qPCR Probe LyoBeads pre-dispensed (96wells)

Product#: 2201
$158.00

Size:

  • 1 Plate
  • 5 Plates
Availability:
Ships in 1-2 weeks

qPCR Probe LyoBeads pre-dispensed (96wells)

Cat. No. 2201
Size 1 plate / 5 plates
Store 

LyoBeads are shipped and stored simply at room-temperature. This provides a more cost efficient and ecological distribution compared to other master mixes.

Description
qPCR Probe LyoBeads contain all components necessary for rapid, sensitive and reproducible detection and quantification of DNA and cDNA. All necessary components for a reliable PCR are already included in one LyoBead: an engineered DNA polymerase, an optimized reaction buffer and ultrapure dNTPs. A hot-start formulation of the included DNA polymerase prevents false amplification during the reaction set-up.

LyoBeads are ready-to-use, freeze-dried master mixes in shape of a small ball or spheres.

LyoBeads contain: DNA polymerase(s), reaction buffer, dNTPs and primers and probes. They are rehydrated within seconds in any aqueous solutions, which  makes reaction setup very simple. Only a biological sample has to be added.

LyoBeads can be pre-dispensed in PCR-strips, PCR-plates, cartridges etc.

All necessary components for PCR are already included in one LyoBead: an engineered DNA polymerase, an optimized reaction buffer and ultrapure dNTPs. Only primers and probes need to be added. A hot-start formulation of the included DNA polymerase prevents false amplification during the reaction set-up.

Quality Control Assays
qPCR Probe LyoBeads are tested in real-time qPCR. The product demonstrates linearity of amplification over a specified serial dilution of human genomic DNA. DNA polymerase activity: DNA polymerase activity has been monitored and adjusted to a specific DNA polymerase activity using an artificial DNA template and a DNA primer. Enzyme-concentration has been determined by proteinspecific staining. Please inquire more information at info@mypols.de for the lot-specific concentration. No contamination has been detected in standard test reactions.

Safety
This product does not require a Material Safety Data Sheet because it does neither contain more than 1% of a component classified as dangerous or hazardous nor more than 0.1% of a component classified as carcinogenic. However, we generally recommend the use of gloves, lab coats and eye protection when working with these or any other chemical reagents. myPOLS Biotec takes no liability for damage resulting from handling or contact with this product. Further information can be found in the REGULATION (EC) No 1272/2008 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL.

Recommendations for PCR/ Reaction Setup

Component

Volume

LyoBead*

0.75 µl (this is the volume of one LyoBead)

Primers & Probes

x µl

Template / Sample y µl

y µl

Water

fill up to 20 µl total reaction volume

* LyoBeads can be dispensed by using fine metallic tweezers or a small metallic spoon

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