Pfu DNA polymerase (+dNTPs), Economy

Product#: 02-021
$100.00

Size:

  • 1X200Units
  • 5X200Units
Availability:
Ships in 1-2 weeks

Pfu DNA polymerase (+dNTPs), Economy, with reaction buffer

Cat. No. 02-021
Store at -20°C
size 200 Units / 5X 200 Units

Description

Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa, same as that of the natural Pfu DNA polymerase. 

Features

  • Pfu DNA polymerase is thermostabe and has low error rates.
  • It is suitable for PCR and primer extension reactions that require high fidelity synthesis. 
  • Pfu DNA polymerase-generated PCR fragments are blunt-ended. 
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Concentration

2.5 units/ul, where one unit is defined as the amount of enzyme that can incorporate 10 nmols of dNTPs into an acid-insoluble material in 30 minutes at 72℃ when activated salmon sperm DNA was used as template/primer.

Quality Assurance

Greater than 95% of protein determined by SDS-PAGE (CBB staining) (Fig.1) The absence of endonucleases and exonucleases was confirmed.

PCR Test

Good amplification result was obtained in PCR reaction using λDNA as a template (Fig.2). 

Reagents Supplied with Enzyme

10 x Reaction Buffer (Pfu): 200mM Tris-HCl (pH 8.8), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1% TritonX-100, 1 mg/ml BSA
 2.5mM (each) dNTPs 

02-031_fig2.jpg

02-031_fig3.jpg

Fig.1 SDS-PAGE of Pfu DNA polymerase 

Fig.2 Amplification ofλDNA




Order Information
 

Cat No. Size
02-021_02-021 200 units
02-021_02-021-5 5 x 200 units








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