INACTIVESignal Booster Solution A&B set (100 mL each)

Product#: xxxBCL-110
$100.00
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Signal Booster Solution A&B set 100mL each
 

Cat. No. BCL-110
Size 1 Set
Storage 4°C 

Description

Much higher signal is desired?
Try the "Signal Booster" and "Signal Booster Neo" !

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"Signal Booster series" are signal enhancement reagent for immunoassays.

The main composition of Signal Booster series are polymer and blocking ingredients.
Polymer alters the surface physico-chemical properties of antigens and antibodies.
This makes antigen and antibody enhance the contact frequency and specific binding.
Combination of polymer and blocking ingredients helps you to get higher signal with lower background.

"Signal Booster" : Standard signal enhancer (two components system) for multiple immunoassays.

*This product has been featured in Nature Market Place.

Feature

  • Ready To Use
    • Simply dilute antibodies by Signal Boster.
    • For Primary antibody, dilute by Solution A.
    • For Secondary antibody, dilute by Solution B.
  • Stronger signal and lower background
    • Can get a few to >10 times stronger signals.
  • Stronger signal as compared to similar products
  • Wide variety of applications
    • Can be used for Western Blot, ELISA, etc.
    • HRP or ALP will not be affected by Signal Booster.
    • Luminecense, Fluorescence detection system is also compatible.
"Signal Booster Neo" : Chemically defined, Protein-free signal enhancer.

Other Feature
  • "Ready To Use" One solution system
    • Simply dilute Primary antibody and Secondary antibody by Signal Booster Neo.
  • Stronger signal and lower background
    • Can get a few to >10 times stronger signals.
  • For phospho protein detection or for any ELISA
    • Protein-free composition contributes to no interference by proteins in the reagent.
  • Wide variety of applications
    • Can be used for Western Blot, ELISA, etc.
    • HRP or ALP will not be affected by Signal Booster.
    • Luminecense, Fluorescence detection system is also compatible
Example Data
(A) Signal Booster is used for detection of Luciferase
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NT : TBS (-), SB : Signal Booster, A : Competitor company product
Sample : Luciferaseexpressing HepG2cell lysate
1st Ab : Anti-Luciferase IgG, goat polyclonal, 1/1000 diluted by Solution A
2nd Ab : Anti-Goat IgG, donkey HRP-labeled, 1/20000 diluted by Solution B


(B) Signal Booster is used for detection of HBsAgS by Sandwich ELISA
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Solid phase Ab:Anti-HBsAg Ab, Rabbit polyclonal
Sample : HBsAg-XT (Cat #:BCL-AG-002 )
1st Ab : Anti-HBsAg Ab, Mouse monoclonal ,diluted by Solution A
or Anti-HBsAg Ab, Mouse monoclonal HRP, diluted by Solution B
2nd Ab : Anti-mouse IgG, Goat polyclonal, HRP, diluted by Solution B


(C) Signal Booster Neo is used for detection of phospho-EGFR
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SB : Signal Booster, SBN : Signal Booster Neo
Sample : A431 Cell Lysate
Blocking : c-Block
1st Ab : Anti-pEGFR, Rabbit
2nd Ab : Anti-Rabbit,Goat polyclonal, HRP


(D) Signal Booster Neo is used for detection of HBs antigen by ELISA
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SB : Signal Booster, SBN : Signal Booster Neo
Blocking : b-block
Capture Ab : Anti-HBsAg mouse
Detection Ab : Anti-Mouse IgG-HRP


Signal Booster
(E) Signal Booster Neo is used for detection of mouse serum amyloid A3 by Western Blot.
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Sample : Mouse Serum Amyloid A3, overexpressed in E.Coli 1st and 2nd Antibodies are diluted in TBST-dried milk or Signal Booster Neo according to manufacturer’s instructions.
There was a dramatic difference with the Signal Booster Neo.
(Data kindly provided by Dr. Robert Anderberg, Providence Medical Research Center, Providence Health Care)
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