Pfu DNA polymerase (+dNTPs), Economy
Pfu DNA polymerase (+dNTPs), Economy, with reaction buffer
Cat. No. 02-021
Store at -20°C
size 200 Units / 5X 200 Units
Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa, same as that of the natural Pfu DNA polymerase.
- Pfu DNA polymerase is thermostabe and has low error rates.
- It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
- Pfu DNA polymerase-generated PCR fragments are blunt-ended.
2.5 units/ul, where one unit is defined as the amount of enzyme that can incorporate 10 nmols of dNTPs into an acid-insoluble material in 30 minutes at 72℃ when activated salmon sperm DNA was used as template/primer.
Greater than 95% of protein determined by SDS-PAGE (CBB staining) (Fig.1) The absence of endonucleases and exonucleases was confirmed.
Good amplification result was obtained in PCR reaction using λDNA as a template (Fig.2).
Reagents Supplied with Enzyme
10 x Reaction Buffer (Pfu): 200mM Tris-HCl (pH 8.8), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1% TritonX-100, 1 mg/ml BSA
2.5mM (each) dNTPs
Fig.1 SDS-PAGE of Pfu DNA polymerase
Fig.2 Amplification ofλDNA
|02-021_02-021-5||5 x 200 units|