Lithium Assay kit LS-Polyfluoroporphyrin method

Product#: LI01ME
$680.00
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Lithium Assay kit LS-Polyfluoroporphyrin method

Cat. No. LI01ME
Size 100 tests / 1 kit
Store oC
Measuring range  0.03 - 30 mM
Wavelength (maximume wavelength) 540 - 560 nm (550 nm)
Chromogen Polyfluoro-porphyrin
Target Copper Lithium (Li , Li2 CO3)

Description

Lithium carbonate has been used for treatment of bipolar disorder. However, Lithium carbonate is absorbed by a gastrointestinal and overdose of a lithium may occur nephropathy,serum lithium levels higher than 1.5 mM indicate a significant risk of intoxication.

Therefore, supervised administration of lithium carbonate is required and important.

Lithium carbonate used in the treatment of bipolar disorder. Lithium carbonate is absorbed by a gastrointestinal. The overdose of a lithium is occur nephropathy. Therefore and this medication manage are required. Serum lithium levels higher than 1.5 mM indicate a significant risk of intoxication. This Lithium reagent kit is a chromogenic method. Lithium present in the serum sample give a magenta colored complex with polyfluoroporphyrin (as chromogen).

The color intensity is proportional to the amount of lithium present in the sample. This product can perform stable assay under ordinary temperature and atmospheric pressure. No need gas barrier function enable us to use all type of measuring equipment including existing autoanalyzer. Excellent storage stability (even after opening sealed package) gives extended validated date.

 *Patent number. JP 5100903, JP 5222432

Features

  • Using conventional microplate reader for the assay. No gas barrier equipment is required.
  • Good correlation with the result of Atomic Absorption Spectrometry
  • Sample pre-treatment is not required.
  • Kit does not contain any hazardous components.
  • 1-point calibration
  • Li and Li2CO3 can be measured.
  • Applicable sample Type : Serum and Plasma (Heparin Lithium sample cannot be used.)
  • Species : All species
  • Assay range : 0.03 ~ 3.0 mM
  • Assay time : 10 minutes
Assay Principal

Lithium present in the serum sample gives a magenta colored complex with polyfluoroporphyrin (as chromogen). The color intensity is proportional to the amount of lithium present in thesample. 

Notes

  • Unstableness of incubation temperature may result in unstable results.
  • Use disposable test tube and glassware washed with 1M HNO3 or 1M HCl solution and distilled water.
  • Accuracy in pipetting volume for samples and reagents may affect the quality of assay. Please note that samples, standards and reagents must be poured accurately µL level.
  • Temperature for chromogen reaction may affect optical density. Please try to extend or shorten chromogen reaction time depending on room temperature.
  • Heparin lithium anticoagulant affects to chromogenic system. The best samples are EDTA plasma or citric acid plasma, and non-hemolyzed serum

Operation

1. Sample preparation

Serum or Plasma
Insoluble substances in serum and plasma samples should be
removed by filtration or centrifugation.

2. Assay preparation

Bring all reagents to room temperature before use

3. Assay procedure.

Procedure using microplate reader.
(1 assay sample 244µL)
?Assay
(1) Add 4 µL of Distilled water (Blank) / STD (Standard)/ sample into each well.
(2) Add 240 µL of R-R to each well and incubate at room temperature for 10 min.
(3) Read the absorbance at 550 nm (main) and 600 nm (sub). --> OD

*Select the filter: 540-560 nm at 550nm, 600-610 nm at 600 nm.

Expiration date and preservation conditions

Storage conditions: Store at 2-8°C. Don’t freeze.
Expiration: 1 year from the date of manufacture. After the bottles are opened, the kit should be used in 1 month.

Correlation data between this kit and Atomic Absorption Spectrometry method

LI01ME_fig2.jpg 

Performance

Measuring range 0.03 – 3.0 mM
Imprecision Imprecision was evaluated using commercially available quality control serum.

Within run

 

Mean mM

S.D

C.V

Level 1

0.72

0.03

3.26

Level 2

1.47

0.04

2.68


Interferences
No interference by the note of substances were observed.
[Observed 500nm only]
Conjugated bilirubin and unconjugated bilirubin 40 mg/dL
Hemoglobin 300mg/dL Chyle 300FTU
[Observed 500nm with 600nm]
Conjugated bilirubin and unconjugated bilirubin 40 mg/dL
Hemoglobin 1 g/dL Chyle 3,000 FTU

Expiration date and preservation conditions
Storage conditions: Store at 2-8°C. Don’t freeze.
Expiration: 1 year from the date of manufacture. After the bottles are opened, the kit should be used in 1 month.

Reference
  1. Synthesis of F28 tetraphenylporphyrin and its application to the separation and detection of lithium(Ⅰ). Kenji KOYANAGI, Masaaki TABATA, BUNSEKI KAGAKU; ISSN:0525-1931;vol.51, No.9, pp803-807(2002)
  2. Patent number. JP 5100903, JP 5222432
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