GreenDye 20x

Product#: 2000
$80.00

Size:

  • 250 ul
  • 1000 ul
Availability:
Ships in 1-2 weeks

GreenDye 20x

Cat. No. 2000
Store 

This product is shipped in cool packs. Please store the product upon arrival at - 20 °C. Minimize the number of freeze-thaw cycles by storing in aliquots. For a day-to-day use, we recommend keeping an aliquot at 4 °C.
 

Product Name

Cat. No.

Size

Reaction

Storage

Price USD

GreenDye 20×

2000

250 µl

200 reactions

-20 oC

80.00

GreenDye 20×

2000

1000 µl

800 reactions

240.00


Description
Boost your qPCR results without inhibition with GreenDye 
GreenDye is a highly sensitive DNA binding dye suitable for qPCR applications and melting curve analysis.
GreenDye shows very low DNA polymerase inhibition when compared with other dyes in PCR. It features a very high fluorescence when bound to double-stranded DNA and exhibits no degradation during PCR cycling. It is a perfect dye for qPCR and high resolution melting applications.

Features
  • Thermally stable for 50 cycles and more
  • Suited for qPCR, melt curve analysis and High Resolution Melting (HRM)
  • No decrease in DNA polymerase performance at 1x concentration
  • Optimized for PCR experiments with HiDi DNA polymerase and HiDi Taq DNA polymerase
Applications
  • real-time PCR (FAM channel)
  • melt curve analysis
  • High Resolution Melting (HRM)
  • qPCR
  • real-time primer extension
  • screening
Reaction Setup
It is recommended to use GreenDye at 1x concentration.
For example, in a 25 µl PCR reaction 1.25 μl GreenDye is used for each reaction. The fluorescence is measured at the usual SYBR/FAM channels.

Example Results
Benchmarking GreenDye against competitors
The fluorescence of GreenDye is benchmarked using a qPCR assay for the detection of a sequence in genomic HeLa DNA. PCRs were performed with 1 ng/µl of HeLa gDNA in the presence of either GreenDye 1x, EvaGreen® 1x or SYBR® Green I 1x in combination with HiDi DNA polymerase.

The real-time PCR curve with 1x GreenDye (green) in comparison with 1x EvaGreen (grey) and 1x SYBR® Green I (blue) shows the lowest ct-value.
2000_Example1.png 
GreenDye Melt Curve Analysis
The melting curves are acquired from the  PCR experiment described above. All three dyes show a single peak indicating a single amplification product. GreenDye however, shows the sharpest melting peak.
2000_Example2.png 
Recommendations
It is recommended to use GreenDye at 1x concentration. 
For example, add 1.25 μl GreenDye to a 25 µl PCR reaction. The fluorescence is measured at the usual SYBR/FAM channels.

Content of the Product
20x GreenDye in a aqueous solution

Quality Control
GreenDye 20x is tested together with HiDi DNA polymerase (#9001) in a real-time PCR. A sequence in genomic HeLa DNA is used as a target to validate ct- and fluorescence values of GreenDye at 1x concentration.
No contamination has been detected in standard test reactions.
Logo_mypols.gif

Satisfaction
Quality Rating
Value Rating
Style Rating
X