Myell

Product#: MYLL
$110.00
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Polymer ultra-thin film for microscope imaging Myell series

For live imaging of floating cells, yeast, bacteria, tissues, etc.


Cat No: See below


Description


The Myell series is an ultra-thin polymer film that can be used as a substitute for cover glass. By lapping the sample with this product, various advantages not available with the existing preparation methods can be obtained.
 
Product features
  • By wrapping with Myell series, observation samples such as floating cells can be retained, image blurring during microscope imaging can be prevented, and clear images can be obtained.
  • By wrapping with Myell D made of CYTOP ® , which has water and oil repellency, shrinkage due to drying of the sample is prevented, and long-time image acquisition such as tiling photography of tissue pieces and 3D imaging You can even take clear images. Myell D also supports observation with an oil immersion lens.
  • By using the porous type Myell P , it is possible to administer a liquid stimulant such as a drug from the outside while keeping floating cells within the field of view of the microscope.
* CYTOP ® is a registered trademark of AGC Chemicals.

Product lineup
 
Product name Myell A 
(Limited to first purchase)
Myell S Myell D Myell P
Type Standard ultra thin film High-performance ultra-thin film Porous ultra thin film
Product code MYLL_A3-One sample only MYLL_S10 MYLL_D10 MYLL_P4
Packaging 3 pieces 10 sheets 10 sheets 4 pieces
Apply Can hold floating cells or tissue         Can hold floating cells or tissue 
            Can prevent desiccation.
       Can hold floating cells or tissue 
           Can add stimulation factor.
Pore None None Yes (pore pitch: 6 μm)
Material Polylactic acid CYTOP ® (water and oil repellency) Polylactic acid
Film thickness 60 nm 120 nm 60 nm
Diameter φ 27 mm φ 27 mm φ 27 mm

* Myell™ series are NOT sterilized
* Note: For Myell™ A, one laboratory can order one time only.



Myell™ A or S: Prevent samples from vibrating
 
When observing cells such as floating cells or yeast, dropping cell suspension on a glass bottom dish is general method.
However, this method has a problem that the picture becomes blurry because the cells vibrate due to their brownian motion.
By wrapping with Myell™ A/S, the samples on a slide grass are prevented from vibrating. This makes us to get clear pictures.
Also, pictures hardly blurry even if some droplets are added on samples wrapped with Myell A/S.

Myell-2.png

 
- Myell Wrapping
 
 


Experimental example

Liposome suspention was wrapped or without wrapped by Myell™ A/S and applied to glass bottom dish.
Some drops were added on the both dishes and compared the pictures.

Without-wrapped
 
Myell-3.png
The liposome was flown out of the field in microscope.


Wrapping by Myell S or A
Myell-4.png
The liposome remained in the field of microscope and did not have much vibrate.



Myell™ D: Prevent samples from desiccation

Myell™ D prevents samples from not only vibrating but also the desiccation and shrink.
These features of Myell™ D is due to the material, CYTOP® which has both water-repellent and oil-repellent.

Then, Myell™ D is useful for long time imaging in a tiling mode and three dimensional mode.
Also, Myell™ D does not influence on image in fluorescence microscope. 
 

Experimental example

The fixed brain section from GFP-expressed mouse was wrapped by Myell™ D or not.
Then after, the imaging was performed by a tiling mode as below.
Myell-5.png

The sample without-wrapped was desiccated and shrinked, consequently, the picture was blurry toward Z axis.
On the other hand, Myell™ D-wrapped sample was prevented from desiccating, which enables to get clear picture.


Myell™ P: Enable to add stimulation factor

Conventionally, it is difficult to observe the effect of stimulation factor during live imaging because the samples are flown out once the stimulation factor is added.
Myell™ P has an advantage that adding stimulation factor on sample is possible while holding sample in microscopic field.
This is because Myell™ P has many small porous to penetrate.
 
Myell™ P has many small porous

The cells cannot penetrate this porous, however, the stimulation factor can penetrate.
Therefore, the cells remain inside and the effect of stimulation factor can be observed.
Myell-6.png

 


How to use
 
1. Place coverslip(diameter: =< 25 mm) horizontally on a stable stage
2. Place specimen on the coverslip
3. Pass the specimen though Myell (press Myell-film on the specimen and break through the film), then the specimen is wrapped. 

Myell-7.png



*In case of using upright microscope
Please put sample which wrapped with Myell™ on slide grass, petri dish or directly on stage of microscope.
Upright microscope is suitable for Myell™ A/S and Myell™ D.
 
*In case of using inverted microscope
Please put sample which wrapped with Myell™ on petri dish.
This method is possible to soak water or embedded in agarose gel to prevent desiccation.
Inverted microscope is suitable for all Myell™ series.
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