quantification of Illumina next-generation sequences. The kit allows the specific and accurate quantification of libraries bearing P5 and P7 adaptors which can be applied to flow cell amplification. It uses the highly efficient qPCR master mix KOD SYBR® qPCR Mix.
- Code No. NLQ-101 500 reactions