TAMRA-IL-2, Pinpoint-Labeled, Human
Cat. No. RP603
Store at -80°C
Package; 1 µg ( µg/ml solution, 0.1 % (v/w) polyoxyethylene (23) lauryl ether in PBS)
Activity; The ED50 is 0.1-0.2 ng/ml, determined by cell proliferation assay using CTLL-2 cells.
MW 18.6 kDa
Abs/Em* 546/575 nm
*Approximate absorption (Abs) and fluorescence emission (Em) maxima of TAMRA dye
IL-2 (Accession No. P60568), pin-point labeled with TAMRA, was produced in the E. coli cell-free translation system in the presence of TAMRA-C6-paminophenylalanine (TAMRA-aminophenylalanine) amber suppressor tRNA, which is the one of the CloverDirectTM tRNA reagents for site-directed protein labeling1). Using amber suppression2), TAMRA-aminophenylalanine is sitespecifically incorporated at the amber stop codon (UAG) position. TAMRA-IL-2 has the N-terminal ProX tag, which is the sequence tag optimized for high productivity and high introducing efficiency of TAMRAaminophenylalanine, and has the C-terminal hexahistidine tag. The concentration of Pin-point TAMRA-IL-2 was measured by TAMRA fluorescence intensity using with TAMRA dye as a standard. TAMRA-IL-2 was purified by metal chelating-column.
The identical labeled protein, TAMRA-IL-2, is able to be produced by Pin-point Fluorescence Labeling Kit 5433), using with frame shift suppression (CGGG codon) instead of amber suppression.
- Science, 244,182-188 (1989)